Protein quantification process using Opentrons Flex magnetic bead protein purification workstation

In modern biological laboratories, Opentrons Flex magnetic bead protein purification workstations are popular for their efficiency and automation. One of the core functions of this workstation is protein quantification, a process critical to ensuring the accuracy of experimental results.

Opentrons Flex Magnetic Bead Protein Purification Workstation

1. Experimental preparation 1. Equipment and material preparation: Opentrons Flex magnetic bead protein purification workstation 96-well transparent flat bottom plate pipette, pipette and other working reagents and test samples BioTek Synergy H1 plate reader (or other compatible plate reader magnetic bead purification module (if magnetic bead purification is required) 2. Reagents and sample preparation: According to experimental needs, prepare appropriate concentrations of protein standards and samples to be tested. Prepare required Working reagents, such as BCA reagent (A solution and B solution), Bradford reagent, etc.

2. Experimental settings 1. Programming settings: On the Opentrons Flex workstation, perform programming settings according to experimental requirements. This includes setting parameters such as the pipette's movement path, pipetting volume, and incubation time. 2. Reagent and sample distribution: Use the pipetting function of the Opentrons Flex workstation to distribute working reagents and test samples into each well of the 96-well plate. Ensure accurate and consistent amounts of reagents and sample in each well.

3. Experiment execution 1. Mixing and incubation: Mix the distributed reagent and sample mixture on the thermal shaking module of Opentrons. Usually the mixing time is about 30 seconds. The mixture is incubated at an appropriate temperature for a period of time based on experimental needs. For example, the BCA assay requires incubation at 37°C to ensure signal strength for detection. 2. Magnetic bead purification (if necessary): If the experiment requires magnetic bead purification, you can use the magnetic bead purification module of the Opentrons Flex workstation. The mixture containing the magnetic beads is added to an appropriate container, and a magnetic field is used to separate the beads and protein complexes.

4. Result detection and analysis 1. Absorbance measurement: Use BioTek Synergy H1 plate reader (or other compatible plate readers) to analyze the result plate. Measure the absorbance value of each well, usually selecting a specific wavelength for measurement (such as 562nm for the BCA method). 2. Data processing and analysis: Calculate the protein concentration using appropriate formulas or methods based on the absorbance value. If a standard is used, a standard curve can be drawn and the protein concentration of the sample to be tested can be calculated based on the standard curve. Perform statistical analysis on the data to evaluate the accuracy and reliability of the experiment.

5. End of experiment and cleanup 1. Clean the workstation: After the experiment, clean the Opentrons Flex workstation and related experimental equipment in a timely manner. Properly dispose of used reagents and samples to avoid harm to the environment and personnel. 2. Record and save data: Record the experimental results and data, and save and back them up appropriately. This facilitates subsequent data analysis and experimental validation.

Please note that specific experimental steps and parameters may vary depending on experimental needs, reagent types, and instrument models. Therefore, before conducting experiments, it is recommended to read the instruction manual and related experimental guides of the Opentrons Flex Magnetic Bead Protein Purification Workstation in detail to ensure the accuracy and reliability of the experiment.

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