A 12-channel pipette can be added to a 384-well plate. The combination of 12-channel pipettes and 384-well plates plays a vital role in high-throughput experiments. They not only improve experimental efficiency, but also ensure the accuracy of experimental results. The advantage of this combination is not only reflected in the saving of time and labor, but also its ability to process a large number of samples, which is undoubtedly a benefit for researchers who need to perform high-throughput screening, PCR, ELISA, cell culture and other experiments. A huge boon.
1. Introduction to 12-channel pipette and 384-well plate 1. The design of the 12-channel pipette enables it to process 12 samples at the same time. This feature is particularly important when facing a large number of samples. By adjusting the channel spacing of the pipette, it can be easily adapted to different plate sizes, including 384-well plates. This flexibility allows researchers to quickly and accurately complete sample addition, mixing, transfer and other operations according to different experimental needs, thus greatly improving experimental efficiency. 2. As a high-throughput laboratory product, the number and arrangement of the microwells in the 384-well plate are carefully designed to adapt to the operation of high-throughput instruments. Each microwell has a specific diameter, depth and spacing to ensure stability and uniformity of the sample within it. This design not only improves the accuracy of experiments, but also makes it easier for researchers to manage and analyze experimental results.
2. When using 12-channel pipettes and 384-well plates, you need to pay attention to the following points: 1. Calibration and maintenance of pipettes are crucial. Regular calibration ensures your pipette's accuracy, while routine maintenance and care can extend its life. Before use, check whether all parts of the pipette are intact and whether the tip is tightly installed to avoid problems during the experiment. 2. Select the appropriate pipette model and configuration according to experimental needs. Different experiments may require pipettes with different ranges and precisions. Therefore, when selecting a pipette, the specific requirements of the experiment should be fully considered. 3. When using 384-well plates, care should be taken to avoid contamination and cross-contamination. Before each use, make sure the plate is clean, dust-free, and free of residue. During the sample addition process, a dedicated pipette or sampler should be used, and strict sample addition sequence and operating procedures should be followed. 4. After the experiment, the pipette and 384-well plate should be cleaned and disinfected in time to avoid the growth of bacteria and other contaminants. At the same time, they should be stored in a dry, cool, and ventilated place to maintain their good performance and service life.
3. How to use the 384-well plate 1. Add sample: Use a pipette or automatic sampler to add experimental reagents to the wells of the 384-well plate in sequence. Pay attention to avoid cross-contamination when adding samples, and ensure that the amount of sample added to each well is consistent. 2. Sealing film: Use special optical film or heat sealing film to seal the 384-well plate. Make sure the membrane fits tightly to the 384-well plate and has no bubbles or wrinkles. 3. Place the experimental instrument: Place the sealed 384-well plate into the corresponding experimental instrument, such as a PCR machine, microplate reader, etc. Carry out the experiment according to the preset procedure. 4. Result analysis: After the experiment is completed, take out the 384-well plate and use the corresponding instrument to analyze the experimental results. 5. Note: The 384-well plate is a disposable consumable and is not recommended for reuse. During use, care should be taken to avoid contamination of the 384-well plate. 384-well plates should be stored in a dry, dust-free environment to avoid moisture and contamination.
4. Matching of pipette and well plate 1. Single-channel pipette: Since only one sample can be processed at a time, single-channel pipette is suitable for processing small-scale experiments, such as adding and mixing a single sample. Wait for operations. For well plates, single-channel pipettes can be applied to well plates of any size, but are less efficient. 2. Multi-channel pipette: Multi-channel pipette can process multiple samples at the same time, thus greatly improving experimental efficiency. For well plates, the number of channels in the multichannel pipette should match the number of wells in the well plate. For example, an 8-channel pipette is suitable for a 96-well plate (8 wells per row or column), while a 16- or 24-channel pipette is better suited for a 384-well plate (more wells per row or column). hole).
The combination of 12-channel pipettes and 384-well plates plays an indispensable role in high-throughput experiments. They not only improve experimental efficiency and accuracy, but also provide researchers with more convenient and efficient experimental methods. During use, attention needs to be paid to the calibration and maintenance of the pipette, as well as the standardization and safety of experimental operations to ensure the accuracy and reliability of experimental results.
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