{"id":8737,"date":"2025-03-10T17:14:59","date_gmt":"2025-03-10T09:14:59","guid":{"rendered":"https:\/\/opentrons.com.cn\/news\/\/"},"modified":"2025-03-10T17:15:25","modified_gmt":"2025-03-10T09:15:25","slug":"elisa-ceshiliucheng","status":"publish","type":"post","link":"https:\/\/opentrons.com.cn\/ko\/news\/elisa-ceshiliucheng\/","title":{"rendered":"ELISA \ud14c\uc2a4\ud2b8 \ud504\ub85c\uc138\uc2a4\uc5d0 \ub300\ud55c \uc790\uc138\ud55c \uc124\uba85"},"content":{"rendered":"\n
ELISA (\ud6a8\uc18c-\uc5f0\uacb0 \uba74\uc5ed \ud761\ucc29\uc81c \ubd84\uc11d)\ub294 \uc0dd\ubb3c \uc758\ud559 \uc5f0\uad6c\uc5d0\uc11c \uc77c\ubc18\uc801\uc73c\ub85c \uc0ac\uc6a9\ub418\ub294 \uc2e4\ud5d8 \ubc29\ubc95\uc774\uba70 \ud56d\uccb4, \ud56d\uc6d0, \ud638\ub974\ubaac, \ubc14\uc774\ub7ec\uc2a4 \ub4f1\uc758 \uac80\ucd9c\uc5d0 \ub110\ub9ac \uc0ac\uc6a9\ub41c\ub2e4. ELISA \ud14c\uc2a4\ud2b8\ub97c \ud1b5\ud574 \uc5f0\uad6c\uc6d0\ub4e4\uc740 \uc815\ub7c9\uc801 \ub610\ub294 \uc9c8\uc801 \ubd84\uc11d \uacb0\uacfc\ub97c \uc5bb\uc744 \uc218\uc788\uc5b4 \uc784\uc0c1 \uc9c4\ub2e8, \uc9c8\ubcd1 \uac10\uc2dc \ubc0f \uc57d\ubb3c \uac1c\ubc1c\uc5d0\uc11c \ub300\uccb4 \ud560 \uc218 \uc5c6\uc2b5\ub2c8\ub2e4.<\/p>\n\n\n\n
<\/p>\n\n\n\n Elisa Detection <\/a><\/p>\n\n\n\n 1. \ucf54\ud305 \ub2e8\uacc4 <\/strong> ELISA \uac10\uc9c0\uc758 \uccab \ubc88\uc9f8 \ub2e8\uacc4\ub294 \ucf54\ud305 \ub41c \ud50c\ub808\uc774\ud2b8\uc5d0\uc11c \ud14c\uc2a4\ud2b8 \ud560 \uae30\ubcf8 \uad6c\uc131 \uc694\uc18c\ub97c \uace0\uc815\ud558\ub294 \uac83\uc785\ub2c8\ub2e4. \uc774 \uacfc\uc815\uc740 \uc8fc\ub85c \uc218\ub3d9 \ud761\ucc29\uc744 \ud1b5\ud574 \ub2ec\uc131\ub418\uba70, \uadf8 \ud6a8\uacfc\ub294 \ucf54\ud305 \ubb3c\uc9c8\uc758 \ud2b9\uc131\uc5d0 \ub2ec\ub824 \uc788\uc2b5\ub2c8\ub2e4. \uc0d8\ud50c\uc774 \ub2e4\uc218\uc758 \ud56d\uc6d0\uc744 \ud568\uc720\ud558\ub294 \uacbd\uc6b0, \ube44\ud2b9\uc774\uc801 \uacb0\ud569\uc744 \ud53c\ud558\uae30 \uc704\ud574 \uc120\ud0dd\uc801 \ucf54\ud305 \ub41c \ud50c\ub808\uc774\ud2b8\ub97c \uc120\ud0dd\ud574\uc57c\ud55c\ub2e4. \uadf8\ub7ec\ub098, \uac04\uc811 ELISA \ubc29\ubc95\uc5d0\uc11c \ud56d\uccb4\ub97c \uac80\ucd9c \ud560 \ub54c, \uc815\uc81c \ub41c \ud56d\uc6d0\uc774 \uc0ac\uc6a9\ub418\uac70\ub098 \uc0cc\ub4dc\uc704\uce58 ELISA \ubc29\ubc95\uc5d0\uc11c \ucea1\ucc98 \ud56d\uccb4\ub97c \uc0ac\uc6a9 \ud560 \ub54c, \ucf54\ud305 \ub41c \ud50c\ub808\uc774\ud2b8\ub294 \uc774\ubbf8 \uc120\ud0dd\uc801\uc774\ub2e4. \ucf54\ud305 \ub41c \ubcf4\ub4dc\uc758 \uc7ac\ub8cc\ub294 \ub300\ubd80\ubd84 \ud3f4\ub9ac\uc2a4\ud2f0\ub80c \ub610\ub294 \uadf8 \uc720\ub3c4\uccb4\uc774\uba70, \uacb0\ud569 \ud2b9\uc131\uc740 \ud45c\uc801 \ubb3c\uc9c8 \ubc0f \uac80\ucd9c \uc694\uad6c \uc0ac\ud56d\uc5d0 \ub530\ub77c \ub2e4\ub985\ub2c8\ub2e4. \uace0\ub3c4\ub85c \uae00\ub9ac\ucf54 \uc2e4\ud654 \ub2e8\ubc31\uc9c8, \ud0c4\uc218\ud654\ubb3c \ub4f1\uacfc \uac19\uc774 \ud761\ucc29\ud558\uae30 \uc5b4\ub824\uc6b4 \ud2b9\uc815 \ubb3c\uc9c8\uc758 \uacbd\uc6b0 \uacf5\uc720 \uacb0\ucc30\uc744 \ud5c8\uc6a9\ud558\ub294 \ud2b9\uc218 \ucc98\ub9ac \ucf54\ud305 \ud50c\ub808\uc774\ud2b8\ub97c \uc0ac\uc6a9\ud558\ub294 \uac83\uc774 \uc88b\uc2b5\ub2c8\ub2e4.<\/p>\n\n\n\n 2. \uc778\ud050\ubca0\uc774\uc158 \ub2e8\uacc4 <\/strong> \uc2dc\uc57d\uc744 \ucf54\ud305 \ud50c\ub808\uc774\ud2b8\uc5d0 \ucca8\uac00 \ud55c \ud6c4, \uc778\ud050\ubca0\uc774\uc158\uc740 \uacb0\ud569 \ub610\ub294 \ubc18\uc751\uc744 \ucd09\uc9c4\ud574\uc57c\ud569\ub2c8\ub2e4. \uc778\ud050\ubca0\uc774\uc158 \uc628\ub3c4\uc640 \uc2dc\uac04\uc740 \uac80\ucd9c \ub2e8\uacc4 \ubc0f \uc791\uc5c5\uc5d0 \ub530\ub77c \ub2e4\ub985\ub2c8\ub2e4. \uc804\ud615\uc801\uc73c\ub85c, \uc801\uc6a9 \ud6c4 \uc0d8\ud50c\uc744 37 \u2103\uc5d0\uc11c 1 \uc2dc\uac04 \ub3d9\uc548 \uc778\ud050\ubca0\uc774\uc158\ud55c\ub2e4. \uadf8\ub7ec\ub098, \ucc28\ub2e8\uacfc \uac19\uc740 \ud2b9\uc815 \ub2e8\uacc4\ub294 \ub0c9\uc7a5\uace0\uc5d0\uc11c \ubc24\uc0c8 \uc218\ud589 \ub420 \uc218\uc788\ub2e4.<\/p>\n\n\n\n 3. \uc138\ucc99 \ub2e8\uacc4 <\/strong> \uc138\ucc99\uc740 ELISA \uac10\uc9c0\uc5d0\uc11c \uc911\uc694\ud55c \ub2e8\uacc4\uc785\ub2c8\ub2e4. \uc138\ucc99\ud558\ub294 \ub3d9\uc548, \ud3ec\uc2a4\ud398\uc774\ud2b8 \uc644\ucda9 \uc2dd\uc5fc\uc218 -20 (PBST)\uc740 \uc77c\ubc18\uc801\uc73c\ub85c \uc644\ucda9\uc81c\ub85c \uc0ac\uc6a9\ud558\uc5ec \uacb0\ud569\ub418\uc9c0 \uc54a\uc740 \ud56d\uc6d0, \ud56d\uccb4 \ub610\ub294 \uc2dc\uc57d\uc744 \uc81c\uac70\ud569\ub2c8\ub2e4. \ub2e4 \ucc44\ub110 \ud53c\ud3ab \ub610\ub294 \uc790\ub3d9 \ud50c\ub808\uc774\ud2b8 \uc138\ud0c1\uae30\ub97c \uc0ac\uc6a9\ud558\uc5ec \uc138\ucc99\uc744 \uc218\ub3d9\uc73c\ub85c \uc218\ud589 \ud560 \uc218 \uc788\uc2b5\ub2c8\ub2e4. \ubd80\uc801\uc808\ud55c \uc138\ucc99\uc740 \ubc30\uacbd \uc2e0\ud638\uac00 \ub192\uc744 \uc218 \uc788\uc9c0\ub9cc \uacfc\ub3c4\ud55c \uc138\ucc99\uc740 \uc0d8\ud50c \uc2e0\ud638\ub97c \ub0ae\ucd9c \uc218 \uc788\uc2b5\ub2c8\ub2e4. \uc138\ucc99\uc758 \uc77c\uad00\uc131\uc740 \ud50c\ub808\uc774\ud2b8 \uacb0\uacfc\uc758 \uc2e0\ub8b0\uc131\uc744 \ubcf4\uc7a5\ud558\uae30 \uc704\ud574 \uc911\uc694\ud569\ub2c8\ub2e4.<\/p>\n\n\n\n iv. \ucc28\ub2e8 \ub2e8\uacc4 <\/strong> \ub2e8\ubc31\uc9c8\uc774 ELISA \ud50c\ub808\uc774\ud2b8\ub85c \ucf54\ud305 \ud55c \ud6c4, \ucc28\ub2e8\uc740 \uc77c\ubc18\uc801\uc73c\ub85c \ud6c4\uc18d \ub2e8\uacc4\uc5d0\uc11c \ud56d\uccb4\uc758 \ube44\ud2b9\uc774\uc801 \uacb0\ud569\uc744 \ubc29\uc9c0\ud558\uae30 \uc704\ud574 \ud544\uc694\ud569\ub2c8\ub2e4. \ucc28\ub2e8 \ub420 \ub54c, \uac80\ucd9c\uacfc \uad00\ub828\uc774\uc5c6\ub294 \ud63c\ud569 \ub2e8\ubc31\uc9c8\uc740 \ud50c\ub808\uc774\ud2b8\uc5d0 \ucca8\uac00\ub418\uace0 \ube44\ud2b9\uc774\uc801 \uacb0\ud569 \ubd80\uc704\ub97c \uc810\uc720\ud558\ub3c4\ub85d \uc778\ud050\ubca0\uc774\uc158\ub41c\ub2e4. \uc77c\ubc18\uc801\uc73c\ub85c \uc0ac\uc6a9\ub418\ub294 \ucc28\ub2e8 \uc644\ucda9\uc81c\uc5d0\ub294 \ud0c8\uc9c0 \uac74\uc870 \uc6b0\uc720, \uc18c \ud608\uccad \uc54c\ubd80\ubbfc (BSA) \ubc0f \uce74\uc81c\uc778\uc774 \ud3ec\ud568\ub429\ub2c8\ub2e4. \ub610\ud55c, Tween 20 \ub610\ub294 Triton X-100\uacfc \uac19\uc740 \ube44 \uc774\uc628 \uc138\uc81c\ub294 \ub610\ud55c \ucc28\ub2e8\uc81c\ub85c \uc0ac\uc6a9\ub420 \uc218 \uc788\uc9c0\ub9cc, \ucc28\ub2e8 \ud6a8\uacfc\ub294 \ub2e8\ubc31\uc9c8\ub9cc\ud07c \uc624\ub798 \uc9c0\uc18d\ub418\uc9c0 \uc54a\uc2b5\ub2c8\ub2e4. \ube44\ud6a8\uc728\uc801 \uc778 \ucc28\ub2e8\uc740 \ubc30\uacbd \ub178\uc774\uc988\ub97c \uc99d\uac00\uc2dc\ucf1c \uac10\uc9c0 \uac10\ub3c4\uc640 \ud2b9\uc774\uc131\uc744 \uc904\uc77c \uc218 \uc788\uc2b5\ub2c8\ub2e4.<\/p>\n\n\n\n 5. \ud56d\uccb4 \ub2e8\uacc4 <\/strong> \ud56d\uccb4\ub294 ELISA \uac80\ucd9c\uc758 \uc8fc\uc694 \uad6c\uc131 \uc694\uc18c\uc774\uba70, \uc62c\ubc14\ub978 \ud56d\uccb4\ub97c \uc120\ud0dd\ud558\ub294 \uac83\uc774 \uc911\uc694\ud569\ub2c8\ub2e4. \ub2e8\uc77c \ud074\ub860 \ud56d\uccb4 \ubc0f \ud3f4\ub9ac \ud074\ub85c \ub0a0 \ud56d\uccb4\ub294 \uac01\uac01 \uc7a5\uc810\uacfc \ub2e8\uc810\uc744 \uc0ac\uc6a9\ud558\uc5ec \uc0ac\uc6a9\ub420 \uc218\uc788\ub2e4. \ubaa8\ub178\ud074\ub85c \ub0a0 \ud56d\uccb4\ub294 \ub9e4\uc6b0 \ud2b9\uc774 \uc801\uc774\uc9c0\ub9cc \ud3f4\ub9ac \ud074\ub85c \ub0a0 \ud56d\uccb4\ub294 \ub2e4\uc218\uc758 \uacb0\ud569 \uc9c0\uc810\uc5d0\uc11c \ud45c\uc801 \ubb3c\uc9c8\uc5d0 \uacb0\ud569\ud558\uc5ec \uc2e0\ud638\ub97c \uc99d\ud3ed\uc2dc\ud0a4\uace0 \ubbfc\uac10\uc131\uc744 \ud5a5\uc0c1\uc2dc\ud0ac \uc218\uc788\ub2e4. 2 \ucc28 \ud56d\uccb4\uc758 \uc0ac\uc6a9\uc740 \ub2e8\uacc4\uc640 \uac80\ucd9c \uc2dc\uac04\uc744 \uc99d\uac00 \uc2dc\ud0a4\uc9c0\ub9cc \ubbfc\uac10\ub3c4\uac00 \uc99d\uac00 \ud560 \uc218 \uc788\uc2b5\ub2c8\ub2e4. \ub530\ub77c\uc11c \uac80\ucd9c\uc744 \ucd5c\uc801\ud654 \ud560 \ub54c\ub294 \uc801\uc808\ud55c \ud56d\uccb4 \uc30d\uc744 \ucc3e\uc544\uc57c\ud569\ub2c8\ub2e4.<\/p>\n\n\n\n vi. \uacb0\uc815 \ub2e8\uacc4 <\/strong> \uc5b4\ub5a4 \uc720\ud615\uc758 ELISA \ud14c\uc2a4\ud2b8\uac00 \uc0ac\uc6a9\ud558\ub4e0 \ub9c8\uc9c0\ub9c9 \ub2e8\uacc4\ub294 \uce21\uc815\uc785\ub2c8\ub2e4. \uac00\uc7a5 \uc77c\ubc18\uc801\uc73c\ub85c \uc0ac\uc6a9\ub418\ub294 \ubd84\uc11d\ubc95\uc740 \uac00\uc2dc \uc0c9\uc0c1 \ubcc0\ud654\uc758 \ud6a8\uc18c-\ub9e4\uac1c \ud654\ud559 \ubc18\uc751\uc744 \uc0ac\uc6a9\ud55c \ub2e4\uc74c UV-Vis \ubd84\uad11 \uad11\ub3c4\uacc4\uc5d0 \uc758\ud55c \uce21\uc815\uc744 \uc0ac\uc6a9\ud558\ub294 \uac83\uc785\ub2c8\ub2e4. \ud6a8\uc18c \uacb0\ud569 \ud56d\uc6d0 \ub610\ub294 \ud56d\uccb4\ub97c \uc2dc\ud5d8\uc5d0 \uc798 \ucca8\uac00\ud558\uace0 \uadf8\uac83\uc774 \uc874\uc7ac\ud558\ub294 \uacbd\uc6b0 \ud45c\uc801 \ubb3c\uc9c8\uc5d0 \uacb0\ud569\ub41c\ub2e4. \uc801\uc808\ud55c \uae30\uc9c8\uc774 \ucca8\uac00 \ub420 \ub54c, \ud6a8\uc18c\ub294 \ud45c\uc801 \ubb3c\uc9c8\uc758 \uc591\uc5d0 \ube44\ub840\ud558\ub294 \uc0c9 \ubcc0\ud654\ub97c \uc720\ubc1c\ud55c\ub2e4. \uc591 \uace0\ucd94 \ub0c9\uc774 \ud37c \uc625\uc2dc \ub2e4\uc81c (HRP)\ub294 \uc77c\ubc18\uc801\uc73c\ub85c \uc0ac\uc6a9\ub418\ub294 \ucee8\uc96c \uac8c\uc774\ud2b8 \uc911 \ud558\ub098\uc774\uba70 \uc77c\ubc18\uc801\uc73c\ub85c \uae30\uc9c8 3,3 ', 5,5'- \ud14c\ud2b8\ub77c \uba54\ud2f8 \ubca4\uc9c0\ub518 (TMB)\uacfc \ud568\uaed8 \uc0ac\uc6a9\ub429\ub2c8\ub2e4. HRP\uc758 \uc791\uc6a9\ud558\uc5d0, TMB\ub294 \ud30c\ub780\uc0c9\uc5d0\uc11c \ub178\ub780\uc0c9\uc73c\ub85c \ubc14\ub00c\uc5c8\uace0, \ud669\uc0b0 \uc6a9\uc561\uc744 \ucca8\uac00\ud558\uc5ec \ubc18\uc751\uc744 \uc885\uacb0\uc2dc\ucf30\ub2e4. \ub9c8\uc9c0\ub9c9\uc73c\ub85c, \uac01 \uc6f0\uc758 \ud761\uad11\ub3c4 \uac12\uc740 \ub9c8\uc774\ud06c\ub85c \ud50c\ub808\uc774\ud2b8 \ub9ac\ub354\ub97c \uc0ac\uc6a9\ud558\uc5ec 450nm \ub300\uc5ed\uc5d0\uc11c \uce21\uc815\ub418\uc5c8\uace0, \uc608\ub97c \ub4e4\uc5b4 \ube14\ub7ad\ud06c \uae30\uacf5\uc758 \ud3c9\uade0\uc744 \ube7c\uae30, \uae30\uc220\uc801 \ubc18\ubcf5\uc758 \ud3c9\uade0 \ub610\ub294 \ud45c\uc900 \uacc4\uc0b0\uc758 \ube44\uc728\uacfc \uac19\uc740 \uac80\ucd9c \uc124\uacc4\uc5d0 \ub530\ub77c \ubcf4\uc815 \ubc0f \uacc4\uc0b0\ub418\uc5c8\uc2b5\ub2c8\ub2e4.<\/p>\n\n\n\n ELISA \ud0d0\uc9c0\ub294 \ub2e4\uc591\ud55c \uc758\ub8cc \uc5f0\uad6c \ubc0f \uc9c4\ub2e8\uc5d0 \ub110\ub9ac \uc0ac\uc6a9\ub418\ub294 \ubcf5\uc7a1\ud558\uc9c0\ub9cc \ub9e4\uc6b0 \uc911\uc694\ud55c \uc2e4\ud5d8 \ubc29\ubc95\uc785\ub2c8\ub2e4. ELISA \ud14c\uc2a4\ud2b8 \uacfc\uc815\uc744 \uc774\ud574\ud558\uace0 \uc2b5\ub4dd\ud568\uc73c\ub85c\uc368 \uacfc\ud559 \uc5f0\uad6c\uc790\ub4e4\uc740 \uad00\ub828 \ub370\uc774\ud130\ub97c \uc815\ud655\ud558\uace0 \uc2e0\uc18d\ud558\uac8c \uc5bb\uc744 \uc218\uc788\uc5b4 \uc9c8\ubcd1 \ubc0f \uc57d\ubb3c \ubc1c\ub2ec\uc758 \uc870\uae30 \uc9c4\ub2e8\uc744\uc704\ud55c \uacfc\ud559\uc801 \uae30\ucd08\ub97c \uc81c\uacf5 \ud560 \uc218 \uc788\uc2b5\ub2c8\ub2e4.<\/p>\n\n\n\n \uad00\ub828 \uc77d\uae30 \uad8c\uc7a5 \uc0ac\ud56d<\/p>\n\n\n\n opentrons flex \uc804\uc1a1 \ud578\ub4e4 <\/a><\/p>\n\n\n\n \ub354 \ub098\uc740 \uc0dd\ubb3c\ud559\uc801 \uc2e4\ud5d8\uc2e4 \uacb0\uacfc\ub97c \uac00\uc838 \uc624\uae30 \uc704\ud574 \uc790\ub3d9\ud654\ud558\ub294 3 \uac00\uc9c0 \ubc29\ubc95 <\/a><\/p>\n\n\n\n NGS \uc6cc\ud06c \ud50c\ub85c\uc6b0\ub97c \uc790\ub3d9\ud654\ud558\ub294 5 \uac00\uc9c0 \uc774\uc720 <\/a><\/p>\n\n\n\n \ub85c\ubd07 \uacf5\ud559 \uae30\uc220\uc758 \uc644\uc804 \uc790\ub3d9 \ud53c\ud3ab \uc6cc\ud06c \uc2a4\ud14c\uc774\uc158\uc758 \uad6c\uccb4 \uc608 <\/a><\/p>\n\n\n\n<\/figure>\n\n\n\n