{"id":8650,"date":"2025-02-25T17:58:35","date_gmt":"2025-02-25T09:58:35","guid":{"rendered":"https:\/\/opentrons.com.cn\/news\/\/"},"modified":"2025-02-25T17:58:56","modified_gmt":"2025-02-25T09:58:56","slug":"ngs-shiyanliucheng","status":"publish","type":"post","link":"https:\/\/opentrons.com.cn\/ko\/news\/ngs-shiyanliucheng\/","title":{"rendered":"NGS \uc2e4\ud5d8 \uacfc\uc815"},"content":{"rendered":"\n
\uc624\ub298\ub0a0 \uae30\uc220\uc758 \ube60\ub978 \uac1c\ubc1c\ub85c NGS (\ucc28\uc138\ub300 \uc2dc\ud000\uc2f1) \uae30\uc220\uc740 \ub192\uc740 \ucc98\ub9ac\ub7c9, \ub192\uc740 \ud6a8\uc728\uc131 \ubc0f \ub192\uc740 \uc815\ud655\ub3c4\ub85c \uc778\ud574 \uc0dd\uc758\ud559 \uc5f0\uad6c\uc5d0\uc11c \uc911\uc694\ud55c \uc704\uce58\ub97c \ucc28\uc9c0\ud588\uc2b5\ub2c8\ub2e4. \ucd5c\ucca8\ub2e8 \uc720\uc804\uc790 \uc2dc\ud000\uc2f1 \uae30\uc220\ub85c\uc11c NGS\ub294 \uc720\uc804\uccb4\ud559, \uc804 \uc0ac\uccb4 \ubc0f \uae30\ud0c0 \ubd84\uc57c\uc758 \ubc1c\ub2ec\uc744 \ud06c\uac8c \ucd09\uc9c4\ud588\uc744\ubfd0\ub9cc \uc544\ub2c8\ub77c \uc9c8\ubcd1\uc758 \uc9c4\ub2e8, \uce58\ub8cc \ubc0f \uc608\ubc29\uc5d0 \ub300\ud55c \uac15\ub825\ud55c \uc9c0\uc6d0\uc744 \uc81c\uacf5\ud588\uc2b5\ub2c8\ub2e4.<\/p>\n\n\n\n
<\/p>\n\n\n\n ngs \uc2e4\ud5d8 \ud504\ub85c\uc138\uc2a4 <\/a><\/p>\n\n\n\n 1. DNA \ucd94\ucd9c \ubc0f \ud488\uc9c8 \uac80\uc0ac <\/strong> 1. 2. DNA \ucd94\ucd9c : \uc0d8\ud50c\uc5d0\uc11c DNA\ub97c \ucd94\ucd9c\ud558\uae30 \uc704\ud574 \ud398\ub180 \ud074\ub85c\ub85c\ud3ec\ub984 \ucd94\ucd9c \ubc29\ubc95, \uc790\uae30 \ube44\ub4dc \ubc29\ubc95 \ub4f1\uacfc \uac19\uc740 \uc801\uc808\ud55c \ucd94\ucd9c \ubc29\ubc95\uc744 \uc0ac\uc6a9\ud569\ub2c8\ub2e4. 3. \ud488\uc9c8 \uac80\uc0ac : DNA\uc758 \ub18d\ub3c4, \uc21c\ub3c4 \ubc0f \ubb34\uacb0\uc131\uc740 \uc804\uae30 \uc601\ub3d9, \ubd84\uad11 \uad11\ub3c4\uacc4 \ub4f1\uc5d0 \uc758\ud574 \uac80\ucd9c\ub418\uc5b4 DNA \ud488\uc9c8\uc774 \ud6c4\uc18d \uc2e4\ud5d8 \uacb0\uacfc\uc758 \uc694\uad6c \uc0ac\ud56d\uc744 \ucda9\uc871\ud558\ub3c4\ub85d\ud569\ub2c8\ub2e4.<\/p>\n\n\n\n 2. \ub77c\uc774\ube0c\ub7ec\ub9ac \uad6c\uc131 <\/strong> 1. DNA\ub97c \uc2dc\ud000\uc11c \ud310\ub3c5\uc5d0 \uc801\ud569\ud55c \uae38\uc774 \uac04\uaca9\uc73c\ub85c \ub098\ub204\uace0 \ub9d0\uae30 \uc218\ub9ac, \uc870\uac01 \uc2a4\ud06c\ub9ac\ub2dd \ubc0f \uae30\ud0c0 \ucc98\ub9ac\ub97c \uc218\ud589\ud558\uc2ed\uc2dc\uc624. 2. \ub9c1\ucee4 \ucd94\uac00 : DNA \ub2e8\ud3b8\uc758 \uc591\ucabd \ub05d\uc5d0 \ud2b9\uc815 \ub9c1\ucee4 \uc2dc\ud000\uc2a4\ub97c \ucd94\uac00\ud569\ub2c8\ub2e4. 3. PCR \uc99d\ud3ed : \ub9c1\ucee4 \uc11c\uc5f4\uc744 \ud15c\ud50c\ub9bf\uc73c\ub85c \uc0ac\uc6a9\ud558\uc5ec, PCR \uc99d\ud3ed\uc5d0 \uc758\ud574 \ucda9\ubd84\ud55c \uc218\uc758 DNA \ub77c\uc774\ube0c\ub7ec\ub9ac\ub97c \uc5bb\uc5c8\ub2e4.<\/p>\n\n\n\n 3. \ub300\uc0c1 \ucea1\ucc98 (\uc120\ud0dd \uc0ac\ud56d) <\/strong> \ud2b9\uc815 \uc601\uc5ed (\uc608 : \uc804\uccb4 \uc5d1\uc19c \uc2dc\ud000\uc2f1, \uac8c\ub188-\ud2b9\uc774 \uc801 \uc601\uc5ed \uc2dc\ud000\uc2f1 \ub4f1)\uc758 \uc2dc\ud000\uc2f1 \ud504\ub85c\uc81d\ud2b8\uc5d0 \ub300\ud55c \ud45c\uc801 \ucea1\ucc98 \uae30\uc220\uc740 \ub77c\uc774\ube0c\ub7ec\ub9ac\ub85c\ubd80\ud130 \ubaa9\ud45c \uc2dc\ud000\uc2a4\ub97c \ud6a8\uc728\uc801\uc73c\ub85c \ud48d\ubd80\ud558\uac8c\ud558\uae30 \uc704\ud574 \ud544\uc694\ud558\ub2e4. \uc774 \ub2e8\uacc4\ub294 \uc2dc\ud000\uc2f1 \ub370\uc774\ud130\uc758 \uc815\ud655\uc131\uacfc \uae4a\uc774\ub97c \ud5a5\uc0c1\uc2dc\ucf1c \uc720\uc804\uc790 \ub610\ub294 \uad00\uc2ec \uc601\uc5ed\uc758 \ud45c\uc801\ud654 \ub41c \ubd84\uc11d\uc744 \ub3c4\uc640\uc90d\ub2c8\ub2e4.<\/p>\n\n\n\n iv. \uc628-\uc544\uc2e0 \uc2dc\ud000\uc2f1 <\/strong> 1 : \uc2dc\ud000\uc11c\uc758 \uc720\ub7c9 \uc140\uc5d0 \uc900\ube44\ud55c \ub77c\uc774\ube0c\ub7ec\ub9ac\ub97c\ub85c\ub4dc\ud558\uc5ec \ud6c4\uc18d \uc2dc\ud000\uc2f1\uc5d0 \ud544\uc694\ud55c \uc0d8\ud50c\uc744 \uc81c\uacf5\ud569\ub2c8\ub2e4. 2. \uc2dc\ud000\uc2f1 \ubc18\uc751 : \uc2dc\ud000\uc11c\ub294 \ud569\uc131, \uc2dc\ud000\uc2f1 \ub610\ub294 \uacb0\ucc30 \ubc0f \uc2dc\ud000\uc2f1 \ubc29\ubc95\uc744 \uc0ac\uc6a9\ud558\uc5ec \ub77c\uc774\ube0c\ub7ec\ub9ac\uc758 \ud575\uc0b0 \ub2e8\ud3b8\uc744 \uc2dc\ud000\uc2f1\ud569\ub2c8\ub2e4. \uc2dc\ud000\uc2f1 \uacf5\uc815 \ub3d9\uc548, \uc2dc\ud000\uc11c\ub294 \uc810\ucc28\uc801\uc73c\ub85c \ud615\uad11 \ud45c\uc9c0 \ub41c DNTP (Deoxyribonucleoside \ud2b8\ub9ac \ud3ec\uc2a4\ud398\uc774\ud2b8)\ub97c \ub3c4\uc785\ud558\uace0 \ud615\uad11 \uc2e0\ud638\ub97c \ubaa8\ub2c8\ud130\ub9c1\ud568\uc73c\ub85c\uc368 \uac01 \uc704\uce58\uc758 \uae30\ubcf8 \uc720\ud615\uc744 \uacb0\uc815\ud55c\ub2e4. 3. \uc774\ubbf8\uc9c0 \ucea1\ucc98 \ubc0f \ub370\uc774\ud130 \ubd84\uc11d : \uace0\ud574\uc0c1\ub3c4 \uce74\uba54\ub77c\ub97c \uc0ac\uc6a9\ud558\uc5ec \ud615\uad11 \uc2e0\ud638\uc758 \uc774\ubbf8\uc9c0\ub97c \ucea1\ucc98 \ud55c \ub2e4\uc74c \uc0dd\ubb3c \uc815\ubcf4\ud559 \uc18c\ud504\ud2b8\uc6e8\uc5b4\ub97c \ud1b5\ud574 \uc774\ubbf8\uc9c0 \ubc0f \uc2dc\ud000\uc2f1 \ub370\uc774\ud130\ub97c \ucc98\ub9ac\ud558\uace0 \ubd84\uc11d\ud558\uc5ec \uc644\uc804\ud55c \uc2dc\ud000\uc2f1 \uacb0\uacfc\ub97c \uc5bb\uc2b5\ub2c8\ub2e4.<\/p>\n\n\n\n 5. \ub370\uc774\ud130 \ubd84\uc11d <\/strong> 1. 2. 2 \ucc28 \ubd84\uc11d : \uae30\ubcf8 \ud488\uc9c8 \uc2a4\ucf54\uc5b4\ub9c1, \uc2dc\ud000\uc2a4 \uc815\ub82c, \ubcc0\ub3d9 \uac10\uc9c0 \ubc0f \uae30\ud0c0 \ub2e8\uacc4\ub97c \ud3ec\ud568\ud558\uc5ec \uc8fc\ub85c \uae30\uae30\uc5d0 \uc758\ud574 \uc790\ub3d9\uc73c\ub85c \uc644\ub8cc\ub429\ub2c8\ub2e4. 3. 3 \ub2e8\uacc4 \ubd84\uc11d : VCF \ud30c\uc77c\uc758 \ucc98\ub9ac\uc640 \uad00\ub828\uc774 \uc788\uc73c\uba70, \ucd5c\uc885 \uc2dc\ud000\uc2f1 \uacb0\uacfc \ubc0f \uc0dd\ubb3c\ud559\uc801 \uc911\uc694\uc131\uc744 \uc5bb\uae30 \uc704\ud574 \uc720\uc804\uc790 \uc8fc\uc11d, \ubcc0\ud615 \ud544\ud130\ub9c1 \ub4f1\uacfc \uac19\uc740 \uac1c\uc778\ud654 \ub41c \uc870\uc815 \uc77c \uc218 \uc788\uc2b5\ub2c8\ub2e4.<\/p>\n\n\n\n 6. \uacb0\uacfc \ud574\uc11d \ubc0f \ubcf4\uace0\uc11c <\/strong> \ub370\uc774\ud130 \ubd84\uc11d \uacb0\uacfc\ub97c \ubc14\ud0d5\uc73c\ub85c \ub2e8\uc77c \ub274\ud074\ub808\uc624\ud2f0\ub4dc \ub2e4\ud615\uc131 (SNP), \uc0bd\uc785 \ub610\ub294 \uc0ad\uc81c (Indel), \uad6c\uc870\uc801 \ubcc0\ud654 \ub4f1\uacfc \uac19\uc740 \uc2dc\ud000\uc2f1 \ub370\uc774\ud130\uc758 \ubcc0\ud615 \uc815\ubcf4\ub97c \ud574\uc11d\ud558\uc5ec \uad00\ub828 \ub370\uc774\ud130\ubca0\uc774\uc2a4 \ubc0f \ubb38\ud5cc \uc815\ubcf4\ub97c \uc870\ud569\ud558\uc5ec \uc124\uba85 \ubc0f \uc0dd\ubb3c\ud559\uc801 \uc911\uc694\uc131\uc5d0 \ub300\ud55c \ubcf4\uace0\uc11c\ub97c \uc81c\uacf5\ud569\ub2c8\ub2e4.<\/p>\n\n\n\n NGS \uc2e4\ud5d8 \ud504\ub85c\uc138\uc2a4\ub294 \ubcf5\uc7a1\ud558\uace0 \uc0c1\uc138\ud55c \ud504\ub85c\uc138\uc2a4\ub85c DNA \ucd94\ucd9c, \ub77c\uc774\ube0c\ub7ec\ub9ac \uad6c\uc131, \ub300\uc0c1 \ucea1\ucc98 (\uc120\ud0dd \uc0ac\ud56d), \ub370\uc774\ud130 \ubd84\uc11d\uc5d0 \ub300\ud55c \ucef4\ud4e8\ud130 \uc2dc\ud000\uc2f1 \ub4f1\uc758 \uc5ec\ub7ec \ub9c1\ud06c\ub97c \ub2e4\ub8e8\ub294 \ubcf5\uc7a1\ud558\uace0 \uc0c1\uc138\ud55c \ud504\ub85c\uc138\uc2a4\uc785\ub2c8\ub2e4. \uac01 \ub2e8\uacc4\ub294 \uc911\uc694\ud558\uba70 \uc2dc\ud000\uc2f1 \uacb0\uacfc\uc758 \uc815\ud655\uc131\uacfc \uc2e0\ub8b0\uc131\uc744 \ubcf4\uc7a5\ud558\uae30 \uc704\ud574 \uc5c4\uaca9\ud55c \uae30\uc220 \uc6b4\uc601 \ubc0f \ud488\uc9c8 \uad00\ub9ac\uac00 \ud544\uc694\ud569\ub2c8\ub2e4.<\/p>\n\n\n\n \uad00\ub828 \uc77d\uae30 \uad8c\uc7a5 \uc0ac\ud56d<\/p>\n\n\n\n opentrons \ud53c\ud3ab \uc5f4 \uc21c\ud658 \ubaa8\ub4c8 gen2 <\/a><\/p>\n\n\n\n \ud53c\ud3ab \ud305 \ub85c\ubd07 \uacc4\uae30 \uc11c\ube44\uc2a4\uc758 \uac1c\uc694 <\/a><\/p>\n\n\n\n \uc790\ub3d9 NGS \ub77c\uc774\ube0c\ub7ec\ub9ac \uc900\ube44 \uc804\uc790 \ucc45 <\/a><\/p>\n\n\n\n \ud3f4\ub9ac\uba38 \ub77c\uc81c \uc5f0\uc1c4 \ubc18\uc751\uc758 \uc6d0\ub9ac \ubc0f \ud504\ub85c\uc138\uc2a4 <\/a><\/p>\n\n\n\n<\/figure>\n\n\n\n