{"id":8527,"date":"2025-02-06T17:37:01","date_gmt":"2025-02-06T09:37:01","guid":{"rendered":"https:\/\/opentrons.com.cn\/news\/\/"},"modified":"2025-02-06T17:38:00","modified_gmt":"2025-02-06T09:38:00","slug":"pcr-yinwushejibuzhou","status":"publish","type":"post","link":"https:\/\/opentrons.com.cn\/ko\/news\/pcr-yinwushejibuzhou\/","title":{"rendered":"PCR \ud504\ub77c\uc774\uba38 \uc124\uacc4\ub97c\uc704\ud55c \uc0c1\uc138\ud55c \ub2e8\uacc4"},"content":{"rendered":"\n
PCR (\uc911\ud569 \ud6a8\uc18c \uc5f0\uc1c4 \ubc18\uc751)\uc740 \ubd84\uc790 \uc0dd\ubb3c\ud559\uc5d0\uc11c \uc77c\ubc18\uc801\uc73c\ub85c \uc0ac\uc6a9\ub418\ub294 \uae30\uc220 \uc911 \ud558\ub098\uc774\uba70 \uc720\uc804\uc790 \uc99d\ud3ed, \uc720\uc804\uc790 \ub3cc\uc5f0\ubcc0\uc774 \uac80\ucd9c \ubc0f \uc720\uc804\uc790 \ud074\ub85c\ub2dd\uacfc \uac19\uc740 \uc5f0\uad6c \ubd84\uc57c\uc5d0\uc11c \ub110\ub9ac \uc0ac\uc6a9\ub429\ub2c8\ub2e4. PCR \uc2e4\ud5d8\uc5d0\uc11c \ud504\ub77c\uc774\uba38\uc758 \ud488\uc9c8\uc740 \uc99d\ud3ed\uc758 \ud2b9\uc774\uc131, \ud6a8\uc728 \ubc0f \uc815\ud655\ub3c4\uc5d0 \uc9c1\uc811 \uc601\ud5a5\uc744 \ubbf8\uce58\uae30 \ub54c\ubb38\uc5d0 \uc62c\ubc14\ub978 \ud504\ub77c\uc774\uba38 \uc124\uacc4\uac00 \uc911\uc694\ud569\ub2c8\ub2e4.<\/p>\n\n\n\n PCR \ud504\ub77c\uc774\uba38 \uc124\uacc4 <\/a><\/p>\n\n\n\n 1. \ud45c\uc801 \uc720\uc804\uc790 \uc601\uc5ed\uc758 \ud504\ub77c\uc774\uba38 \uc124\uacc4\ub97c \uacb0\uc815\ud558\ub294 \uccab \ubc88\uc9f8 \ub2e8\uacc4\ub294 PCR \uc99d\ud3ed\uc758 \ud45c\uc801 \uc601\uc5ed\uc744 \uba85\ud655\ud788\ud558\ub294 \uac83\uc774\ub2e4. \ud504\ub77c\uc774\uba38\ub97c \uc124\uacc4 \ud560 \ub54c \ubd88\ud544\uc694\ud55c \ub3cc\uc5f0\ubcc0\uc774 \ub610\ub294 \uc624\ub958\uac00 \ub3c4\uc785\ub418\uc9c0 \uc54a\ub3c4\ub85d \uc54c\ub824\uc9c4 \uc720\uc804\uc790 \uc11c\uc5f4\uc5d0 \uae30\ucd08\ud558\uc5ec \uad00\uc2ec \uc601\uc5ed\uc744 \uc120\ud0dd\ud574\uc57c\ud55c\ub2e4. \ubaa9\ud45c \uc601\uc5ed\uc758 \uae38\uc774\ub294 \uc77c\ubc18\uc801\uc73c\ub85c 100 ~ 1000 \uae30\ubcf8 \uc30d\uc774\uba70 \uc99d\ud3ed \ud6a8\uacfc\uc5d0 \uc601\ud5a5\uc744 \ubbf8\uce69\ub2c8\ub2e4.<\/p>\n\n\n\n 2. \ud504\ub77c\uc774\uba38 \uae38\uc774\uc758 \uc120\ud0dd PCR \ud504\ub77c\uc774\uba38\uc758 \uae38\uc774\ub294 \uc77c\ubc18\uc801\uc73c\ub85c 18-24 \uae30\ubcf8 \uc30d\uc774\ub2e4. \uc774\ub294 \ub108\ubb34 \uc9e7\uc740 \ud504\ub77c\uc774\uba38\uac00 \ube44\ud2b9\uc774\uc801 \uacb0\ud569\uc744 \uc720\ubc1c\ud558\uc5ec \uc99d\ud3ed\uc758 \ud2b9\uc774\uc131\uc5d0 \uc601\ud5a5\uc744 \ubbf8\uce58\ub294 \ubc18\uba74, \ub108\ubb34 \uae34 \ud504\ub77c\uc774\uba38\ub294 \ud504\ub77c\uc774\uba38\uc758 \uc6a9\uc735 \uc628\ub3c4 (TM \uac12)\uc5d0 \uc601\ud5a5\uc744 \uc904 \uc218 \uc788\uae30 \ub54c\ubb38\uc5d0 \uc99d\ud3ed \ud6a8\uc728\uc5d0 \uc601\ud5a5\uc744 \uc904 \uc218 \uc788\uc2b5\ub2c8\ub2e4. \uc77c\ubc18\uc801\uc73c\ub85c, \uc57d 20 \uac1c\uc758\ubca0\uc774\uc2a4 \uc30d\uc758 \uae38\uc774\ub97c \uc120\ud0dd\ud558\ub294 \uac83\uc774 \ub354 \uc801\uc808\ud569\ub2c8\ub2e4.<\/p>\n\n\n\n 3. \ud504\ub77c\uc774\uba38\uc758 \uc6a9\uc735 \uc628\ub3c4 (TM \uac12)\ub294 \ud504\ub77c\uc774\uba38\uac00 \ud15c\ud50c\ub9bf DNA\uc5d0 \uacb0\ud569 \ud560 \ub54c \uc911\uc694\ud55c \ub9e4\uac1c \ubcc0\uc218\uc774\uba70, \ud504\ub77c\uc774\uba38 \ubc0f \uc8fc\ud615 DNA\uc758 \uacb0\ud569 \uac15\ub3c4\uc5d0 \uc9c1\uc811 \uc601\ud5a5\uc744 \ubbf8\uce69\ub2c8\ub2e4. \ud504\ub77c\uc774\uba38\uc758 TM \uac12\uc740 \uc77c\ubc18\uc801\uc73c\ub85c 55 \u00b0 C\uc5d0\uc11c 65 \u00b0 C \uc0ac\uc774\uc785\ub2c8\ub2e4. \ud504\ub77c\uc774\uba38 \uc30d\uc758 TM \uac12\uc740 \ub3d9\uc77c\ud55c PCR \ubc18\uc751 \uc870\uac74 \ud558\uc5d0\uc11c \ub450 \ud504\ub77c\uc774\uba38\uac00 \ud6a8\uacfc\uc801\uc73c\ub85c \uacb0\ud569 \ud560 \uc218 \uc788\ub3c4\ub85d \uac00\ub2a5\ud55c \ud55c \uac00\uae5d\uae30 \ub54c\ubb38\uc5d0 \uac00\ub2a5\ud55c \ud55c \uac00\uae5d\ub2e4. \ud504\ub77c\uc774\uba38\uc758 TM \uac12\uc744 \uacc4\uc0b0\ud558\uae30\uc704\ud55c \uacf5\uc2dd\uc740 \ub2e4\uc74c\uacfc \uac19\uc2b5\ub2c8\ub2e4. $ tm = 2 (a+t)+4 (g+c) $ \uc5ec\uae30\uc11c A, T, G \ubc0f C\ub294 \uac01\uac01 \ud504\ub77c\uc774\uba38\uc758 \uc0c1\uc751\ud558\ub294 \uc5fc\uae30 \uc218\uc785\ub2c8\ub2e4. \uacc4\uc0b0 \uc911\uc5d0 \ud504\ub77c\uc774\uba38\ub4e4 \uc0ac\uc774\uc758 TM \ucc28\uc774\uac00 5 \u00b0 C\ub97c \ucd08\uacfc\ud558\uc9c0 \uc54a\ub3c4\ub85d\ud574\uc57c\ud569\ub2c8\ub2e4.<\/p>\n\n\n\n 4. \ud504\ub77c\uc774\uba38\uc758 GC \ud568\ub7c9\uc740 \ud504\ub77c\uc774\uba38\uc758 \uc548\uc815\uc131\uc5d0 \uc911\uc694\ud55c \uc601\ud5a5\uc744 \ubbf8\uce69\ub2c8\ub2e4. \ud504\ub77c\uc774\uba38\uc758 GC \ud568\ub7c9\uc740 \uc77c\ubc18\uc801\uc73c\ub85c 40%\uc5d0\uc11c 60% \uc0ac\uc774\uc5d0 \uad8c\uc7a5\ub429\ub2c8\ub2e4. GC \ud568\ub7c9\uc774 \uc9c0\ub098\uce58\uac8c \ub192\uac70\ub098 \ub0ae\uc740 GC \ud568\ub7c9\uc740 \ud504\ub77c\uc774\uba38\uc758 \uc548\uc815\uc131\uc5d0 \uc601\ud5a5\uc744 \ubbf8\uccd0 PCR \ubc18\uc751\uc758 \ud6a8\uc728\uacfc \ud2b9\uc774\uc131\uc5d0 \uc601\ud5a5\uc744 \ubbf8\uce69\ub2c8\ub2e4. GC \ud568\ub7c9\uc774 \ub192\uc740 \ud504\ub77c\uc774\uba38\ub294 \uc99d\ud3ed \uc0dd\uc131\ubb3c\uc758 \ube44\ud2b9\uc774\uc801 \uc99d\ud3ed\uc744 \uc720\ubc1c\ud560 \uc218\uc788\ub294 \ubc18\uba74, GC \ud568\ub7c9\uc774 \ub0ae\uc740 \ud504\ub77c\uc774\uba38\ub294 \ud504\ub77c\uc774\uba38\uc758 \uc57d\ud55c \uacb0\ud569 \uc6a9\ub7c9\uc744 \uc720\ubc1c\ud558\uc5ec \uc99d\ud3ed \ud6a8\uacfc\uc5d0 \uc601\ud5a5\uc744 \uc904 \uc218\uc788\ub2e4.<\/p>\n\n\n\n 5. \ud504\ub77c\uc774\uba38\uc758 \uc2dc\ud000\uc2a4 \ud2b9\uc131 \ud504\ub77c\uc774\uba38\ub97c \uc124\uacc4 \ud560 \ub54c \ub2e4\uc74c\uacfc \uac19\uc740 \uc0c1\ud669\uc744 \ud53c\ud574\uc57c\ud569\ub2c8\ub2e4. 1. \uc774\ub7c9 \uccb4 \ubc0f \ud5e4\uc5b4\ud540 \uad6c\uc870 : \ud504\ub77c\uc774\uba38 \uc0ac\uc774\uc758 \uc774\ub7c9 \uccb4 \ubc0f \ud5e4\uc5b4\ud540 \uad6c\uc870\ub294 PCR\uc758 \uc815\uc0c1\uc801\uc778 \uc9c4\ud589\uc5d0 \uc601\ud5a5\uc744 \ubbf8\uce69\ub2c8\ub2e4. Oligocalc\uc640 \uac19\uc740 \uc628\ub77c\uc778 \ub3c4\uad6c\ub97c \uc0ac\uc6a9\ud558\uc5ec \uc2e4\ud5d8 \uacb0\uacfc\uc5d0 \uc601\ud5a5\uc744 \ubbf8\uce58\uc9c0 \uc54a\ub3c4\ub85d \ud504\ub77c\uc774\uba38 \uc11c\uc5f4\uc5d0\uc11c \uc774\ub7c9 \uccb4 \ubc0f \ud5e4\uc5b4\ud540 \uad6c\uc870\ub97c \uac10\uc9c0\ud558\uc2ed\uc2dc\uc624. 2. \ubc18\ubcf5 \uc21c\uc11c : \ud504\ub77c\uc774\uba38 \uc2dc\ud000\uc2a4 (\uc608 : A \ub610\ub294 T\uc758 \uc5f0\uc18d \ubc18\ubcf5)\uc5d0\uc11c \ub108\ubb34 \ub9ce\uc740 \uc5f0\uc18d \ub3d9\uc77c\ud55c \uc5fc\uae30\ub97c \ud53c\ud558\uc2ed\uc2dc\uc624.\uc774 \uad6c\uc870\ub294 \ube44\ud2b9\uc774\uc801 \uacb0\ud569\uc744 \uc720\ubc1c\ud558\uae30 \uc27d\uc2b5\ub2c8\ub2e4. 3. \ubcf4\uc644 \uc2dc\ud000\uc2a4\ub97c \ud53c\ud558\uc2ed\uc2dc\uc624 : \uc124\uacc4\ub41c \ub450 \ud504\ub77c\uc774\uba38 \uc0ac\uc774\uc5d0 \uc0c1\ubcf4\uc801\uc778 \uc11c\uc5f4\uc774 \uc5c6\uc5b4\uc57c\ud569\ub2c8\ub2e4. \uadf8\ub807\uc9c0 \uc54a\uc73c\uba74 \ud504\ub77c\uc774\uba38 \uc774\ub7c9 \uccb4\uc758 \ud615\uc131\uc73c\ub85c \uc774\uc5b4\uc9c0\uace0 \uc99d\ud3ed \ud6a8\uc728\uc5d0 \uc601\ud5a5\uc744 \uc904 \uc218 \uc788\uc2b5\ub2c8\ub2e4.<\/p>\n\n\n\n 6. \ud504\ub77c\uc774\uba38\uc758 \ubc29\ud5a5 PCR \uc99d\ud3ed\uc740 \ub450 \ubc29\ud5a5\uc73c\ub85c \uc218\ud589\ub418\ubbc0\ub85c, \uac01\uac01 \ud45c\uc801 DNA\uc758 \uc804\ubc29 \ubc0f \uc5ed\ubc29\ud5a5 \uac00\ub2e5\uc5d0 \ud574\ub2f9\ud558\ub294 \ud55c \uc30d\uc758 \ud504\ub77c\uc774\uba38\ub97c \uc124\uacc4\ud574\uc57c\ud55c\ub2e4. \uc804\ubc29 \ud504\ub77c\uc774\uba38\ub294 \ud45c\uc801 DNA\uc758 \uc591\uc131 \uac00\ub2e5\uc5d0 \uacb0\ud569\ud558\ub294 \ubc18\uba74, \uc5ed \ud504\ub77c\uc774\uba38\ub294 \ud45c\uc801 DNA\uc758 \uc5ed \uac00\ub2e5\uc5d0 \uacb0\ud569\ud55c\ub2e4. \uc99d\ud3ed\uc758 \ud2b9\uc774\uc131\uc744 \ubcf4\uc7a5\ud558\uae30 \uc704\ud574, \uc815\ubc29\ud5a5 \ubc0f \uc5ed \ud504\ub77c\uc774\uba38\ub294 \uc778\uc811\ud55c \uc601\uc5ed\uacfc \ubcf4\uc644 \ubc29\ud5a5\uc73c\ub85c \uc124\uacc4\ub418\uc5b4\uc57c\ud569\ub2c8\ub2e4.<\/p>\n\n\n\n 7. \ud504\ub77c\uc774\uba38 \ub514\uc790\uc778 \ub3c4\uad6c\ub97c \uc0ac\uc6a9\ud558\uc5ec \uc624\ub298\ub0a0 \ub9ce\uc740 \ud504\ub77c\uc774\uba38 \ub514\uc790\uc778 \uc18c\ud504\ud2b8\uc6e8\uc5b4\uc640 \uc628\ub77c\uc778 \ub3c4\uad6c\ub97c \uc0ac\uc6a9\ud558\uc5ec \uacfc\ud559 \uc5f0\uad6c\uc6d0\ub4e4\uc774 Primer3, Primer-Blast \ub4f1\uacfc \uac19\uc740 \ud504\ub77c\uc774\uba38\ub97c \ube60\ub974\uace0 \uc815\ud655\ud558\uac8c \ub514\uc790\uc778\ud558\ub294 \ub370 \ub3c4\uc6c0\uc774 \ub420 \uc218 \uc788\uc2b5\ub2c8\ub2e4. \uc774\ub7ec\ud55c \ub3c4\uad6c\ub294 \ud504\ub77c\uc774\uba38\uc758 TM \uac12, GC \ud568\ub7c9, \uc774\ub7c9 \uccb4 \ubc0f \ud5e4\uc5b4\ud540 \uad6c\uc870\ub97c \uc790\ub3d9\uc73c\ub85c \uacc4\uc0b0\ud558\uc5ec \ud504\ub77c\uc774\uba38 \uc124\uacc4 \ud504\ub85c\uc138\uc2a4\ub97c \ud06c\uac8c \ub2e8\uc21c\ud654 \ud560 \uc218 \uc788\uc2b5\ub2c8\ub2e4.<\/p>\n\n\n\n 8. \ud504\ub77c\uc774\uba38 \uac80\uc99d \uc124\uacc4\uac00 \uc644\ub8cc\ub41c \ud6c4, \ud504\ub77c\uc774\uba38\ub294 \ud45c\uc801 \uc11c\uc5f4\uc5d0 \ud2b9\uc774 \uc801\uc73c\ub85c \uacb0\ud569\ud558\uace0 \uc99d\ud3ed \ud6a8\uc728\uc774 \ub192\uc740\uc9c0 \ud655\uc778\ud558\uae30 \uc704\ud574 \uac80\uc99d\ub418\uc5b4\uc57c\ud55c\ub2e4. \uc77c\ubc18\uc801\uc73c\ub85c \uc0ac\uc6a9\ub418\ub294 \uac80\uc99d \ubc29\ubc95\uc740 PCR \ubc18\uc751\uc744 \ud1b5\ud574 \ud504\ub77c\uc774\uba38\uc758 \uc99d\ud3ed \ud6a8\uacfc\ub97c \ud14c\uc2a4\ud2b8\ud558\uace0 \uc99d\ud3ed \uc0dd\uc131\ubb3c\uc758 \ud2b9\uc774\uc131\uc744 \ubd84\uc11d\ud558\ub294 \uac83\uc785\ub2c8\ub2e4. \uc99d\ud3ed \uc81c\ud488\uc774 \uc608\uc0c1 \ud06c\uae30\ub97c \ucda9\uc871\ud558\uace0 \ube44\ud2b9\uc774\uc801 \ub300\uc5ed\uc774 \uc544\ub2cc \uacbd\uc6b0 \ud504\ub77c\uc774\uba38 \uc124\uacc4\uac00 \uc131\uacf5\ud588\uc74c\uc744 \ub098\ud0c0\ub0c5\ub2c8\ub2e4.<\/p>\n\n\n\n PCR \ud504\ub77c\uc774\uba38 \uc124\uacc4\ub294 \ubd84\uc790 \uc0dd\ubb3c\ud559 \uc2e4\ud5d8\uc758 \uc8fc\uc694 \ub2e8\uacc4 \uc911 \ud558\ub098\uc774\uba70 \uc2e4\ud5d8\uc758 \uc131\uacf5\uc5d0 \uc601\ud5a5\uc744 \ubbf8\uce69\ub2c8\ub2e4. \ud504\ub77c\uc774\uba38 \uc2dc\ud000\uc2a4, \uae38\uc774, GC \ucee8\ud150\uce20, TM \uac12 \ubc0f \uae30\ud0c0 \ub9e4\uac1c \ubcc0\uc218\ub97c \ud569\ub9ac\uc801\uc73c\ub85c \uc124\uacc4\ud558\uace0 \uae30\uc874 \uc124\uacc4 \ub3c4\uad6c \ubc0f \uac80\uc99d \ubc29\ubc95\uc744 \uc0ac\uc6a9\ud558\uc5ec PCR \uc2e4\ud5d8\uc758 \uc815\ud655\uc131\uacfc \ud6a8\uc728\uc131\uc744 \ud06c\uac8c \ud5a5\uc0c1\uc2dc\ud0ac \uc218 \uc788\uc2b5\ub2c8\ub2e4. \uc2e4\uc81c \uc791\ub3d9\uc5d0\uc11c \ud504\ub77c\uc774\uba38 \uc124\uacc4\ub294 \ucd5c\uc0c1\uc758 \uc99d\ud3ed \ud6a8\uacfc\ub97c \ubcf4\uc7a5\ud558\uae30 \uc704\ud574 \uc2e4\ud5d8\uc758 \ud2b9\uc815 \uc694\uad6c\uc5d0 \ub530\ub77c \uc9c0\uc18d\uc801\uc73c\ub85c \uc870\uc815 \ubc0f \ucd5c\uc801\ud654\ub418\uc5b4\uc57c\ud569\ub2c8\ub2e4.<\/p>\n\n\n\n \uad00\ub828 \uc77d\uae30 \uad8c\uc7a5 \uc0ac\ud56d<\/p>\n\n\n\n SARS-cov-2\uc758 \uc911\ud654 \ud56d\uccb4\ub97c \uc790\ub3d9\ud654\ud558\ub824\uba74 \ud788\ud130 \uc250\uc774\ud0b9 \ubaa8\ub4c8\uc744 \uc0ac\uc6a9\ud558\uc2ed\uc2dc\uc624.<\/a><\/p>\n\n\n\n opentrons flex \uc790\ub3d9\ud654 \ud53c\ud3ab \uc6cc\ud06c \uc2a4\ud14c\uc774\uc158 24 \uc2dc\uac04 \ub0b4\uc5d0 \ucd5c\ub300 384 \uac1c\uc758 \ud50c\ub77c\uc2a4\ubbf8\ub4dc \ub77c\uc774\ube0c\ub7ec\ub9ac\ub97c \uc900\ube44 \ud560 \uc218 \uc788\uc2b5\ub2c8\ub2e4 <\/a><\/p>\n\n\n\n 3D \uc778\uc1c4 \ub514\ub809\ud1a0\ub9ac <\/a><\/p>\n\n\n\n opentrons ot-2 PCR \uc6cc\ud06c \uc2a4\ud14c\uc774\uc158 \uc628\ub3c4 \uc81c\uc5b4 \ubaa8\ub4c8 \uad50\uc815 <\/a><\/p>\n\n\n\n<\/figure>\n\n\n\n