{"id":8517,"date":"2025-01-19T18:18:54","date_gmt":"2025-01-19T10:18:54","guid":{"rendered":"https:\/\/opentrons.com.cn\/news\/\/"},"modified":"2025-01-19T18:19:26","modified_gmt":"2025-01-19T10:19:26","slug":"pcr-yinwushejiyuanli","status":"publish","type":"post","link":"https:\/\/opentrons.com.cn\/ko\/news\/pcr-yinwushejiyuanli\/","title":{"rendered":"PCR \ud504\ub77c\uc774\uba38\uc758 \uc124\uacc4 \uc6d0\ub9ac"},"content":{"rendered":"\n
\ubd84\uc790 \uc0dd\ubb3c\ud559 \uc5f0\uad6c\uc5d0\uc11c PCR (\uc911\ud569 \ud6a8\uc18c \uc5f0\uc1c4 \ubc18\uc751) \uae30\uc220\uc740 \uac00\uc7a5 \uc77c\ubc18\uc801\uc73c\ub85c \uc0ac\uc6a9\ub418\ub294 \uc911\uc694\ud55c \ub3c4\uad6c \uc911 \ud558\ub098\uc774\uba70 PCR \ud504\ub77c\uc774\uba38\uc758 \uc124\uacc4\ub294 PCR \uc2e4\ud5d8\uc758 \uc131\uacf5\uc744 \ubcf4\uc7a5\ud558\uae30\uc704\ud55c \uc8fc\uc694 \uc694\uc778 \uc911 \ud558\ub098\uc785\ub2c8\ub2e4. PCR \ud504\ub77c\uc774\uba38\uc758 \uc124\uacc4 \uc6d0\ub9ac\ub294 \uc2e4\ud5d8\uc758 \uc815\ud655\uc131, \ud2b9\uc774\uc131 \ubc0f \uc99d\ud3ed \ud6a8\uc728\uacfc \uc9c1\uc811 \uad00\ub828\uc774 \uc788\uc73c\uba70, \ud504\ub77c\uc774\uba38 \uc124\uacc4\uc758 \uae30\ubcf8 \uc6d0\ub9ac\ub97c \uc774\ud574\ud558\uace0 \ub9c8\uc2a4\ud130\ud558\ub294 \uac83\uc740 \uacfc\ud559\uc801 \uc5f0\uad6c\uc5d0 \uc911\uc694\ud569\ub2c8\ub2e4.<\/p>\n\n\n\n pcr primers <\/a><\/p>\n\n\n\n 1. PCR \ud504\ub77c\uc774\uba38\uc758 \uae30\ubcf8 \uac1c\ub150 PCR \ud504\ub77c\uc774\uba38\ub294 \uc9e7\uc740 DNA \ub610\ub294 RNA \ub2e8\ud3b8\uc774\uba70, \uc77c\ubc18\uc801\uc73c\ub85c \uae38\uc774\uac00 18-30 \uc5fc\uae30 \uc0ac\uc774\uc785\ub2c8\ub2e4. \uadf8\ub4e4\uc740 PCR \ubc18\uc751\uc5d0\uc11c \ub9e4\uc6b0 \uc911\uc694\ud55c \uc5ed\ud560\uc744\ud558\uba70, \uc8fc\ud615 DNA\uc758 \uc591\ucabd \ub05d\uc5d0\uc11c \uad6c\uccb4\uc801\uc73c\ub85c \uacb0\ud569\ud558\uace0 DNA \ud3f4\ub9ac\uba38 \ub77c\uc81c\uc5d0 \ub300\ud55c \uac1c\uc2dc \uc810\uc744 \uc81c\uacf5 \ud560 \uc218\uc788\ub2e4. \uac04\ub2e8\ud788 \ub9d0\ud574, \ud504\ub77c\uc774\uba38\ub294 PCR \uc2e4\ud5d8\uc758 \"\uc2dc\uc791\uc810 \ub9c8\ucee4\"\uc640 \uac19\uc73c\uba70, \uc774\ub294 \uc911\ud569 \ud6a8\uc18c\uac00 \uc62c\ubc14\ub978 \uc704\uce58\uc5d0\uc11c \uc99d\ud3ed\ub418\uae30 \uc2dc\uc791\ud569\ub2c8\ub2e4.<\/p>\n\n\n\n 2. PCR \ud504\ub77c\uc774\uba38 \uc124\uacc4\uc758 \uae30\ubcf8 \uc6d0\ub9ac 1. \ud504\ub77c\uc774\uba38 \uae38\uc774\uc758 \uc120\ud0dd \ud504\ub77c\uc774\uba38 \uae38\uc774\ub294 PCR \uc2e4\ud5d8\uc758 \uc131\uacf5\uc5d0 \uc601\ud5a5\uc744 \ubbf8\uce58\ub294 \ud575\uc2ec \uc694\uc18c\uc785\ub2c8\ub2e4. \uc77c\ubc18\uc801\uc73c\ub85c \ud504\ub77c\uc774\uba38\ub294 18 ~ 30 \uac1c\uc758 \uae38\uc774\uac00 \ub108\ubb34 \uc9e7\uc544\uc11c \uc99d\ud3ed \ud2b9\uc774\uc131\uc774 \uc88b\uc9c0 \uc54a\uc744 \uc218 \uc788\uc73c\uba70, \ube44\ud2b9\uc774\uc801 \uc99d\ud3ed\uc744 \uc720\ubc1c\ud558\uac70\ub098 \ud504\ub77c\uc774\uba38\uc758\uc790\uac00 \ubc14\uc778\ub529\uc744 \uc99d\uac00\uc2dc\ud0ac \uc218 \uc788\uc2b5\ub2c8\ub2e4. \ud504\ub77c\uc774\uba38\uc758 \uae38\uc774\ub294 \uc77c\ubc18\uc801\uc73c\ub85c \ud45c\uc801 \ub2e8\ud3b8\uc758 \uae38\uc774 \ubc0f \uc2e4\ud5d8 \uc694\uad6c \uc0ac\ud56d\uc5d0 \ub530\ub77c \uc870\uc815\ub429\ub2c8\ub2e4. \ud504\ub77c\uc774\uba38\uc758 GC \ud568\ub7c9\uc740 \ud504\ub77c\uc774\uba38\uc5d0\uc11c G (Guanine) \ubc0f C (Cytosine)\uc758 \ube44\uc728\uc744 \ub098\ud0c0\ub0c5\ub2c8\ub2e4. \uc774\uc0c1\uc801\uc778 PCR \ud504\ub77c\uc774\uba38 GC \ud568\ub7c9\uc740 40%\uc5d0\uc11c 60% \uc0ac\uc774 \uc5ec\uc57c\ud569\ub2c8\ub2e4. \uc801\uc808\ud55c GC \ud568\ub7c9\uc740 \ub192\uc740 \uc548\uc815\uc131\uc744 \uc81c\uacf5\ud558\uba74\uc11c \ud45c\uc801 DNA\uc5d0 \ud504\ub77c\uc774\uba38\ub97c \uacb0\ud569\ud558\ub294 \ub370 \ub3c4\uc6c0\uc774\ub429\ub2c8\ub2e4. GC \ud568\ub7c9\uc774 \ub108\ubb34 \ub192\uac70\ub098 \ub108\ubb34 \ub0ae \uc73c\uba74 \ud45c\uc801 DNA\uc5d0 \ud504\ub77c\uc774\uba38\ub97c \ubd88\uc548\uc815\ud55c \uacb0\ud569\uc73c\ub85c \uc774\uc5b4\uc9c8 \uc218 \uc788\uc73c\uba70, \uc774\ub294 PCR \uc99d\ud3ed \ud6a8\uc728\uc5d0 \uc601\ud5a5\uc744 \ubbf8\uce69\ub2c8\ub2e4. 3. \ud504\ub77c\uc774\uba38\uc758 \uc5b4\ub2d0\ub9c1 \uc628\ub3c4 (TM) \ud504\ub77c\uc774\uba38\uc758 \uc5b4\ub2d0\ub9c1 \uc628\ub3c4 (TM)\ub294 \ud504\ub77c\uc774\uba38\uac00 \ud2b9\uc815 \uc628\ub3c4\uc5d0\uc11c \uc8fc\ud615 DNA\uc5d0 \uacb0\ud569\ud558\ub294 \uc628\ub3c4\ub97c \ub098\ud0c0\ub0c5\ub2c8\ub2e4. PCR \ubc18\uc751\uc758 \ud6a8\uacfc\ub97c \ubcf4\uc7a5\ud558\uae30 \uc704\ud574, \ud504\ub77c\uc774\uba38\uc758 TM \uac12\uc740 \uc720\uc0ac\ud574\uc57c\ud558\uba70, \ubc14\ub78c\uc9c1\ud558\uac8c\ub294 \ucc28\uc774\uac00 2 \u2103\ub97c \ucd08\uacfc\ud574\uc11c\ub294 \uc548\ub41c\ub2e4. \uc774\uc0c1\uc801\uc778 TM \uac12\uc740 \uc77c\ubc18\uc801\uc73c\ub85c 50 \u00b0 C\uc5d0\uc11c 65 \u00b0 C \uc0ac\uc774\uc785\ub2c8\ub2e4. \ud504\ub77c\uc774\uba38\uc758 TM \uac12\uc758 \ucc28\uc774\uac00 \ub108\ubb34 \ud06c\uba74 \ube44\ud6a8\uc728\uc801 \uc778 \uc99d\ud3ed \ubc0f \uace0\uc7a5\uc73c\ub85c \uc774\uc5b4\uc9c8 \uac83\uc785\ub2c8\ub2e4. 4. \ud504\ub77c\uc774\uba38 \uc774\ub7c9 \uccb4 \ubc0f \ud5e4\uc5b4\ud540 \uad6c\uc870\ub97c \ud53c\ud558\uc2ed\uc2dc\uc624. \uc774\ub7c9 \uccb4\ub294 2 \uac1c\uc758 \ud504\ub77c\uc774\uba38\uac00 \uc11c\ub85c \uacb0\ud569\ud558\uc5ec \ubc14\ub78c\uc9c1\ud558\uc9c0 \uc54a\uc740 2 \ucc28 \uad6c\uc870\ub97c \ud615\uc131 \ud560 \ub54c\uc774\uba70, \uc774\ub294 \uc99d\ud3ed\uc758 \ud2b9\uc774\uc131 \ubc0f \ud6a8\uc728\uc5d0 \uc601\ud5a5\uc744 \ubbf8\uce69\ub2c8\ub2e4. \ub610\ud55c, \ud504\ub77c\uc774\uba38\ub294 \ub610\ud55c \ud5e4\uc5b4\ud540 \uad6c\uc870\ub97c \ud615\uc131\ud558\uae30 \uc704\ud574 \ud53c\ud574\uc57c\ud558\uba70, \uc774\ub294 \ud504\ub77c\uc774\uba38\uc758 \uacb0\ud569 \ud6a8\uc728\uc744 \uac10\uc18c\uc2dc\ud0ac \uac83\uc774\ub2e4. \ub530\ub77c\uc11c \ud504\ub77c\uc774\uba38\ub97c \uc124\uacc4 \ud560 \ub54c \uc774\ub7ec\ud55c \ubc14\ub78c\uc9c1\ud558\uc9c0 \uc54a\uc740 \uad6c\uc870\uc758 \ud615\uc131\uc744 \uc608\uce21\ud558\uace0 \ud53c\ud558\uae30 \uc704\ud574 \uc801\uc808\ud55c \uacc4\uc0b0 \ub3c4\uad6c\ub97c \uc0ac\uc6a9\ud558\ub294 \uac83\uc774 \uc911\uc694\ud569\ub2c8\ub2e4. 5. \ud504\ub77c\uc774\uba38\uc758 \ud2b9\uc815 \uc124\uacc4. \ud504\ub77c\uc774\uba38\uc758 \ud2b9\uc774\uc131\uc740 PCR\uc758 \uc131\uacf5\uc5d0 \uc911\uc694\ud558\ub2e4. \ud504\ub77c\uc774\uba38\ub97c \uc124\uacc4 \ud560 \ub54c \ud504\ub77c\uc774\uba38\uac00 \ud45c\uc801 DNA\uc758 \ud2b9\uc815 \uc601\uc5ed\uc5d0 \uc815\ud655\ud558\uac8c \uacb0\ud569 \ud560 \uc218 \uc788\ub3c4\ub85d\ud574\uc57c\ud569\ub2c8\ub2e4. \ube44\ud2b9\uc774\uc801 \uc99d\ud3ed\uc744 \ud53c\ud558\uae30 \uc704\ud574, \ud504\ub77c\uc774\uba38 \uc11c\uc5f4\uc740 \ube44 \ud45c\uc801 \uc11c\uc5f4\uc5d0 \uacb0\ud569\ud558\ub294\uc9c0 \uc5ec\ubd80\ub97c \uc124\uacc4 \uc2dc\uac04\uc5d0 \uc810\uac80\ud574\uc57c\ud55c\ub2e4. \uc774\ub294 \uc77c\ubc18\uc801\uc73c\ub85c \ud504\ub77c\uc774\uba38 \ud2b9\uc774\uc131\uc744 \ubcf4\uc7a5\ud558\uae30 \uc704\ud574 Blast\uc640 \uac19\uc740 \uc0dd\ubb3c \uc815\ubcf4\ud559 \ub3c4\uad6c\ub97c \uc0ac\uc6a9\ud55c \uc11c\uc5f4 \uc815\ub82c \ubd84\uc11d\uc774 \ud544\uc694\ud569\ub2c8\ub2e4.<\/p>\n\n\n\n 2. PCR \ud504\ub77c\uc774\uba38 \uc124\uacc4\ub97c\uc704\ud55c \ub3c4\uad6c \ubc0f \uc18c\ud504\ud2b8\uc6e8\uc5b4 \uc0dd\ubb3c \uc815\ubcf4\ud559 \uae30\uc220 \uac1c\ubc1c\uc744 \ud1b5\ud574 \ub9ce\uc740 \uc804\ubb38 PCR \ud504\ub77c\uc774\uba38 \uc124\uacc4 \uc18c\ud504\ud2b8\uc6e8\uc5b4 \ubc0f \uc628\ub77c\uc778 \ub3c4\uad6c\uac00 \ub4f1\uc7a5\ud558\uc5ec \uc5f0\uad6c\uc6d0\uc774 PCR \ud504\ub77c\uc774\uba38\ub97c \ube60\ub974\uace0 \uc815\ud655\ud558\uac8c \uc124\uacc4 \ud560 \uc218 \uc788\uc2b5\ub2c8\ub2e4. \uc608\ub97c \ub4e4\uc5b4, Primer3, Oligocalc \ubc0f Primer-Blast\uc640 \uac19\uc740 \ub3c4\uad6c\ub294 \ud45c\uc801 \uc11c\uc5f4\uc5d0 \uae30\ucd08\ud558\uc5ec \ucd5c\uc801\uc758 \ud504\ub77c\uc774\uba38 \uae38\uc774, GC \ud568\ub7c9 \ubc0f \uc5b4\ub2d0\ub9c1 \uc628\ub3c4 \ud30c\ub77c\ubbf8\ud130\ub97c \uc790\ub3d9\uc73c\ub85c \uacc4\uc0b0\ud558\uace0 \uac00\ub2a5\ud55c \uc774\ub7c9 \uccb4 \ub610\ub294 \ud5e4\uc5b4\ud540 \uad6c\uc870\ub97c \uc608\uce21\ud558\uc5ec \ud504\ub77c\uc774\uba38 \uc124\uacc4\uc758 \ud6a8\uc728\uc131 \ubc0f \uc815\ud655\ub3c4\ub97c \ud06c\uac8c \ud5a5\uc0c1\uc2dc\ud0ac \uc218 \uc788\uc2b5\ub2c8\ub2e4.<\/p>\n\n\n\n 3. PCR \ud504\ub77c\uc774\uba38 \uc124\uacc4\uc5d0\uc11c \uc77c\ubc18\uc801\uc778 \ubb38\uc81c \ubc0f \uc194\ub8e8\uc158 1. \ube44\ud2b9\uc774\uc801 \uc99d\ud3ed : PCR \ubc18\uc751 \ub3d9\uc548 \ube44\ud2b9\uc774\uc801 \uc99d\ud3ed\uc774 \ubc1c\uc0dd\ud558\uba74 \ud504\ub77c\uc774\uba38\uc640 \uc8fc\ud615 DNA \uc0ac\uc774\uc758 \uacb0\ud569\uc774 \ucda9\ubd84\ud788 \ud2b9\uc774 \uc801\uc774 \uc9c0 \uc54a\uc744 \uc218 \uc788\uc2b5\ub2c8\ub2e4. \uc774 \uc2dc\uc810\uc5d0\uc11c \ud504\ub77c\uc774\uba38\uc758 \uc2dc\ud000\uc2a4\ub97c \uc870\uc815\ud558\uac70\ub098 PCR \uc870\uac74\uc744 \ucd5c\uc801\ud654\ud558\uac70\ub098\ubcf4\ub2e4 \ud2b9\uc815 \ud504\ub77c\uc774\uba38\ub97c \uc0ac\uc6a9\ud560 \uc218 \uc788\uc2b5\ub2c8\ub2e4. 2. \ud504\ub77c\uc774\uba38 \uc774\ub7c9 \uccb4 \ub610\ub294 \ud5e4\uc5b4\ud540 \uad6c\uc870 :\uc774 \ubb38\uc81c\ub294 \uc77c\ubc18\uc801\uc73c\ub85c \ud504\ub77c\uc774\uba38\uc758 \uc2dc\ud000\uc2a4\ub97c \uc870\uc815\ud558\uac70\ub098, \uc911\ubcf5 G \ub610\ub294 C \uc601\uc5ed\uc744 \ud53c\ud558\uac70\ub098, \uc801\uc808\ud55c \ud504\ub77c\uc774\uba38 \uc124\uacc4 \uc18c\ud504\ud2b8\uc6e8\uc5b4\ub85c \ucd5c\uc801\ud654\ud568\uc73c\ub85c\uc368 \ud574\uacb0\ud560 \uc218 \uc788\uc2b5\ub2c8\ub2e4. 3. \ube44\ud6a8\uc728\uc801 \uc778 \uc99d\ud3ed \ud6a8\uc728 : \ube44\ud6a8\uc728\uc801 \uc778 \uc99d\ud3ed\uc740 \ubd80\uc801\uc808\ud55c \ud504\ub77c\uc774\uba38 \uc124\uacc4 \ub610\ub294 \ubd80\uc801\uc808\ud55c PCR \uc870\uac74\uc73c\ub85c \uc778\ud55c \uac83\uc77c \uc218 \uc788\uc2b5\ub2c8\ub2e4. \ud504\ub77c\uc774\uba38\ub97c \uc7ac \uc124\uacc4\ud558\uac70\ub098 PCR \ubc18\uc751 \uc2dc\uc2a4\ud15c (\uc608 : \ud6a8\uc18c, \ud15c\ud50c\ub9bf \ub18d\ub3c4)\uc744 \ucd5c\uc801\ud654\ud568\uc73c\ub85c\uc368 \ud6a8\uc728\uc744 \ud5a5\uc0c1\uc2dc\ud0ac \uc218 \uc788\uc2b5\ub2c8\ub2e4.<\/p>\n\n\n\n PCR \ud504\ub77c\uc774\uba38\uc758 \uc124\uacc4\ub294 PCR \uc2e4\ud5d8\uc758 \uc131\uacf5\uc758 \uc8fc\uc694 \uc694\uc778 \uc911 \ud558\ub098\uc785\ub2c8\ub2e4. \uc798 \uc124\uacc4\ub41c \ud504\ub77c\uc774\uba38\ub294 \uc2e4\ud5d8\uc758 \ud2b9\uc774\uc131 \ubc0f \uc99d\ud3ed \ud6a8\uc728\uc744 \ud5a5\uc0c1\uc2dc\ud0ac\ubfd0\ub9cc \uc544\ub2c8\ub77c \uc2e4\ud5d8\uc758 \uc77c\ubc18\uc801\uc778 \uc624\ub958 \ubc0f \ubb38\uc81c\ub97c \uc904\uc77c \uc218 \uc788\uc2b5\ub2c8\ub2e4. PCR \ud504\ub77c\uc774\uba38\uc758 \uc124\uacc4 \uc6d0\ub9ac\ub97c \ub9c8\uc2a4\ud130\ud558\uace0 \uc801\uc808\ud55c \ub3c4\uad6c\uc640 \ubc29\ubc95\uc73c\ub85c \ucd5c\uc801\ud654\ud558\ub294 \uac83\uc740 \ubaa8\ub4e0 \ubd84\uc790 \uc0dd\ubb3c\ud559 \uc5f0\uad6c\uc6d0\uc5d0\uac8c \ud544\uc694\ud55c \uae30\uc220\uc785\ub2c8\ub2e4.<\/p>\n\n\n\n \uad00\ub828 \uc77d\uae30 \uad8c\uc7a5 \uc0ac\ud56d<\/p>\n\n\n\n \ud53c\ud3ab \ubc31\uc11c : 4 \uc778\uc758 \uc6d0\uc2ec \ubd84\ub9ac \ud30c\uc774\ud504 \uc2a4\ud0e0\ub4dc <\/a><\/p>\n\n\n\n \ubc31\uc11c : Opentrons High Precision Pipepter <\/a><\/p>\n\n\n\n OT-2 \ud3ec\uc2a4\ud130\uc5d0\uc11c THP-1 \uc138\ud3ec\uc5d0\uc11c \uacfc\uc0b0\ud654\uc218\uc18c \uc218\uc900\uc758 \uc790\ub3d9 \uacb0\uc815 <\/a><\/p>\n\n\n\n \ub2e8\ubc31\uc9c8 \uc815\uc81c \uc2dc\uc2a4\ud15c\uc758 \uc6d0\ub9ac \ubc0f \uc751\uc6a9 <\/a><\/p>\n\n\n\n<\/figure>\n\n\n\n