{"id":8509,"date":"2025-01-16T17:36:11","date_gmt":"2025-01-16T09:36:11","guid":{"rendered":"https:\/\/opentrons.com.cn\/news\/\/"},"modified":"2025-01-16T17:36:47","modified_gmt":"2025-01-16T09:36:47","slug":"dna-tiwaikuozengjishu","status":"publish","type":"post","link":"https:\/\/opentrons.com.cn\/ko\/news\/dna-tiwaikuozengjishu\/","title":{"rendered":"\uc2dc\ud5d8 \uad00\ub0b4 DNA \uc99d\ud3ed \uae30\uc220"},"content":{"rendered":"\n
\uc2dc\ud5d8 \uad00\ub0b4 DNA \uc99d\ud3ed \uae30\uc220\uc740 \uc774\ub984\uc5d0\uc11c \uc54c \uc218 \uc788\ub4ef\uc774 \ud2b9\uc815 \ubc29\ubc95 \ubc0f \ub3c4\uad6c\ub97c \ud1b5\ud574 \uc2e4\ud5d8\uc2e4 \ud658\uacbd\uc5d0\uc11c \ud45c\uc801 DNA \ubd84\uc790\uc758 \uc815\ub7c9\uc801 \uc99d\ud3ed\uc744 \uc9c0\uce6d\ud55c\ub2e4. \uc774 \uae30\uc220\uc740 \ubd84\uc790 \uc0dd\ubb3c\ud559 \uc5f0\uad6c, \uc720\uc804\uc790 \uacf5\ud559, \uc9c8\ubcd1 \uc9c4\ub2e8, \ubc95\uc758\ud559 \ubc0f \uae30\ud0c0 \ubd84\uc57c\uc5d0\uc11c \ub110\ub9ac \uc0ac\uc6a9\ub418\uc5c8\uc73c\uba70 \ud604\ub300 \uc0dd\ubb3c\ud559\uc801 \uc5f0\uad6c \ubc0f \uc2e4\uc2b5\uc5d0\uc11c \ud544\uc218 \uae30\uc220 \uc911 \ud558\ub098\uac00\ub418\uc5c8\uc2b5\ub2c8\ub2e4.<\/p>\n\n\n\n \uc2dc\ud5d8 \uad00\ub0b4 DNA \uc99d\ud3ed <\/a><\/p>\n\n\n\n 1. \uae30\ubcf8 \uc6d0\ub9ac \uc2dc\ud5d8 \uad00\ub0b4 DNA \uc99d\ud3ed \uae30\uc220\uc758 \uae30\ubcf8 \uc6d0\ub9ac\ub294 DNA \ud3f4\ub9ac\uba38 \ub77c\uc81c\uc758 \ucd09\ub9e4 \ud558\uc5d0\uc11c, \ubd80\ubaa8 \uac00\ub2e5 DNA\uac00 \uc5f0\uc7a5\uc758 \ucd9c\ubc1c\uc810\uc73c\ub85c\uc11c \uc8fc\ud615 \ubc0f \ud2b9\uc815 \ud504\ub77c\uc774\uba38\ub85c \uc0ac\uc6a9\ub418\uba70, \ubcc0\uc131, \uc5b4\ub2d0\ub9c1, \uc5f0\uc7a5 \ubc0f \uae30\ud0c0 \ub2e8\uacc4\ub97c \ud1b5\ud574 \ubd80\ubaa8 \uac00\ub2e5 \ud15c\ud50c\ub9bf DNA\uc5d0 \ub300\ud55c \uc5b4\ub9b0\uc774 \uc2a4\ud2b8\ub79c\ub4dc DNA \ubcf4\uccb4\uac00 \uc2dc\ud5d8\uad00 \ub0b4\uc5d0\uc11c \uc7ac\ud604\ub41c\ub2e4\ub294 \uac83\uc774\ub2e4. \uc774 \uacfc\uc815\uc5d0\uc11c, DNTP (DEXYNUCLEOTIDE), MG\u00b2A, \uc2e0\uc7a5 \uacc4\uc218 \ubc0f \uc99d\ud3ed \ud5a5\uc0c1 \uacc4\uc218\uac00 \uc2dc\uc2a4\ud15c\uc5d0 \ucd94\uac00\ub41c\ub2e4. 1. \ubcc0\uc131 : \uace0\uc628\uc744 \uc0ac\uc6a9\ud558\uc5ec DNA \uc774\uc911 \uac00\ub2e5\uc744 \ubd84\ub9ac\ud558\uc2ed\uc2dc\uc624. \uc774\uc911 \uac00\ub2e5 DNA \uc0ac\uc774\uc758 \uc218\uc18c \uacb0\ud569\uc740 \ub2e8\uc77c \uac00\ub2e5 DNA\ub97c \ud615\uc131\ud558\uae30 \uc704\ud574 \uace0\uc628 (\uc608 : 93 ~ 98 \u2103)\uc5d0\uc11c \uc911\ub2e8\ub41c\ub2e4. 2. \uc5b4\ub2d0\ub9c1 : DNA \uc774\uc911 \uac00\ub2e5 \ubd84\ub9ac \ud6c4, \ud504\ub77c\uc774\uba38\uac00 \ub2e8\uc77c \uac00\ub2e5 DNA\uc5d0 \uacb0\ud569 \ud560 \uc218 \uc788\ub3c4\ub85d \uc628\ub3c4\uac00 \uac10\uc18c\ub429\ub2c8\ub2e4. \ud504\ub77c\uc774\uba38\ub294 \ud45c\uc801 DNA \ub2e8\ud3b8\uc758 \uc591\ucabd \ub9d0\ub2e8\uc5d0 \uc0c1\ubcf4\ud55c \uc62c\ub9ac\uace0 \ub274\ud074\ub808\uc624\ud2f0\ub4dc \uc11c\uc5f4\uc774\uba70, \uc774\ub294 \ud45c\uc801 DNA\ub97c \uad6c\uccb4\uc801\uc73c\ub85c \uc778\uc2dd\ud558\uace0 \uacb0\ud569 \ud560 \uc218\uc788\ub2e4. 3. \uc5f0\uc7a5 : DNA \ud3f4\ub9ac\uba38 \ub77c\uc81c\uc758 \uc791\uc6a9 \ud558\uc5d0\uc11c, DNTP\ub97c \uc6d0\ub8cc\ub85c \uc0ac\uc6a9\ud558\uc5ec, \ud504\ub77c\uc774\uba38\uc758 3 '\ub9d0\ub2e8\uc73c\ub85c\ubd80\ud130 \uc0c8\ub85c\uc6b4 DNA \uac00\ub2e5\uc774 \ud569\uc131\ub41c\ub2e4. \uc774 \uacfc\uc815\uc5d0\uc11c, DNA \ud3f4\ub9ac\uba38 \ub77c\uc81c\ub294 \ud15c\ud50c\ub9bf \uccb4\uc778\uc744 \ub530\ub77c \uc774\ub3d9\ud558\uace0 \uc644\uc804\ud55c \uc0c1\ubcf4 \uccb4\uc778\uc774 \ud569\uc131 \ub420 \ub54c\uae4c\uc9c0 DNTP\ub97c \uc9c0\uc18d\uc801\uc73c\ub85c \ucd94\uac00\ud569\ub2c8\ub2e4. \ud55c\uc8fc\uae30\ub97c \uc644\ub8cc \ud55c \ud6c4, DNA \ub2e8\ud3b8\uc758 \uc218\ub294 \ub450 \ubc30\uac00\ub429\ub2c8\ub2e4. \ub2e4\uc911\uc8fc\uae30 (\ubcf4\ud1b5 25 ~ 35 \ubc30)\ub97c \ud1b5\ud574 DNA \ub2e8\ud3b8\uc758 \uc218\ub294 \uae30\ud558 \uae09\uc218\uc801\uc73c\ub85c \uc99d\uac00\ud569\ub2c8\ub2e4.<\/p>\n\n\n\n 2. \uc8fc\uc694 \uae30\uc220 - PCR \uae30\uc220 \uc2dc\ud5d8 \uad00\ub0b4 DNA \uc99d\ud3ed \uae30\uc220\uc758 \uac00\uc7a5 \ub300\ud45c\uc801\uc778 \uac83\uc740 PCR (Polymerase Chain Recalip) \uae30\uc220\uc785\ub2c8\ub2e4. PCR \uae30\uc220\uc740 1985 \ub144 \ubbf8\uad6d \uacfc\ud559\uc790 Mullis\uc5d0 \uc758\ud574 \ubc1c\uba85\ub418\uc5c8\uc73c\uba70 Science \uc800\ub110 \uc5d0\uc11c\uc774 \uae30\uc220\uc5d0 \uad00\ud55c \ud68d\uae30\uc801\uc778 \ub17c\ubb38\uc744 \ubc1c\ud45c\ud588\uc2b5\ub2c8\ub2e4. \uc774 \ubc1c\uba85\uc740 1993 \ub144\uc5d0 \ub178\ubca8 \ud654\ud559 \uc0c1\uc744 \uc218\uc0c1\ud588\uc744\ubfd0\ub9cc \uc544\ub2c8\ub77c DNA \uacf5\ud559 \uc5f0\uad6c \ubc29\ubc95\uc744 \uc644\uc804\ud788 \ubc14\uafb8\uace0 \uc0dd\uba85 \uacfc\ud559 \ubd84\uc57c\uc758 \uac1c\ubc1c\uc744 \ucd09\uc9c4\ud588\uc2b5\ub2c8\ub2e4. PCR \uae30\uc220\uc740 \uace0\ud6a8\uc728, \ud2b9\uc774\uc131 \ubc0f \uac10\ub3c4\uc758 \ud2b9\uc131\uc744 \uac00\uc9c0\uba70 \uc9e7\uc740 \uc2dc\uac04\uc5d0 \ub2e4\uc218\uc758 \ud45c\uc801 DNA \ub2e8\ud3b8\uc744 \uc99d\ud3ed\uc2dc\ud0ac \uc218 \uc788\uc2b5\ub2c8\ub2e4. PCR \uae30\uc220\uc744 \ud1b5\ud574 \uc5f0\uad6c\uc790\ub4e4\uc740 \uc720\uc804\uc790 \ud074\ub85c\ub2dd, \uc9c8\ubcd1 \uc9c4\ub2e8, \ubc95\uc758\ud559 \ubc0f \uae30\ud0c0 \ubd84\uc57c\uc5d0 \uad11\ubc94\uc704\ud55c \uc751\uc6a9\uc744 \uac16\ub294 \ud2b9\uc815 DNA \uc11c\uc5f4\uc744 \uc27d\uac8c \uc99d\ud3ed\uc2dc\ud0ac \uc218 \uc788\uc2b5\ub2c8\ub2e4.<\/p>\n\n\n\n 3. PCR \uae30\uc220\uc758 \ubd84\ub958 \ub2e4\ub978 \uc751\uc6a9 \ubc0f \uc694\uad6c\uc5d0 \ub530\ub77c PCR \uae30\uc220\uc740 \uc5ec\ub7ec \uc720\ud615\uc73c\ub85c \ub098\ub20c \uc218 \uc788\uc2b5\ub2c8\ub2e4. 1. \uc77c\ubc18 PCR : \uc99d\ud3ed\uc740 \uc77c\ubc18\uc801\uc778 DNA \ud3f4\ub9ac\uba38 \ub77c\uc81c\uc5d0 \uc758\ud574 \uc0ac\uc6a9 \ub41c \ub2e4\uc74c \uc0dd\uc131\ubb3c\uc740 \uc544\uac00 \ub85c\uc2a4 \uac94 \uc804\uae30 \uc601\ub3d9\uc5d0 \uc758\ud574 \uac10\uc9c0\ub429\ub2c8\ub2e4. \uc77c\ubc18 PCR\uc740 \uc815 \uc131\uc801 \ubd84\uc11d \ub9cc \uc218\ud589 \ud560 \uc218 \uc788\uc2b5\ub2c8\ub2e4. \uc989, \ud45c\uc801 DNA\uac00 \uc874\uc7ac\ud558\ub294\uc9c0 \uc5ec\ubd80\ub97c \uacb0\uc815\ud558\uae30 \uc704\ud574\uc11c\ub294 \uc815\ub7c9\uc801 \uac80\ucd9c\uc744 \uc218\ud589 \ud560 \uc218 \uc5c6\uc2b5\ub2c8\ub2e4. 2. \ud615\uad11 \uc815\ub7c9\uc801 PCR (\uc2e4\uc2dc\uac04 PCR \ub610\ub294 QPCR) : \ud615\uad11 \uc2e0\ud638\ub97c \ucd95\uc801\ud558\uc5ec \uc99d\ud3ed \ub41c \uc0dd\uc131\ubb3c\uc758 \ucd95\uc801\uc744 \ubaa8\ub2c8\ud130\ub9c1\ud558\uae30 \uc704\ud574 \ubc18\uc751 \uacfc\uc815\uc744 \ub098\ud0c0\ub0b4\ub294 \ud615\uad11 \ud504\ub85c\ube0c\ub97c \ucd94\uac00\ud569\ub2c8\ub2e4. \ud615\uad11 \uc815\ub7c9\uc801 PCR\uc740 \uc815\ub7c9\uc801 \uac80\ucd9c, \uc989 \ud45c\uc801 DNA\uc758 \ub18d\ub3c4 \ub610\ub294 \uce74\ud53c \uc218\ub97c \uacb0\uc815\ud560 \uc218\uc788\ub2e4. \ud615\uad11 \ubb3c\uc9c8\uc740 Taqman \ud615\uad11 \ud504\ub85c\ube0c, \ubd84\uc790 \ube44\ucf58 \ubc0f \ud615\uad11 \uc5fc\ub8cc\ub85c \ub098\ub20c \uc218 \uc788\uc2b5\ub2c8\ub2e4. 3. \ub514\uc9c0\ud138 PCR (Digital PCR) : \uc5d4\ub4dc \ud3ec\uc778\ud2b8 \uac10\uc9c0\ub97c \ud1b5\ud574 \ub300\uc0c1 \uc2dc\ud000\uc2a4\uc758 \uce74\ud53c \uc218\ub97c \uacc4\uc0b0\ud558\uace0 \ub0b4\ubd80 \ub9e4\uac1c \ubcc0\uc218 \ubc0f \ud45c\uc900 \uace1\uc120\uc744 \uc0ac\uc6a9\ud558\uc9c0 \uc54a\uace0 \uc815\ud655\ud558\uace0 \uc808\ub300\uc801\uc73c\ub85c \uc815\ub7c9\uc801 \uc778 \uac10\uc9c0\ub97c \uc218\ud589 \ud560 \uc218 \uc788\uc2b5\ub2c8\ub2e4. \ub514\uc9c0\ud138 PCR\uc740 \ub192\uc740 \uac10\ub3c4 \ubc0f \ub192\uc740 \uc815\ud655\ub3c4\uc758 \ud2b9\uc131\uc744 \uac00\uc9c0\uba70 PCR \ubc18\uc751 \uc5b5\uc81c\uc81c\uc5d0 \uc758\ud574 \uc27d\uac8c \ubc29\ud574\ub418\uc9c0 \uc54a\uc73c\uba70 \ud45c\uc900\uc758 \ud544\uc694\uc5c6\uc774 \uc9c4\uc815\ud55c \uc808\ub300 \uc815\ub7c9\ud654\ub97c \ub2ec\uc131 \ud560 \uc218 \uc788\uc2b5\ub2c8\ub2e4. \ubc18\uc751 \ub2e8\uc704\uc758 \ub2e4\ub978 \ud615\ud0dc\uc5d0 \ub530\ub77c, \ub514\uc9c0\ud138 PCR\uc740 \ubbf8\uc138 \uc720\uccb4, \uce69 \ubc0f \ub9c8\uc774\ud06c\ub85c \ub85c\ud50c\ub81b \uc2dc\uc2a4\ud15c\uc758 \uc138 \uac00\uc9c0 \ubc94\uc8fc\ub85c \ub098\ub20c \uc218 \uc788\uc2b5\ub2c8\ub2e4.<\/p>\n\n\n\n 4. \uc2dc\ud5d8 \uad00\ub0b4 DNA \uc99d\ud3ed \uae30\uc220\uc758 \uc801\uc6a9 \ubc0f \uc804\ub9dd \uc0dd\uba85 \uacfc\ud559 \ubd84\uc57c\uc5d0\uc11c \uad11\ubc94\uc704\ud55c \uc801\uc6a9 \uc804\ub9dd\uc774\uc788\ub2e4. 1. \uc720\uc804\uc790 \ud074\ub85c\ub2dd : PCR \uae30\uc220\uc740 \ud2b9\uc815 \uc720\uc804\uc790 \ub2e8\ud3b8\uc744 \uc99d\ud3ed\uc2dc\ud0a8 \ub2e4\uc74c \ubca1\ud130\uc5d0 \uc0bd\uc785\ud558\uc5ec \uc7ac\uc870\ud569 DNA \ubd84\uc790\ub97c \uad6c\uc131\ud558\uc5ec \uc720\uc804\uc790 \ud074\ub85c\ub2dd\uc744 \ub2ec\uc131 \ud560 \uc218\uc788\ub2e4. 2. \uc9c8\ubcd1 \uc9c4\ub2e8 : PCR \uae30\uc220\uc740 \ubcd1\uc6d0\uccb4 \uc720\uc804\uc790, \ub3cc\uc5f0\ubcc0\uc774 \uc720\uc804\uc790 \ub4f1\uacfc \uac19\uc740 \uc9c8\ubcd1\uacfc \uad00\ub828\ub41c \ud2b9\uc815 DNA \ub2e8\ud3b8\uc744 \uc99d\ud3ed\uc2dc\ud0a4\ub294 \ub370 \uc0ac\uc6a9\ub420 \uc218 \uc788\uc73c\uba70, \uc774\ub85c \uc778\ud574 \uc9c8\ubcd1\uc758 \uc9c4\ub2e8 \ubc0f \uce58\ub8cc\uc5d0 \ub300\ud55c \uac15\ub825\ud55c \uae30\ucd08\ub97c \uc81c\uacf5\ud569\ub2c8\ub2e4. 3. \ubc95\uc758\ud559 : \ubc95\uc758\ud559 \ubd84\uc57c\uc5d0\uc11c PCR \uae30\uc220\uc740 \ud608\uc561 \uc5bc\ub8e9, \ubaa8\ubc1c, \uc870\uc9c1 \uc870\uac01 \ub4f1\uacfc \uac19\uc740 \ubbf8\ub7c9 DNA \uc0d8\ud50c\uc744 \ucd94\ucd9c\ud558\uace0 \ubd84\uc11d\ud558\ub294 \ub370 \uc0ac\uc6a9\ub418\uba70, \uc0ac\ub840 \uc870\uc0ac\uc5d0 \ub300\ud55c \uc8fc\uc694 \uc99d\uac70\ub97c \uc81c\uacf5\ud569\ub2c8\ub2e4. 4. \uc0dd\ubb3c \ub2e4\uc591\uc131 \uc5f0\uad6c : PCR \uae30\uc220\uc740 \ub2e4\ub978 \uc885\uc758 \ud2b9\uc815 DNA \ub2e8\ud3b8\uc744 \uc99d\ud3ed\uc2dc\ud0a8 \ub2e4\uc74c \uc720\uc804 \uc801 \ub2e4\uc591\uc131\uacfc \uc9c4\ud654 \uad00\uacc4\ub97c \uc5f0\uad6c \ud560 \uc218 \uc788\uc2b5\ub2c8\ub2e4.<\/p>\n\n\n\n \uc2dc\ud5d8 \uad00\ub0b4 DNA \uc99d\ud3ed \uae30\uc220, \ud2b9\ud788 PCR \uae30\uc220\uc740 \ud604\ub300 \uc0dd\uba85 \uacfc\ud559 \uc5f0\uad6c \ubc0f \uc758\ub8cc \uc9c4\ub2e8\uc5d0\uc11c \ub300\uccb4 \ud560 \uc218\uc5c6\ub294 \uc5ed\ud560\uc744\ud569\ub2c8\ub2e4. \uae30\uc220\uc758 \uc9c0\uc18d\uc801\uc778 \ubc1c\uc804\uc73c\ub85c, \uc2dc\ud5d8 \uad00\ub0b4 DNA \uc99d\ud3ed \uae30\uc220\uc740 \ub354 \ub9ce\uc740 \ubd84\uc57c\uc5d0\uc11c \ubcc0\ud654\ub97c \uac00\uc838\uc62c \uac83\uc774\ub2e4. \uc720\uc804\uc790 \ubcf5\uc81c, \uc9c8\ubcd1 \uc9c4\ub2e8 \ub610\ub294 \ubc95\uc758\ud559 \ubd84\uc57c \uc5d0\uc11c\uc774 \uae30\uc220\uc740 \uacfc\ud559\uc790\ub4e4\uc5d0\uac8c \uac15\ub825\ud55c \ub3c4\uad6c\ub97c \uc81c\uacf5\ud588\uc73c\uba70 \uc778\uac04 \uac74\uac15, \uc0dd\uba85 \uacfc\ud559 \ubc0f \uc0ac\ud68c \uac1c\ubc1c\uc744 \uc0c8\ub85c\uc6b4 \ucc28\uc6d0\uc73c\ub85c \ud64d\ubcf4\ud588\uc2b5\ub2c8\ub2e4.<\/p>\n\n\n\n \uad00\ub828 \uc77d\uae30 \uad8c\uc7a5 \uc0ac\ud56d<\/p>\n\n\n\n \uac00\uc5f4 \uc9c4\ub3d9 \ubaa8\ub4c8 \uc18c\uac1c <\/a><\/p>\n\n\n\n \uc0ac\uc6a9\uc790 \uc124\uba85\uc11c : Opentrons \ub2e8\uc77c \ucc44\ub110 \ud53c\ud3ab <\/a><\/p>\n\n\n\n \uc5b4\ub311\ud130 \uc790\uc11d \uc9c0\uc6d0 \uae30\uc0ac <\/a><\/p>\n\n\n\n Opentrons\uc758 \uc720\uc5f0\ud55c \uc4f0\ub808\uae30\ud1b5\uc758 \uccad\uc18c \ud575\uc0b0 \ucd94\ucd9c \uc6cc\ud06c \uc2a4\ud14c\uc774\uc158 <\/a><\/p>\n\n\n\n<\/figure>\n\n\n\n