{"id":3713,"date":"2024-07-30T18:15:19","date_gmt":"2024-07-30T10:15:19","guid":{"rendered":"https:\/\/opentrons.com.cn\/?post_type=knowledge2&p=3713"},"modified":"2024-09-02T18:21:21","modified_gmt":"2024-09-02T10:21:21","slug":"pcr-kuozengyi","status":"publish","type":"post","link":"https:\/\/opentrons.com.cn\/ko\/news\/pcr-kuozengyi\/","title":{"rendered":"PCR\u6269\u589e\u4eea"},"content":{"rendered":"\n

PCR\u6269\u589e\u4eea\uff0c\u53c8\u79f0\u4e3aPCR\u57fa\u56e0\u6269\u589e\u4eea\u3001PCR\u6838\u9178\u6269\u589e\u4eea\u6216\u805a\u5408\u9176\u94fe\u53cd\u5e94\u6838\u9178\u6269\u589e\u4eea\uff0c\u662f\u4e00\u79cd\u5229\u7528PCR\uff08Polymerase Chain Reaction\uff0c\u805a\u5408\u9176\u94fe\u53cd\u5e94\uff09\u6280\u672f\u5bf9\u7279\u5b9aDNA\u8fdb\u884c\u6269\u589e\u7684\u4eea\u5668\u8bbe\u5907\u3002\u8fd9\u79cd\u6280\u672f\u5728\u533b\u5b66\u3001\u751f\u7269\u5b66\u5b9e\u9a8c\u5ba4\u4e2d\u5f97\u5230\u4e86\u5e7f\u6cdb\u5e94\u7528\uff0c\u5305\u62ec\u7528\u4e8e\u5224\u65ad\u68c0\u4f53\u4e2d\u662f\u5426\u4f1a\u8868\u73b0\u67d0\u9057\u4f20\u75be\u75c5\u7684\u56fe\u8c31\u3001\u4f20\u67d3\u75c5\u7684\u8bca\u65ad\u3001\u57fa\u56e0\u590d\u5236\u4ee5\u53ca\u4eb2\u5b50\u9274\u5b9a\u7b49\u3002<\/p>\n\n\n\n

\"PCR\u6269\u589e\u4eea\"<\/figure>\n\n\n\n

\u4e00\u3001PCR\u6269\u589e\u4eea\u7684\u5de5\u4f5c\u539f\u7406<\/p>\n\n\n\n

PCR\u6269\u589e\u4eea\u7684\u5de5\u4f5c\u539f\u7406\u57fa\u4e8ePCR\u6280\u672f\uff0c\u8be5\u6280\u672f\u901a\u8fc7\u5faa\u73af\u53d8\u6027-\u9000\u706b-\u5ef6\u4f38\u4e09\u4e2a\u57fa\u672c\u6b65\u9aa4\uff0c\u5728\u4f53\u5916\u5c06\u7279\u5b9a\u7684DNA\u7247\u6bb5\u5728\u77ed\u65f6\u95f4\u5185\u8fdb\u884c\u6570\u767e\u4e07\u500d\u7684\u6269\u589e\u3002\u5177\u4f53\u6765\u8bf4\uff1a<\/p>\n\n\n\n

1\u3001\u53d8\u6027\uff1a\u5728\u9ad8\u6e29\uff08\u901a\u5e38\u4e3a95\u2103\uff09\u4e0b\uff0cDNA\u53cc\u94fe\u6253\u5f00\u6210\u5355\u94fe\u3002<\/p>\n\n\n\n

2\u3001\u9000\u706b\uff1a\u6e29\u5ea6\u964d\u81f3\u9002\u5b9c\u8303\u56f4\uff08\u598255\u2103\uff09\uff0c\u5f15\u7269\u4e0e\u5355\u94feDNA\u7684\u4e92\u8865\u5e8f\u5217\u7ed3\u5408\u3002<\/p>\n\n\n\n

3\u3001\u5ef6\u4f38\uff1a\u5728DNA\u805a\u5408\u9176\uff08\u5982Taq\u9176\uff09\u7684\u4f5c\u7528\u4e0b\uff0c\u4ee5dNTPs\uff08dATP\u3001dTTP\u3001dCTP\u3001dGTP\uff09\u4e3a\u53cd\u5e94\u539f\u6599\uff0c\u6309\u7167\u78b1\u57fa\u914d\u5bf9\u4e0e\u534a\u4fdd\u7559\u590d\u5236\u539f\u7406\uff0c\u5408\u6210\u4e00\u6761\u65b0\u7684\u4e0e\u6a21\u677fDNA\u94fe\u4e92\u8865\u7684\u534a\u4fdd\u7559\u590d\u5236\u94fe\u3002<\/p>\n\n\n\n

\u8fd9\u4e09\u4e2a\u6b65\u9aa4\u53cd\u590d\u8fdb\u884c\uff0c\u6bcf\u4e00\u8f6e\u5faa\u73af\u90fd\u4f7fDNA\u7279\u5f02\u533a\u62f7\u8d1d\u6570\u6269\u589e\u4e00\u500d\uff0c\u7ecf\u8fc7\u591a\u8f6e\u5faa\u73af\u540e\uff0c\u7406\u8bba\u4e0a\u53ef\u4f7f\u57fa\u56e0\u6269\u589e\u6570\u767e\u4e07\u500d\u4ee5\u4e0a\u3002<\/p>\n\n\n\n

\u4e8c\u3001PCR\u6269\u589e\u4eea\u7684\u5206\u7c7b<\/p>\n\n\n\n

\u6839\u636e\u529f\u80fd\u548c\u7528\u9014\u7684\u4e0d\u540c\uff0cPCR\u6269\u589e\u4eea\u53ef\u4ee5\u5206\u4e3a\u591a\u79cd\u7c7b\u578b\uff0c\u5982\u666e\u901aPCR\u4eea\u3001\u68af\u5ea6PCR\u4eea\u3001\u539f\u4f4dPCR\u4eea\u548c\u5b9e\u65f6\u8367\u5149\u5b9a\u91cfPCR\u4eea\u7b49\u3002<\/p>\n\n\n\n

1\u3001\u666e\u901aPCR\u4eea\uff1a\u4e00\u6b21\u53ea\u80fd\u8fd0\u884c\u4e00\u4e2a\u7279\u5b9a\u9000\u706b\u6e29\u5ea6\u7684PCR\u6269\u589e\uff0c\u4e3b\u8981\u7528\u4e8e\u7b80\u5355\u7684\u3001\u5bf9\u76ee\u7684\u57fa\u56e0\u9000\u706b\u6e29\u5ea6\u7684\u6269\u589e\u3002<\/p>\n\n\n\n

2\u3001\u68af\u5ea6PCR\u4eea\uff1a\u53ef\u4ee5\u8bbe\u7f6e\u4e00\u7cfb\u5217\u4e0d\u540c\u7684\u9000\u706b\u6e29\u5ea6\u6761\u4ef6\uff0c\u7528\u4e8e\u7814\u7a76\u672a\u77e5DNA\u9000\u706b\u6e29\u5ea6\u7684\u6269\u589e\uff0c\u53ef\u4ee5\u8282\u7ea6\u6210\u672c\u548c\u65f6\u95f4\u3002<\/p>\n\n\n\n

3\u3001\u539f\u4f4dPCR\u4eea\uff1a\u7528\u4e8e\u4ece\u7ec6\u80de\u5185\u9776DNA\u7684\u5b9a\u4f4d\u5206\u6790\uff0c\u4fdd\u6301\u7ec6\u80de\u6216\u7ec4\u7ec7\u7684\u5b8c\u6574\u6027\uff0c\u5728\u7ec6\u80de\u7684\u9776DNA\u6240\u5728\u4f4d\u7f6e\u4e0a\u8fdb\u884c\u57fa\u56e0\u6269\u589e\u3002<\/p>\n\n\n\n

4\u3001\u5b9e\u65f6\u8367\u5149\u5b9a\u91cfPCR\u4eea\uff1a\u901a\u8fc7\u5b9e\u65f6\u76d1\u6d4b\u8367\u5149\u4fe1\u53f7\u7684\u53d8\u5316\u6765\u5b9a\u91cf\u68c0\u6d4bPCR\u4ea7\u7269\uff0c\u5177\u6709\u66f4\u9ad8\u7684\u7075\u654f\u5ea6\u548c\u51c6\u786e\u6027\u3002<\/p>\n\n\n\n

\u4e09\u3001PCR\u6269\u589e\u4eea\u7684\u7279\u70b9<\/p>\n\n\n\n

1\u3001\u7cbe\u786e\u63a7\u6e29\uff1aPCR\u6269\u589e\u4eea\u5185\u90e8\u91c7\u7528\u52a0\u70ed\u5757\u6216\u52a0\u70ed\u82af\u6765\u4fdd\u6301\u6e29\u5ea6\u6052\u5b9a\uff0c\u5e76\u4f7f\u7528\u6e29\u5ea6\u4f20\u611f\u5668\u8fdb\u884c\u7cbe\u786e\u6d4b\u6e29\uff0c\u786e\u4fddPCR\u53cd\u5e94\u5728\u6700\u4f73\u6e29\u5ea6\u6761\u4ef6\u4e0b\u8fdb\u884c\u3002<\/p>\n\n\n\n

2\u3001\u81ea\u52a8\u5316\u548c\u7a0b\u5e8f\u5316\uff1a\u53ef\u4ee5\u8bbe\u7f6e\u4e0d\u540c\u7684\u53cd\u5e94\u7a0b\u5e8f\uff0c\u5b9e\u73b0\u591a\u79cdPCR\u53cd\u5e94\u7684\u540c\u65f6\u8fdb\u884c\uff0c\u63d0\u9ad8\u5b9e\u9a8c\u6548\u7387\u3002<\/p>\n\n\n\n

3\u3001\u591a\u901a\u9053\u68c0\u6d4b\uff1a\u4e00\u4e9b\u9ad8\u7ea7\u7684PCR\u6269\u589e\u4eea\u8fd8\u914d\u5907\u4e86\u591a\u901a\u9053\u68c0\u6d4b\u529f\u80fd\uff0c\u53ef\u4ee5\u5b9e\u65f6\u76d1\u6d4b\u53cd\u5e94\u8fdb\u7a0b\u5e76\u83b7\u53d6\u5b9a\u91cf\u7ed3\u679c\uff0c\u4fbf\u4e8e\u6570\u636e\u5206\u6790\u3002<\/p>\n\n\n\n

\u56db\u3001PCR\u6269\u589e\u4eea\u7684\u5e94\u7528\u9886\u57df<\/p>\n\n\n\n

PCR\u6269\u589e\u4eea\u88ab\u5e7f\u6cdb\u5e94\u7528\u4e8e\u533b\u5b66\u3001\u751f\u7269\u5b66\u3001\u98df\u54c1\u5de5\u4e1a\u3001\u53f8\u6cd5\u79d1\u5b66\u3001\u751f\u7269\u6280\u672f\u3001\u73af\u5883\u79d1\u5b66\u3001\u5fae\u751f\u7269\u5b66\u3001\u4e34\u5e8a\u8bca\u65ad\u3001\u6d41\u884c\u75c5\u5b66\u3001\u9057\u4f20\u5b66\u3001\u57fa\u56e0\u82af\u7247\u3001\u57fa\u56e0\u68c0\u6d4b\u3001\u57fa\u56e0\u514b\u9686\u3001\u57fa\u56e0\u8868\u8fbe\u7b49\u9886\u57df\u3002\u5b83\u5e2e\u52a9\u79d1\u7814\u4eba\u5458\u5feb\u901f\u3001\u51c6\u786e\u5730\u68c0\u6d4b\u548c\u5206\u6790\u7279\u5b9a\u7684DNA\u7247\u6bb5\uff0c\u5bf9\u4e8e\u75be\u75c5\u7684\u8bca\u65ad\u3001\u57fa\u56e0\u7814\u7a76\u4ee5\u53ca\u836f\u7269\u5f00\u53d1\u7b49\u65b9\u9762\u5177\u6709\u91cd\u8981\u610f\u4e49\u3002<\/p>\n\n\n\n

\u4e94\u3001\u6ce8\u610f\u4e8b\u9879<\/p>\n\n\n\n

\u5728\u4f7f\u7528PCR\u6269\u589e\u4eea\u65f6\uff0c\u9700\u8981\u6ce8\u610f\u4ee5\u4e0b\u51e0\u70b9\uff1a<\/p>\n\n\n\n

1\u3001\u8bbe\u5907\u6d88\u6bd2\u548c\u6e05\u6d01\uff1a\u907f\u514d\u6837\u54c1\u6c61\u67d3\uff0c\u786e\u4fdd\u5b9e\u9a8c\u7ed3\u679c\u7684\u51c6\u786e\u6027\u3002<\/p>\n\n\n\n

2\u3001\u6309\u7167\u64cd\u4f5c\u6b65\u9aa4\u8fdb\u884c\uff1a\u4e0d\u8981\u968f\u610f\u66f4\u6539\u53c2\u6570\u6216\u64cd\u4f5c\u6d41\u7a0b\uff0c\u4ee5\u514d\u5f71\u54cd\u5b9e\u9a8c\u7ed3\u679c\u3002<\/p>\n\n\n\n

3\u3001\u6ce8\u610f\u7535\u6e90\u548c\u7535\u6781\u5b89\u5168\uff1a\u907f\u514d\u7535\u51fb\u4e8b\u6545\u7684\u53d1\u751f\u3002<\/p>\n\n\n\n

4\u3001\u64cd\u4f5c\u5b8c\u6210\u540e\u8fdb\u884c\u6e05\u6d01\u548c\u7ef4\u62a4\uff1a\u4ee5\u4fdd\u8bc1\u8bbe\u5907\u7684\u6b63\u5e38\u4f7f\u7528\u548c\u5bff\u547d\u3002<\/p>\n\n\n\n

PCR\u6269\u589e\u4eea\u662f\u4e00\u79cd\u91cd\u8981\u7684\u5206\u5b50\u751f\u7269\u5b66\u5b9e\u9a8c\u8bbe\u5907\uff0c\u5176\u5de5\u4f5c\u539f\u7406\u57fa\u4e8ePCR\u6280\u672f\uff0c\u5177\u6709\u7cbe\u786e\u63a7\u6e29\u3001\u81ea\u52a8\u5316\u548c\u7a0b\u5e8f\u5316\u3001\u591a\u901a\u9053\u68c0\u6d4b\u7b49\u7279\u70b9\uff0c\u88ab\u5e7f\u6cdb\u5e94\u7528\u4e8e\u591a\u4e2a\u9886\u57df\u3002\u5728\u4f7f\u7528\u8fc7\u7a0b\u4e2d\u9700\u8981\u6ce8\u610f\u76f8\u5173\u4e8b\u9879\u4ee5\u786e\u4fdd\u5b9e\u9a8c\u7ed3\u679c\u7684\u51c6\u786e\u6027\u548c\u8bbe\u5907\u7684\u5b89\u5168\u6027\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

PCR\u6269\u589e\u4eea\uff0c\u53c8\u79f0\u4e3aPCR\u57fa\u56e0\u6269\u589e\u4eea\u3001PCR\u6838\u9178\u6269\u589e\u4eea\u6216\u805a\u5408\u9176\u94fe\u53cd\u5e94\u6838\u9178\u6269\u589e\u4eea\uff0c\u662f\u4e00\u79cd\u5229\u7528PCR\uff08Polyme […]<\/p>\n","protected":false},"author":5,"featured_media":3715,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"inline_featured_image":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[],"tags":[],"class_list":["post-3713","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry"],"yoast_head":"\nPCR\u6269\u589e\u4eea - Opentrons\u4e2d\u56fd\u5b98\u7f51<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/opentrons.com.cn\/ko\/news\/pcr-kuozengyi\/\" \/>\n<meta property=\"og:locale\" content=\"zh_CN\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"PCR\u6269\u589e\u4eea - 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