{"id":2812,"date":"2024-05-30T21:43:40","date_gmt":"2024-05-30T13:43:40","guid":{"rendered":"https:\/\/opentrons.com.cn\/?post_type=knowledge2&p=2812"},"modified":"2024-09-03T17:12:02","modified_gmt":"2024-09-03T09:12:02","slug":"5-reasons-to-automate","status":"publish","type":"knowledge2","link":"https:\/\/opentrons.com.cn\/ja\/knowledge2\/5-reasons-to-automate\/","title":{"rendered":"\u81ea\u52a8\u5316 NGS \u5de5\u4f5c\u6d41\u7a0b\u7684 5 \u4e2a\u7406\u7531"},"content":{"rendered":"\n

\u4e0e\u7b2c\u4e00\u4ee3\u65b9\u6cd5\u76f8\u6bd4\uff0c\u65b0\u4e00\u4ee3\u6d4b\u5e8f (NGS) \u5177\u6709\u66f4\u5feb\u7684\u901f\u5ea6\u3001\u66f4\u4f4e\u7684\u6210\u672c\u548c\u66f4\u9ad8\u7684\u901a\u91cf\uff0c\u5df2\u5c06\u751f\u7269\u5b66\u7814\u7a76\u63a8\u5165\u4e86\u4e00\u4e2a\u65b0\u65f6\u4ee3\u3002 \u5168\u57fa\u56e0\u7ec4\u6d4b\u5e8f\u3001\u8868\u89c2\u57fa\u56e0\u7ec4\u5b66\u3001\u5b8f\u57fa\u56e0\u7ec4\u5b66\u548c\u5fae\u751f\u7269\u7ec4\u5b66\u53ea\u662f\u5f53\u4eca\u4f17\u591a NGS \u5e94\u7528\u4e2d\u7684\u4e00\u5c0f\u90e8\u5206\uff0c\u5b83\u4eec\u4fc3\u8fdb\u4e86\u6d41\u884c\u75c5\u5b66\u3001\u764c\u75c7\u8bca\u65ad\u548c\u836f\u7269\u9886\u57df\u7684\u8fdb\u6b65\u3002<\/p>\n\n\n\n

\"\u81ea\u52a8\u5316<\/figure>\n\n\n\n

\u4e0e\u7b2c\u4e00\u4ee3\u65b9\u6cd5\u76f8\u6bd4\uff0c\u4e0b\u4e00\u4ee3\u6d4b\u5e8f (NGS) \u5177\u6709\u66f4\u5feb\u7684\u901f\u5ea6\u3001\u66f4\u4f4e\u7684\u6210\u672c\u548c\u66f4\u9ad8\u7684\u901a\u91cf\uff0c\u5df2\u5c06\u751f\u7269\u5b66\u7814\u7a76\u63a8\u5165\u4e86\u4e00\u4e2a\u65b0\u65f6\u4ee3\u3002 \u5168\u57fa\u56e0\u7ec4\u6d4b\u5e8f\u3001\u8868\u89c2\u57fa\u56e0\u7ec4\u5b66\u3001\u5b8f\u57fa\u56e0\u7ec4\u5b66\u548c\u5fae\u751f\u7269\u7ec4\u5b66\u53ea\u662f\u5f53\u4eca\u4f17\u591a NGS \u5e94\u7528\u4e2d\u7684\u4e00\u5c0f\u90e8\u5206\uff0c\u5b83\u4eec\u4fc3\u8fdb\u4e86\u6d41\u884c\u75c5\u5b66\u3001\u764c\u75c7\u8bca\u65ad\u548c\u836f\u7269\u53d1\u73b0\u7684\u8fdb\u6b65\uff0c\u5e76\u6781\u5927\u5730\u63d0\u9ad8\u4e86\u6211\u4eec\u5bf9\u751f\u7269\u4f53\u5982\u4f55\u5229\u7528\u9057\u4f20\u4fe1\u606f\u5728\u73af\u5883\u4e2d\u751f\u5b58\u548c\u7e41\u6b96\u7684\u7406\u89e3\u3002 \u4e0d\u65ad\u53d8\u5316\u7684\u73af\u5883\u3002<\/p>\n\n\n\n

\u5e02\u573a\u4e0a\u6709\u5404\u79cd\u5404\u6837\u7684\u6d4b\u5e8f\u4eea\u5668\u548c\u670d\u52a1\u63d0\u4f9b\u5546\uff0cNGS \u6b63\u8fc5\u901f\u88ab\u5404\u79cd\u89c4\u6a21\u548c\u4e0d\u540c\u9884\u7b97\u7684\u5b9e\u9a8c\u5ba4\u6240\u63a5\u53d7\u3002 \u867d\u7136\u6d4b\u5e8f\u548c\u6570\u636e\u5206\u6790\u901a\u5e38\u662f\u81ea\u52a8\u5316\u7684\uff0c\u4f46\u4e0a\u6e38 NGS \u6b65\u9aa4\uff0c\u5373\u6837\u54c1\u5904\u7406\u548c\u6587\u5e93\u5236\u5907\u901a\u5e38\u662f\u624b\u52a8\u8fdb\u884c\u7684\u3002 \u5e78\u8fd0\u7684\u662f\uff0c\u901a\u8fc7\u4e00\u4e9b\u52aa\u529b\u548c\u4f18\u5316\uff0c\u8fd9\u4e9b\u6b65\u9aa4\u53ef\u4ee5\u5728\u79fb\u6db2\u673a\u5668\u4eba\u4e0a\u8f7b\u677e\u5b9e\u73b0\u81ea\u52a8\u5316\uff0c\u4ece\u800c\u4e3a\u7528\u6237\u8282\u7701\u4e86\u8bb8\u591a\u6f5c\u5728\u7684\u9ebb\u70e6\u3002 \u5728\u8fd9\u91cc\uff0c\u6211\u4eec\u6765\u770b\u770b\u81ea\u52a8\u5316 NGS \u5de5\u4f5c\u6d41\u7a0b\u7684 5 \u5927\u7406\u7531\u3002<\/p>\n\n\n\n

    \n
  1. \u53ef\u6269\u5c55\u6027<\/strong><\/li>\n<\/ol>\n\n\n\n

    \u5982\u679c\u60a8\u66fe\u7ecf\u5c1d\u8bd5\u8fc7\u624b\u52a8\u5904\u7406\u5927\u91cf NGS \u7ed3\u5408\u6837\u672c\uff0c\u90a3\u4e48\u6269\u5927\u89c4\u6a21\u7684\u6f5c\u529b\u5e94\u8be5\u4f1a\u8ba9\u60a8\u5bf9\u81ea\u52a8\u5316\u611f\u5230\u5174\u594b\u3002 \u673a\u5668\u4eba\u7684\u5177\u4f53\u529f\u80fd\u53d6\u51b3\u4e8e\u578b\u53f7\uff0c\u4f46\u5728\u5927\u591a\u6570\u60c5\u51b5\u4e0b\uff0c\u60a8\u5c06\u80fd\u591f\u540c\u65f6\u5904\u7406\u591a\u8fbe 96 \u4e2a\u6837\u672c\uff0c\u4ece\u800c\u663e\u7740\u51cf\u5c11\u624b\u52a8\u5904\u7406\u76f8\u540c\u6570\u91cf\u7684\u6837\u672c\u6240\u9700\u7684\u65f6\u95f4\u3002<\/p>\n\n\n\n

    \u81ea\u52a8\u5316\u8fd8\u4e3a\u60a8\u7701\u53bb\u4e86\u6279\u91cf\u5904\u7406\u6837\u54c1\u7684\u53ef\u6015\u9ebb\u70e6\uff0c\u7531\u4e8e\u79bb\u5fc3\u673a\u5c3a\u5bf8\u3001\u6837\u54c1\u964d\u89e3\u98ce\u9669\uff08\u4f8b\u5982\uff0c\u4f7f\u7528 RNA \u65f6\uff09\u4ee5\u53ca\u6700\u5927\u6570\u91cf\u7684\u9650\u5236\uff0c\u8fd9\u5728\u624b\u52a8\u8bbe\u7f6e\u4e2d\u901a\u5e38\u662f\u5fc5\u8981\u7684\u3002 \u4eba\u4eec\u5728\u4efb\u4f55\u65f6\u5019\u90fd\u611f\u89c9\u53ef\u4ee5\u8f7b\u677e\u5904\u7406\u7684\u6837\u672c\u3002 \u6bd4\u907f\u514d\u9ebb\u70e6\u66f4\u91cd\u8981\u7684\u662f\uff0c\u66f4\u5c11\u7684\u6279\u6b21\u610f\u5473\u7740\u66f4\u5c11\u6216\u6ca1\u6709\u6279\u6b21\u95f4\u7684\u5dee\u5f02\u3002<\/p>\n\n\n\n

    2\u3001\u521b\u65b0<\/strong><\/p>\n\n\n\n

    \u901a\u8fc7\u81ea\u52a8\u5316\u6269\u5927 NGS \u5b9e\u9a8c\u89c4\u6a21\u5c06\u4e0d\u53ef\u907f\u514d\u5730\u8ba9\u60a8\u80fd\u591f\u89e3\u51b3\u624b\u52a8\u8bbe\u7f6e\u51e0\u4e4e\u65e0\u6cd5\u89e3\u51b3\u7684\u65b0\u7814\u7a76\u95ee\u9898\u3002 \u4ee5\u4e0b\u662f\u4e00\u7cfb\u5217 NGS \u5e94\u7528\u7684\u51e0\u4e2a\u793a\u4f8b\uff1a<\/p>\n\n\n\n

    \u836f\u7269\u53d1\u73b0\uff1a\u7b5b\u9009\u5927\u91cf\u65b0\u578b\u5316\u5408\u7269\uff0c\u4ee5\u5bfb\u627e\u75be\u75c5\u6750\u6599\uff08\u4f8b\u5982\u7ec6\u80de\u7cfb\uff09\u4e2d\u6240\u9700\u7684\u8f6c\u5f55\u6216\u8868\u89c2\u9057\u4f20\u53d8\u5316\uff0c\u800c\u4e0d\u662f\u8bd5\u56fe\u6311\u9009\u53ef\u80fd\u5177\u6709\u6cbb\u7597\u76f8\u5173\u4f5c\u7528\u7684\u5316\u5408\u7269\u3002<\/p>\n\n\n\n

    \u5fae\u751f\u7269\u7ec4\u5b66\uff1a\u5206\u6790\u8db3\u591f\u5927\u7684\u6837\u672c\u96c6\uff0c\u4ee5\u83b7\u5f97\u60a8\u9009\u62e9\u7684\u7269\u79cd\u4e2d\u201c\u6b63\u5e38\u201d\u5fae\u751f\u7269\u533a\u7cfb\u7684\u56fe\u7247\u3002 \u5982\u679c\u60a8\u4e00\u6b21\u53ea\u80fd\u5904\u7406 12 \u6216 24 \u4e2a\u6837\u672c\uff0c\u8fd9\u5c06\u662f\u4e00\u9879\u4e0d\u53ef\u80fd\u5b8c\u6210\u7684\u4efb\u52a1\u3002<\/p>\n\n\n\n

    \u690d\u7269\u80b2\u79cd\uff1a\u5927\u89c4\u6a21\u9274\u5b9a\u519c\u827a\u91cd\u8981\u57fa\u56e0\u548c\u9057\u4f20\u6807\u8bb0\uff0c\u4ee5\u5e2e\u52a9\u57fa\u56e0\u7ec4\u9009\u62e9\u548c\u57fa\u56e0\u5de5\u7a0b\u5f00\u53d1\u5177\u6709\u9ad8\u5ea6\u7406\u60f3\u6027\u72b6\u7684\u65b0\u54c1\u79cd\u3002<\/p>\n\n\n\n

    \u75be\u75c5\u7814\u7a76\u548c\u8bca\u65ad\uff1a\u7b5b\u9009\u5927\u91cf\u4e34\u5e8a\u6837\u672c\u4ee5\u53d1\u73b0\u65b0\u7684\u75be\u75c5\u76f8\u5173\u57fa\u56e0\u6216\u751f\u7269\u6807\u5fd7\u7269\u3002<\/p>\n\n\n\n

    \u73af\u5883\u76d1\u6d4b\uff1a\u901a\u8fc7\u5bf9\u5176\u65e0\u810a\u690e\u52a8\u7269\u5c45\u6c11\u8fdb\u884c\u5b9a\u6027\u548c\u5b9a\u91cf\u5e8f\u5217\u5206\u6790\u6765\u8bc4\u4f30\u751f\u6001\u7cfb\u7edf\u7684\u5065\u5eb7\u72b6\u51b5\u3002<\/p>\n\n\n\n

    \u9664\u4e86\u589e\u52a0\u60a8\u81ea\u5df1\u7684\u7814\u7a76\u521b\u65b0\u4e4b\u5916\uff0c\u66f4\u9ad8\u7684\u901a\u91cf\u8fd8\u5e94\u8be5\u4fc3\u8fdb\u4e0e\u5176\u4ed6\u5b9e\u9a8c\u5ba4\u52a0\u5f3a\u5408\u4f5c\uff0c\u4e3a\u65b0\u9879\u76ee\u548c\u53d1\u73b0\u6253\u5f00\u5927\u95e8\uff0c\u5426\u5219\u8fd9\u4e9b\u9879\u76ee\u548c\u53d1\u73b0\u5c06\u975e\u5e38\u5177\u6709\u6311\u6218\u6027\uff08\u5982\u679c\u53ef\u80fd\u7684\u8bdd\uff09\u3002<\/p>\n\n\n\n

      \n
    1. \u4e00\u81f4\u7684\u6570\u636e\u8d28\u91cf<\/li>\n<\/ol>\n\n\n\n

      \u5728\u6574\u4e2aNGS\u5de5\u4f5c\u6d41\u7a0b\u4e2d\uff0c\u6837\u672c\u5904\u7406\u3001\u6587\u5e93\u6784\u5efa\u4ee5\u53caPCR\u6269\u589e\u548c\u6587\u5e93\u6807\u51c6\u5316\u7b49\u4e0b\u6e38\u5904\u7406\u6b65\u9aa4\u7279\u522b\u5bb9\u6613\u51fa\u73b0\u4eba\u4e3a\u9519\u8bef\u3002 \u8be5\u534f\u8bae\u5f88\u5197\u957f\uff0c\u6709\u8bb8\u591a\u79fb\u6db2\u6b65\u9aa4\u548c\u4e0d\u65ad\u53d8\u5316\u7684\u8bd5\u5242\u4f53\u79ef\uff0c\u5e76\u4e14\u901a\u5e38\u9700\u8981\u6cbf\u9014\u5c06\u6837\u54c1\u8f6c\u79fb\u5230\u65b0\u7ba1\u4e2d\u3002 \u4e0d\u6b63\u786e\u7684\u6807\u7b7e\u3001\u6c61\u67d3\u3001\u79fb\u6db2\u9519\u8bef\u6216\u4e0e\u65b9\u6848\u7684\u6700\u8f7b\u5fae\u504f\u5dee\u90fd\u4f1a\u4e25\u91cd\u5f71\u54cd\u7ed3\u679c\u6570\u636e\u3002<\/p>\n\n\n\n

      \u5373\u4f7f\u4eba\u4e3a\u9519\u8bef\u5b8c\u5168\u4e0d\u53ef\u907f\u514d\uff0c\u6279\u6b21\u4e4b\u95f4\u7684\u5dee\u5f02\u4ee5\u53ca\u5de5\u4f5c\u4eba\u5458\u4e4b\u95f4\u6837\u54c1\u5904\u7406\u7684\u5dee\u5f02\u4e5f\u662f\u4e0d\u53ef\u907f\u514d\u7684\u3002 \u5982\u679c\u53ef\u80fd\u7684\u8bdd\uff0c\u76d1\u63a7 NGS \u5de5\u4f5c\u6d41\u7a0b\u4e2d\u7684\u8fd9\u4e9b\u9519\u8bef\u548c\u53d8\u5316\u4e5f\u662f\u4e0d\u5207\u5b9e\u9645\u7684\u3002 \u81ea\u52a8\u5316\u6837\u54c1\u548c\u6587\u5e93\u5236\u5907\u7f13\u89e3\u4e86\u8fd9\u4e00\u95ee\u9898\uff0c\u63d0\u4f9b\u66f4\u597d\u7684\u8fc7\u7a0b\u63a7\u5236\u3001\u51c6\u786e\u9ad8\u6548\u7684\u5de5\u4f5c\u6d41\u7a0b\u4ee5\u53ca\u72ec\u7acb\u4e8e\u4eba\u5458\u53d8\u52a8\u7684\u66f4\u597d\u7684\u8fd0\u884c\u91cd\u590d\u6027\uff0c\u4ece\u800c\u65e0\u8bba\u60a8\u6709 1 \u4e2a\u8fd8\u662f 1,000 \u4e2a\u6837\u54c1\uff0c\u90fd\u53ef\u4ee5\u63d0\u4f9b\u53ef\u9760\u6027\u548c\u4e00\u81f4\u7684\u6570\u636e\u8d28\u91cf\u3002<\/p>\n\n\n\n

        \n
      1. \u589e\u52a0\u79bb\u5f00\u65f6\u95f4<\/strong><\/li>\n<\/ol>\n\n\n\n

        \u8fd9\u5e76\u4e0d\u5b8c\u5168\u662f\u9ed1\u767d\u5206\u660e\u7684\uff0c\u56e0\u4e3a\u60a8\u9700\u8981\u5728\u542f\u52a8\u9636\u6bb5\u6295\u5165\u4e00\u4e9b\u65f6\u95f4\u548c\u7cbe\u529b\uff0c\u4ee5\u786e\u4fdd\u60a8\u7684\u79fb\u6db2\u673a\u5668\u4eba\u80fd\u591f\u6267\u884c\u4e25\u683c\u7684\u534f\u8bae\u3002\u5982\u679c\u5e78\u8fd0\u7684\u8bdd\uff0c\u60a8\u4e5f\u8bb8\u80fd\u591f\u627e\u5230\u4e00\u4e2a \u4ece\u5176\u4f9b\u5e94\u5546\u6216\u60a8\u9009\u62e9\u7684\u6838\u9178\u63d0\u53d6\u548c\u6587\u5e93\u5236\u5907\u8bd5\u5242\u76d2\u7684\u4f9b\u5e94\u5546\u5904\u83b7\u53d6\u9002\u5408\u60a8\u673a\u5668\u4eba\u7684\u811a\u672c\uff0c\u5176\u4e2d\u8bb8\u591a\u4f9b\u5e94\u5546\u4e0e\u81ea\u52a8\u5316\u4f9b\u5e94\u5546\u5408\u4f5c\u5f00\u53d1\u81ea\u52a8\u5316\u5c31\u7eea\u534f\u8bae\u3002<\/p>\n\n\n\n

        \u65e0\u8bba\u5982\u4f55\uff0c\u4e00\u65e6\u60a8\u7684\u81ea\u52a8\u5316\u8bbe\u7f6e\u542f\u52a8\u5e76\u8fd0\u884c\uff0c\u60a8\u5c31\u53ef\u4ee5\u4eab\u53d7\u5c06\u5b9d\u8d35\u7684\u5b9e\u9a8c\u5ba4\u65f6\u95f4\u82b1\u5728\u89c4\u5212\u65b0\u7684\u5173\u952e\u5b9e\u9a8c\u4e0a\uff0c\u800c\u4e0d\u662f\u91cd\u590d\u7684\u6837\u54c1\u548c\u6587\u5e93\u51c6\u5907\u3002 \u81ea\u52a8\u5316\u8fd8\u53ef\u4ee5\u51cf\u5c11\u5bf9\u5458\u5de5\u8fdb\u884c\u591a\u79cd\u4e0d\u540c\u65b9\u6848\u57f9\u8bad\u6240\u9700\u7684\u5de5\u4f5c\u91cf\uff0c\u4ece\u800c\u91ca\u653e\u66f4\u591a\u8d44\u6e90\uff0c\u5e76\u907f\u514d\u5458\u5de5\u56e0\u8fc7\u5ea6\u79fb\u6db2\u800c\u9020\u6210\u7684\u91cd\u590d\u6027\u52b3\u635f\u3002<\/p>\n\n\n\n

          \n
        1. \u964d\u4f4e\u6210\u672c<\/strong><\/li>\n<\/ol>\n\n\n\n

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          \u4f60\u80fd\u81ea\u52a8\u5316\u5b8c\u6210\u5de5\u4f5c\u5417\uff1f<\/p>\n\n\n\n

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