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OT-2 ng S

OT-2 ng S Workstation

Proven workstation application Contact us NGS library preparation for up to 24 samples

The NGS library preparation workstation includes: OT-2 pipetting robot, pipettes, tips, labware, thermal cycler module, magnetic module, temperature control module and custom protocols.

Broadly compatible with reagents from a variety of manufacturers, the OT-2 NGS Workstation automates library preparation and pre-sequencing workflows, including fragmentation and labeling protocols.

  • Library construction

  • Magnetic bead cleaning

  • Normalize and mix

  • Library sample preparation

  • Tags and conversions

Packing list

Hardware, consumables, and custom protocols developed for automated NGS library preparation.

  • Robots and pipettes
    • OT-2 pipetting robot
    • Single-channel high-precision pipette (P20)
    • 8-channel high-precision pipette (P300)
    • Developed custom protocols
  • Module
    • Magnetic module
    • Temperature control module
    • Thermal cycling module
  • Labware
    • 2 boxes of Opentrons 20 uL filtered tip racks (2 x 9,600 tips)
    • 2 boxes of Opentrons 200 uL filtered tip racks (2 x 9,600 tips)
    • NEST 12-hole reservoir (50 pcs.)
    • Rugged 0.2 mL 96-Well PCR Plate (Pack of 25)
    • NEST 2.0 mL Microcentrifuge Tubes (500)

DNA preparation method demonstrated on the OT-2 NGS workstation

Figure 1. ng SW IT hot-2. the dotted line box indicate then GS steps that can be automated on the OT-2

Resource

Application Notes

protocol

Brochures and white papers

FAQ

Frequently asked questions about Opentrons Flex and OT-2 products，Please click here.

  • What kind of labware is the OT-2 compatible with?

    Out of the box, the OT-2 can support any automation-compatible, ANSI/SLAS-compliant orifice plate, as well as a wide variety of EP tubes and screw-cap centrifuge tubes, including centrifuge tubes with volumes up to 50 mL. You can view all supported labware in our labware library.

  • Do I need to know programming knowledge to use OT-2?

    No programming knowledge is required to use the Opentrons platform. You can create protocols using Opentrons' simple and easy-to-understand protocol design tools.In addition, our application engineering team can also Create a custom agreement for you.

  • What does the OT-2 protocol look like?

    You can find our community's OT-2 protocol collection in our protocol library.You can search to find a protocol that matches your lab's workflow! If you don't find a protocol that matches your lab's workflow, we offer several different ways to create your protocol. In addition, you can contact us to have our team of engineers develop a custom protocol for you.

  • Can the OT-2 be integrated with third-party lab equipment?

    Due to the open source advantage of OT-2, many users have successfully integrated OT-2 with Third-party laboratory equipment.

  • What certifications does the OT-2 have?

    OT-2 has the following certifications: CE, FCC, NRTL, ISO 9001, CB. Please note that this OT-2 is not IVD or GMP certified/validated.

  • Can OT-2 be used in a biological safety cabinet?

    Labs within the Opentrons community have installed OT-2 automated pipetting platforms in biosafety cabinets, including Labconco’s Logic Vue Class II closure device and Baker’s AeroPROTECT 360 closure device. We recommend that you evaluate the size of the OT-2 by comparing it with the size of the biological safety cabinet you choose.

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