In daily operations of scientific research and laboratories, pipettes are key equipment for precise manipulation of trace amounts of liquids, and the stability and reliability of their performance are crucial. However, the problem of pipette leakage is like an invisible obstacle in the experimental process. It not only interferes with the accuracy of the experiment, but may also cause unnecessary waste of experimental materials and even mislead the experimental results.
1. Common damaged parts 1. Damage to the sealing gasket (such as O-ring): (1) The sealing gasket is a key component in the pipette and is responsible for maintaining the sealing inside the pipette. (2) After long-term use, the sealing gasket may be aged, worn or deformed, resulting in loose sealing and leakage. (3) Solution: Regularly check and replace aging or damaged seals to ensure good sealing performance. 2. Damage to the piston and sleeve: (1) The matching accuracy between the piston and the sleeve is crucial to the sealing performance of the pipette. (2) If the piston or sleeve is worn, deformed or damaged, the sealing performance may be reduced, resulting in liquid leakage. (3) Solution: According to the requirements of the instruction manual, regularly maintain and inspect the piston, sleeve and other parts of the pipette. If necessary, replace damaged parts in time. 3. The suction nozzle is damaged or inappropriate: (1) The suction nozzle is the part of the pipette that is in direct contact with the liquid. Its quality and adaptability have a great impact on the pipetting effect. (2) If the pipette tip is damaged, deformed, or does not match the pipette, liquid leakage may occur. (3) Solution: Choose a pipette tip suitable for the pipette and make sure it is intact. Before use, check whether the pipette nozzle fits tightly with the pipette to avoid leakage. 4. Damage to other parts: (1) If other parts in the pipette, such as springs, push rods, etc., are damaged or fail, they may also indirectly cause leakage. (2) For example, spring relaxation may cause the piston to not fit tightly, causing fluid leakage. (3) Solution: Conduct comprehensive inspection and maintenance of the pipette regularly, and find and replace damaged parts in time.
2. Operating specifications 1. Install and use the suction head correctly and avoid being too tight or too loose. 2. When using a pipette, you should follow operating specifications, such as moving the control button slowly, staying under the liquid surface for a sufficient time, etc. 3. Avoid using pipettes to absorb highly volatile and corrosive liquids to avoid damaging pipette parts. 4. Calibrate the pipette regularly to ensure its accuracy and precision.
The problem of pipette leakage cannot be ignored, and its root cause is mostly damage to key components. From the aging of the seal, the wear of the piston, to the deformation or damage of the pipette nozzle, every detail is related to the precise performance of the pipette and the reliability of the experimental results. Therefore, as laboratory workers, we must not only master the correct operation methods of pipettes, but also conduct detailed inspection and maintenance of pipettes regularly, discover and replace damaged parts in time, and ensure the best work of pipettes. state.
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