During the experiment, it is difficult to completely avoid instrument damage caused by incorrect operation. Especially when the pipette comes into contact with liquid due to careless operation, it is particularly important to take measures quickly and effectively to protect the pipette from damage and ensure that subsequent experiments can proceed smoothly. This article will discuss in depth the emergency treatment plan after the pipette fills the liquid, aiming to provide practical guidance for scientific researchers.
1. Preliminary treatment 1. Stop operation immediately: Once you find that the pipette has entered liquid, you should stop using it immediately to avoid further contamination or damage to the pipette. 2. Replace the tip: If the liquid only adheres to the outer surface of the tip but does not enter the inside of the pipette, you can replace it with a dry tip. Before replacing, make sure the outside of the tip is dry to avoid contaminating the sample with the liquid attached.
2. In-depth processing 1. Empty the residual liquid: If the liquid has entered the pipe of the pipette, the residual liquid needs to be drained first. 2. Cleaning and disinfection: Soak the pipette in 70% alcohol or bleach solution for a period of time (the soaking time and solution concentration are determined according to the material and instructions of the pipette), and then rinse thoroughly with deionized water or pure water. . This can effectively remove residual liquid and contaminants in the pipeline.
3. Inspection and maintenance 1. Check the pipette: After cleaning and disinfection, carefully check whether all parts of the pipette are intact, especially sealing parts such as the piston and O-ring. If it is damaged or aged, it should be replaced in time. 2. Drying treatment: Place the pipette in a dry environment to dry naturally to ensure that there is no residual moisture inside. 3. Calibration: If the performance of the pipette is affected after cleaning and disinfection, it may need to be calibrated to ensure its accuracy. 4. If you find that the liquid in the electric pipette is serious, you may need to repair or replace parts. During this process, it is recommended that you choose a professional pipette repair organization or contact the manufacturer to ensure the quality and safety of the repair.
4. Preventive measures 1. Use the pipette correctly: When operating the pipette, try to keep the tip lifted vertically to avoid tilting it and causing liquid to enter the pipe. At the same time, your hands should be light to avoid excessive force and excessive negative pressure, which may cause damage to the suction head. 2. Choose the appropriate pipette: Choose the appropriate pipette volume according to the viscosity and concentration of the liquid, and do not exceed the pipette's range. 3. Regular maintenance: Clean, disinfect and calibrate the pipette regularly to ensure it is in good working condition.
Once it is discovered that the pipette has accidentally entered liquid, the operation must be stopped immediately and corresponding rescue steps must be performed immediately. With proper handling and precautions, we can minimize the damage that liquids can cause to our pipettes, while ensuring that their accuracy during experiments is not compromised.
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