Pipette Specifications

In precision laboratory operations, pipettes are an indispensable tool, and the selection of their specifications is directly related to the accuracy and reliability of experimental results. From micro-scale nanometers to large-scale milliliters, pipettes come in a variety of specifications designed to meet the specific needs of different experimental scenarios. The correct selection of pipette specifications can not only improve experimental efficiency, but also ensure the accuracy of every pipetting operation, providing solid data support for scientific research.

1. Commonly used specifications Commonly used pipette specifications (maximum range) in student experiments and daily scientific research generally include the following: 2.5μl 10μl 20μl 50μl 100μl 200μl 1000μl Pipettes of these specifications can cover the needs of liquid transfer from micro to medium amounts.

2. Measuring range The available measuring range of a pipette is usually 10% to 100% of its maximum range. Therefore, the available measuring ranges of each of the above pipettes are: 0.25~2.5μl1~10μl2~20μl5~50μl10~100μl20~200μl100~1000μl

3. Optimum usage range The best usage range of a pipette is usually considered to be 1/3 of its maximum range to its maximum range. Therefore, the best use ranges of the above pipettes are: 0.7~2.5μl3~10μl6~20μl16~50μl33~100μl66~200μl330~1000μl Using pipettes within this range can usually achieve higher accuracy and Accuracy.

4. Other specifications In addition to the common specifications mentioned above, pipettes also have larger or smaller specifications to choose from to meet specific experimental needs. For example, some laboratories may need to use micropipettes with a capacity of 0.1 μl or less, or high-volume pipettes with a capacity of 5000 μl or more.

5. Pipette selection principles 1. Range matching: The range of the selected pipette should be close to the volume of liquid that needs to be transferred in the experiment. Doing so ensures pipetting accuracy, as pipettes generally have their best precision and accuracy near their nominal capacity. Precision may decrease if the volume pipetted is much smaller than the pipette's minimum volume or close to but far from its maximum volume. 2. Accuracy and repeatability: The accuracy and repeatability indicators of a pipette are important criteria for measuring its performance. Precision refers to the deviation between the actual pipette volume and the set volume, while repeatability refers to the consistency when pipetting the same volume multiple times under the same conditions. High-precision and repeatable pipettes provide more reliable and reproducible experimental results. 3. Brand and quality: Different brands and models of pipettes may have differences in design and manufacturing, which affects their performance. Generally speaking, well-known brand and high-quality pipettes are more stringent in terms of material selection, manufacturing process, and quality control, and therefore have better performance and longer service life. 4. Ease of use and comfort: The operation of the pipette should be simple and easy and consistent with ergonomic principles to reduce user fatigue and misoperation. For example, the pipette button should be easy to press with clear feedback, and the handle should be comfortable to hold and not prone to slipping. 5. Special needs: In some experiments, it may be necessary to use pipettes with special functions, such as multi-channel pipettes, electric pipettes, or pipettes with specific tip adaptability. The consideration of these special needs is also a factor that cannot be ignored when selecting a pipette.

6. Precautions for use 1. When installing the suction head, insert it vertically and rotate it slightly to ensure a tight connection. 2. When aspirating and discharging liquids, keep the pipette vertical and operate at a constant speed to avoid bubbles and liquid residue. 3. After use, the pipette should be adjusted to the maximum capacity and hung on the pipette stand to extend its service life. 4. It is prohibited to place the pipette horizontally or upside down to prevent liquid from flowing into the piston chamber and corroding the internal parts.

When summarizing the selection and application of pipette specifications, we emphasize the importance of precise matching and efficient operation. Whether you are conducting highly sensitive molecular biology experiments or processing large numbers of samples for biochemical analysis, choosing the appropriate pipette specification is a critical step to ensure the success of your experiment.