Pipettes are an indispensable tool in modern laboratories. Their precise and efficient liquid transfer functions provide great convenience for scientific research, medical treatment and various experimental fields. The excellent performance of this precision instrument is inseparable from the complex and delicate design of its internal components. Each component plays a vital role. They cooperate and cooperate with each other accurately to ensure that the pipette can complete various liquid transfer tasks accurately and quickly.
1. Main components of the pipette 1. Display window: used to display the currently set volume of the pipette to help the user accurately control the amount of liquid to be pipetted. 2. Volume adjustment part (knob): By rotating this part, the user can adjust the pipetting volume required by the pipette. 3. Piston: The piston is one of the core components of the pipette. Through the telescopic movement of the spring, the piston can move up and down inside the pipette, thereby achieving the suction and discharge of liquid. 4. O-ring: As a sealing component, the O-ring ensures that the pipette will not leak during the aspiration and dispensing processes. 5. Suction tube (sleeve): The pipe connecting the piston and the suction head, responsible for guiding the flow of liquid. 6. Suction head (liquid suction nozzle): The part in direct contact with the liquid is usually made of plastic and has excellent corrosion resistance and sealing properties. 7. Control button: used to control the aspiration and dispensing operations of the pipette, usually located on the top or side of the pipette. 8. Suction tip removal button: used to easily replace or discard suction tips.
2. Working principle of pipette 1. The working principle of pipette is based on the expansion and contraction force of spring and the up and down movement of piston. Specifically, when the piston moves upwards driven by a spring, some of the air inside the pipette is expelled, creating a negative pressure at the tip. This negative pressure causes liquid to be drawn into the tip. When the piston moves downward, it pushes the air and liquid in the suction head out together, thereby achieving liquid transfer. 2. During operation, the user can adjust the required pipetting volume by rotating the volume adjustment component. After setting the volume, start the aspiration or dispensing process by pressing the control button. In order to ensure the accuracy of pipetting, users usually need to hold the tip in the liquid for a while to stabilize the liquid level after aspirating the liquid, and when dispensing the liquid, keep the tip close to the wall of the container to avoid liquid splashing. 3. Pipettes are also divided into two pipetting modes: built-in piston type and external piston type to adapt to the pipetting needs of different liquids. The built-in piston pipette is suitable for pipetting regular liquids, while the external piston pipette is more suitable for pipetting highly viscous, highly volatile or precious liquid samples.
As a precision instrument in the laboratory, the pipette's efficient and accurate liquid transfer capabilities are inseparable from the precise design and coordinated work of each component. From the display window to the volume adjustment component, from the piston to the O-ring, to the suction tube, tip and control button, every component plays an indispensable role, jointly ensuring the stability and stability of the pipette. reliability.
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