What are the main factors that determine the quality of a pipette?

Pipette, also known as pipette gun, is an instrument used for quantitative transfer of liquids. It is widely used in biology, chemistry and other fields and clinical diagnostic laboratories, biotechnology laboratories, pharmaceutical and chemical laboratories, environmental laboratories, Food laboratories, etc. The pipette works using the principle of air displacement. The piston realizes liquid suction and discharge through the telescopic movement of the spring. After setting the measuring range, push the piston to expel part of the air, use the piston pulling force and atmospheric pressure to suck in the liquid, and then use the piston thrust to discharge the liquid. Therefore, the airtightness of the pipette is particularly important.

There are three main factors that affect the accuracy and accuracy of pipetting: 1> Tip: The quality of the tip (the workmanship and material of the tip can affect the liquid residue on the inner wall of the tip and the accuracy of pipetting. Currently, domestic Although the price of the pipette tips is low, the quality has not yet reached the standard, so it is recommended to use imported pipette tips); the matching degree between the pipette tip and the pipette (suction tips The matching degree between the tip and the pipette affects the air tightness between the tip and the pipette, so it is very important to choose the matching tip and pipette. It is recommended to use pipettes and pipette tips from the same manufacturer to ensure that. Its matching degree) 2> Pipette: The pipette performs pipetting based on the principle of air displacement. Its sealing and elastic deformation of the spring are the key factors affecting pipetting. Therefore, when you use a pipette in daily life, you must operate it correctly and maintain it regularly to ensure the accuracy of the pipette. 3>Operator: The key is actually the operator himself. Developing correct operating specifications can improve the accuracy of pipetting, greatly reduce pipetting errors, and ensure experimental results.