When carrying out precision operations in the laboratory, the pipette is an indispensable core tool for liquid transfer. The selection of its specification and volume is crucial to ensuring the accuracy and efficiency of the experiment. Therefore, specifications and measuring range have become two key factors that must be considered when selecting and using pipettes in the laboratory.
1. Specifications of pipette guns Specifications of pipette guns usually include descriptions of their structure, materials, functions, etc. Common pipette types include manual pipettes and electronic pipettes. 1. Manual pipette: It is a more traditional and basic pipette, mainly composed of a tip, a tube body, a core, a piston and an adjustment knob. It has a simple structure, is cheap, is easy to maintain, and is suitable for pipetting operations of small volumes and small amounts of liquid. However, since the operation requires manual control, pipettes are easily affected by the operator's technical level and the external environment, and are less precise in use. 2. Electric pipette: It is an electrically driven liquid distributor. The electric drive makes the piston between the suction head and the tip move up and down, thereby achieving accurate distribution of liquid. Compared with manual pipettes, electric pipettes are more convenient and precise to use, and can automatically complete a certain amount of liquid distribution tasks. Electric pipettes can also be divided into multi-channel automatic pipettes, single-channel electric pipettes, multi-channel manual control electric pipettes, etc. according to their capacity and functional characteristics. In addition, the pipette may also have different channel numbers, such as single channel, multi-channel (such as 12-channel pipette), etc., to meet the needs of different experiments.
2. The measuring range of the pipetting gun The measuring range of the pipetting gun refers to the volume range within which it can accurately transfer liquid. The choice of measuring range is crucial to the accuracy and efficiency of the experiment. 1. Measuring range: The measuring range of a pipette gun usually varies according to its design. Common measuring ranges include 0.5~5ml, 100~1000μl, 20~200μl, 10~100μl, 2~20μl, 0.5~10μl and 0.1~2.5μl, etc. The volume ranges vary from brand to brand, but are usually clearly marked on the pipette. 2. Best operating range: Although the pipette can transfer liquid within its marked range, in order to improve accuracy and reduce errors, it is usually recommended to operate within the best operating range. Generally speaking, the optimal range of a pipette is from its maximum range to about 1/3 of its maximum range. For example, for a 200 μL pipette, its optimal range may be 50 to 200 μL. 3. Range adjustment and setting: Before using the pipette, it needs to be adjusted to the appropriate range according to experimental needs. The measurement range is usually adjusted by rotating the adjustment knob on the transfer gun. When adjusting the range, be careful not to over-rotate or use too much force to avoid damaging the pipette or affecting its accuracy. At the same time, after setting the measuring range, calibration and verification are also required to ensure the accuracy of the pipette.
3. Selection Suggestions When choosing a pipette gun, in addition to considering its specifications and range, you also need to consider other factors, such as accuracy, repeatability, applicability, brand reputation and after-sales service. These factors will jointly affect the performance and use of the pipette. Therefore, when choosing a pipette, it is recommended to conduct sufficient comparison and evaluation to ensure that you choose a product that meets your experimental needs.
Pipette size and capacity are two key factors to consider when selecting and using a pipette. By understanding the characteristics and applicable scope of pipettes of different specifications and ranges, and considering other related factors, you can ensure that you choose a product that meets your experimental needs, thereby improving the accuracy and efficiency of your experiment.
Related reading recommendations
Pipette White Paper: 4-in-1 Centrifuge Tube Rack
Handling robot instrument service overview
Automated determination of hydrogen peroxide levels in THP-1 cells on OT-2 poster
Opentrons biological suction tip types
Workflow of 96-channel fully automatic liquid handling workstation
The experienced service team and strong production support team provide customers with worry-free order services.
