The principle and working steps of enzymatic hydrolysis using a fully automatic pipetting workstation

The fully automatic pipetting workstation plays an important role in biological experiments, especially in enzymatic hydrolysis experiments, and can provide efficient and precise operations. Enzymatic hydrolysis refers to the process of using enzymatic reactions to break down complex biological macromolecules into small molecules, and the fully automatic pipetting workstation improves the efficiency of this process through automation technology.

Fully automatic pipetting workstation

1. Principle The principle of enzymatic hydrolysis performed by the fully automatic pipetting workstation is mainly based on its precise liquid control system, automated operation of the robotic arm, and collaborative work with other laboratory equipment. 1. Liquid control system: The fully automatic pipetting workstation achieves precise control of liquids through high-precision pumps, valves, sensors and other components. In enzymatic hydrolysis experiments, this system can ensure that the enzyme solution is accurately drawn and transferred to the sample to be enzymatically hydrolyzed in a preset amount. 2. Robotic arm and automated operation: The robotic arm is an important part of the fully automatic pipetting workstation and is responsible for carrying the suction head and moving it on the experimental table. The suction head is used to directly contact the liquid to complete the suction and release operations. The robotic arm moves the tip above the enzyme solution container according to the preset path and speed, and then moves it above the sample to be enzymatically hydrolyzed for release. 3. Collaborative work: The fully automatic pipetting workstation can be used in conjunction with other laboratory equipment (such as constant temperature oscillator) to control the temperature, pH value and other conditions of the enzymatic reaction. These conditions have an important impact on the rate and efficiency of enzymatic hydrolysis reactions.

2. Work steps The work steps of the fully automatic pipetting workstation for enzymatic hydrolysis usually include the following stages: 1. Equipment preparation and initialization: Place the fully automatic pipetting workstation on a stable, vibration-free table and connect the power cord. Turn on the power switch and wait for the workstation to start up and complete initialization. According to the experimental needs, set the pipetting parameters, such as pipetting volume, pipetting speed, pipetting and discharging positions, etc. 2. Reagents and sample preparation: Prepare enzyme solutions, buffers, samples to be enzymatically hydrolyzed and other reagents. Ensure that the quality of all reagents meets experimental requirements and is correctly placed in the designated location on the workstation. 3. Pipetting operation: The mechanical arm of the workstation moves the suction head to the top of the enzyme solution container to accurately absorb the set amount of enzyme solution. The robotic arm moves the tip over the sample to be enzymatically hydrolyzed and accurately releases the enzyme solution into the sample. Depending on experimental needs, the workstation may require multiple pipetting operations to ensure that the enzyme solution is evenly distributed among multiple samples. 4. Enzymatic hydrolysis reaction control: After adding the enzyme solution, the workstation can cooperate with other laboratory equipment (such as a constant temperature oscillator) to control the temperature, oscillation frequency and other conditions of the enzymatic hydrolysis reaction. These conditions are crucial to the rate and efficiency of the enzymatic hydrolysis reaction, and the workstation ensures the smooth progress of the enzymatic hydrolysis reaction by precisely controlling these factors. 5. Data recording and analysis: Workstations are usually equipped with a data recording system that can automatically record key parameters such as pipetting volume and pipetting time. After the experiment, researchers can use these data to further analyze and optimize the enzymatic hydrolysis reaction.

The fully automatic pipetting workstation provides unprecedented convenience and accuracy for enzymatic hydrolysis experiments with its excellent liquid control capabilities, efficient automated operations, and seamless connection with a variety of laboratory equipment. By precisely controlling the pipetting volume, optimizing the pipetting path, and cooperating with other equipment, the fully automatic pipetting workstation not only significantly improves the efficiency and consistency of enzymatic hydrolysis experiments, but also provides scientific researchers with a more reliable and repeatable method. Experimental results.

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