Application of fully automatic pipetting workstation and C18 chromatographic column in HPLC

The fully automatic pipetting workstation and the C18 chromatographic column each play an important role in high performance liquid chromatography (HPLC) analysis. The hydrophilic and hydrophobic characteristics of the C18 chromatographic column can effectively separate compounds of different polarities. When combined with a fully automatic pipetting workstation, the sample injection process is highly automated, improving the repeatability and stability of experiments.

Fully automatic pipetting workstation

1. The role of the fully automatic pipetting workstation 1. Accurate measurement and distribution: The fully automatic pipetting workstation can accurately measure and distribute samples and solvents, ensuring consistent sample amounts for each analysis and reducing human operation errors. 2. Automated operation: Through programming and preset programs, the fully automatic pipetting workstation can automatically complete sample preparation, dilution, mixing and injection steps to improve work efficiency. 3. Reduce contamination: The fully automatic pipetting workstation uses a closed pipetting system, which reduces the contact between samples and solvents and the external environment, reducing the risk of contamination.

2. The role of C18 chromatographic column 1. Compound separation: C18 chromatographic column is a key part of the HPLC system. It uses octadecylsilane bonding groups on the silica matrix to separate compounds in the sample. 2. High analytical column efficiency: C18 chromatographic columns usually have high column efficiency and can achieve accurate separation and quantitative analysis of complex samples. 3. Wide scope of application: C18 chromatographic columns are suitable for the separation of various types of biological macromolecules and organic compounds, and are widely used in food, chemical industry, bioindustry, pharmaceutical and other fields.

3. Cooperation between the fully automatic pipetting workstation and C18 chromatographic column 1. Sample preparation and injection: In HPLC analysis, the fully automatic pipetting workstation is responsible for sample preparation and injection. It accurately measures and distributes the sample and then injects it into the injector of the HPLC system to prepare it for separation on the C18 column. 2. Mobile phase preparation and delivery: The fully automatic pipetting workstation can also be used for the preparation and delivery of mobile phase. It precisely formulates and mixes different proportions of solvents to form the required mobile phase and delivers it to the C18 column through the high-pressure pump of the HPLC system. 3. Data analysis and result output: Although the fully automatic pipetting workstation itself does not directly participate in data analysis and result output, it provides a reliable foundation for subsequent chromatographic data analysis and result output through precise liquid transfer and distribution. . 4. Improve work efficiency and accuracy: The cooperation between the fully automatic pipetting workstation and the C18 chromatographic column makes the entire HPLC analysis process more automated and efficient. It reduces human operation errors and inconveniences and improves the accuracy and reliability of analysis.

The fully automatic pipetting workstation and the C18 chromatographic column play their own unique roles in HPLC analysis, and achieve precise separation and analysis of samples through close cooperation. This cooperation not only improves work efficiency and accuracy, but also provides strong support for scientific research and technology development.

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