In modern molecular biology, polymerase chain reaction (PCR) technology has become an indispensable tool. It not only can quickly amplify trace amounts of DNA, but also provides important support for genetic analysis, disease diagnosis and genetic research. The emergence of PCR fully automatic pipetting workstations makes the processing of PCR experiments more efficient and precise. It uses automated partition processing to significantly improve the accuracy and reliability of experiments.
PCR fully automatic pipetting workstation
1. Preparation stage 1. Cleaning and disinfection: Thoroughly clean and disinfect the PCR automatic pipetting workstation and its surrounding environment to reduce the risk of cross-contamination. Use an appropriate disinfectant and make sure the disinfectant is compatible with the experimental materials. 2. Reagents and sample preparation: Prepare the required PCR reagents (such as primers, template DNA, enzymes, etc.) and samples according to experimental needs. Ensure the quality and purity of reagents and samples and avoid using expired or contaminated reagents. 3. Workstation setup: Place the PCR fully automatic pipetting workstation on a stable table and connect the power cord. Power on the workstation and wait for it to start up and load the appropriate control software. Set experimental parameters in the software, including sample number, pipetting volume, temperature cycle parameters, etc.
2. Sample processing and reagent distribution 1. Sample loading: Place the sample to be processed in the designated location of the PCR fully automatic pipetting workstation according to the experimental requirements. Use the sample processing module of the workstation to preprocess samples, such as dilution, extraction, etc. 2. Reagent distribution: According to the experimental parameters, the PCR fully automatic pipetting workstation will automatically distribute the required reagents into the corresponding reaction tubes or 96-well plates. During the dispensing process, the workstation will precisely control the movement of the pipetting head and the amount of liquid dispensed to ensure the accuracy of the experiment.
3. PCR amplification 1. Reaction system preparation: Under the control of the workstation, mix the sample and reagents into the reaction system. Ensure that the proportions of components in the reaction system are accurate and consistent. 2. Temperature cycle: The PCR fully automatic pipetting workstation will perform the amplification reaction according to the preset temperature cycle parameters. During the temperature cycle, the workstation will accurately control the operation of the heating block and temperature sensor to ensure that the reaction system is amplified at the correct temperature. 3. Collection of amplification products: After the PCR amplification is completed, the workstation will automatically collect the amplification products. During the collection process, the workstation ensures that the purity and concentration of the product meet the requirements for subsequent analysis.
4. Product analysis (optional) If the PCR fully automatic pipetting workstation integrates the product analysis function, then product analysis can be performed on the workstation. 1. Product detection: Use the detection module integrated in the workstation to detect the amplification product. During the detection process, the workstation will accurately control the operation of the detection instrument and output the detection results. 2. Data analysis: Analyze the data output by the workstation based on the detection results. During the analysis process, professional data analysis software can be used to calculate and evaluate parameters such as amplification curves and CT values.
5. Post-experiment processing 1. Equipment cleaning and maintenance: After completing the experiment, thoroughly clean and maintain the PCR automatic pipetting workstation. During cleaning, be careful not to use cleaning agents or tools that may cause damage to the equipment. 2. Experiment record and summary: record the experimental process and results in detail. Summarize and evaluate the experimental results to provide reference for subsequent experiments.
The PCR fully automatic pipetting workstation can complete the partitioning operation process in one stop. During the entire experimental process, the workstation can accurately control the operation of each step to ensure the accuracy and reliability of the experiment. At the same time, the high degree of automation of the workstation also greatly improves experimental efficiency and reduces manual operation errors and contamination risks.
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