How to calibrate the OT-2 peptide desalting workstation

The OT-2 peptide desalting workstation is a piece of equipment widely used in biochemistry and molecular biology research. In order to ensure the accuracy and reliability of experimental results, regular calibration is crucial. This article details how to effectively calibrate the OT-2 Peptide Desalting Workstation.

OT-2 peptide desalting workstation

1. Preparation before calibration 1. Check the equipment: Make sure that the OT-2 workstation is in good working condition and has no obvious damage or failure. 2. Prepare calibration tools: According to the Opentrons calibration guide, prepare the required calibration tools and materials, such as calibration probes, calibration plates, etc. 3. Connect the device: Connect the OT-2 workstation to the computer or mobile device to ensure that the Opentrons application can be accessed.

2. Calibration steps 1. Start the calibration program: Open the Opentrons application and connect to the OT-2 workstation. Select the "Calibrate" option in the application to enter the calibration procedure. 2. Calibrate the probe: Install the calibration probe according to the Opentrons calibration guide. Select the "Probe Calibration" option in the app and follow the prompts to calibrate. During calibration, care is taken to ensure accurate alignment of the probe with the calibration plate or other reference point. 3. Calibrate the deck: Deck calibration is a key step to ensure that the experimental equipment is correctly placed on the OT-2 workstation. Select the "Deck Calibration" option in the app and follow the prompts to calibrate. During the calibration process, you need to ensure that each slot on the deck corresponds exactly to its location in the application. 4. Calibrate the pipette: The calibration of the pipette is crucial to the accuracy of the experiment. Select the "Pipette Calibration" option in the app and follow the prompts to calibrate. During the calibration process, it may be necessary to adjust parameters such as the pipette tip length and offset. 5. Run calibration check: After completing the above calibration steps, run a calibration check program. Checking procedures will simulate actual experimental operations to verify the accuracy of the calibration. If any problems or errors are found, recalibration will be required.

3. Precautions after calibration 1. Record calibration data: Record the data and results obtained during the calibration process for future reference and comparison. 2. Regular calibration: In order to ensure the continued accuracy of the OT-2 workstation, it is recommended to perform regular calibration. The specific calibration frequency can be determined based on experimental needs and equipment usage. 3. Maintain the equipment: During use, pay attention to keeping the OT-2 workstation clean and tidy to avoid dust and dirt from causing damage to the equipment. At the same time, regularly check whether all parts of the equipment are operating normally, and repair or replace them in time if there are any problems.

Accurate calibration of the Opentrons OT-2 peptide desalting workstation is the key to ensuring reliable experimental data and improving work efficiency. From the precise alignment of the probe to the stable support of the deck, to the fine adjustment of the pipette, every step of calibration carries the pursuit of scientific research rigor and persistence in experimental accuracy. By following detailed calibration steps, we can not only effectively avoid operational errors, but also improve the purity and efficiency of peptide desalting, laying a solid foundation for the smooth advancement of scientific research projects.

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