Flex应用专题 | 解锁蛋白质谱前处理自动化的无限潜能

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Automated sample extraction with Opentrons Flex workstation

With its excellent automated sample extraction capabilities, Opentrons Flex workstation is gradually becoming an indispensable core tool in the fields of life sciences, clinical diagnosis, and drug development. With its innovative modular design, high-precision pipetting technology and intelligent software platform, the Opentrons Flex workstation can automatically perform complex processes from sample pretreatment to target substance extraction, greatly simplifying experimental steps and improving work efficiency. , and ensure the accuracy and reliability of experimental results.

Opentrons Flex工作站的自动化样本提取

1. Advantages of automated sample extraction 1. Improve experimental efficiency: Automated processing can significantly reduce the manual operation time of experimental personnel, increase experimental throughput, and thereby speed up the experimental process. 2. Reduce human errors: By accurately controlling pipetting, mixing, incubation and other steps, errors introduced by human factors are avoided and the accuracy and reliability of experimental results are improved. 3. Enhance the repeatability of experiments: The standardized automated process ensures the consistency of each experiment and enhances the repeatability of the experiment. 4. Reduce experimental costs: Automated processing reduces the waste of reagents and consumables and reduces experimental costs.

2. Opentrons Flex automated sample extraction process 1. Sample preparation: Place the sample to be extracted in an appropriate container and perform preprocessing (such as lysis, dilution, etc.) according to experimental requirements. 2. Reagent configuration: According to the requirements of the extraction method, configure the corresponding reagents (such as lysis solution, washing solution, magnetic beads, etc.). 3. Program setting: Set the extraction program on the touch screen of Opentrons Flex, including pipetting steps, temperature control, magnetic stand operation, etc. 4. Execute extraction: Place the sample and reagents into the workstation, and start the program for automated extraction. The workstation will automatically complete pipetting, mixing, incubation, washing, separation and other steps according to the preset program. 5. Target substance purification: remove impurities and purify the target substance through specific purification steps (such as magnetic bead adsorption, centrifugation, etc.). 6. Data recording and analysis: Record key parameters and results during the entire extraction process, and perform data analysis to evaluate the reliability and stability of the extraction method.

3. Features of Opentrons Flex automated sample extraction 1. High-precision pipetting technology Opentrons Flex automated pipetting workstation has high-precision and accurate pipetting functions, which can ensure the precise ratio of samples and reagents, thereby ensuring the reliability of experimental results. Through flexible pipetting channel configurations (such as 1/8/96 channels), Opentrons Flex can meet the throughput requirements of different experiments while reducing experimental errors. 2. Modular design Opentrons Flex adopts a modular design, and users can select and configure corresponding functional modules according to their own experimental needs. These modules include but are not limited to temperature control modules, magnetic stands, oscillators, turn plate grippers, etc. They work together to provide comprehensive support for automated sample extraction. For example, the magnetic stand can be used for magnetic bead separation and purification in magnetic bead extraction; the temperature control module can accurately control the reaction temperature to ensure stability during sample processing. 3. Intelligent operation Opentrons Flex is equipped with a 7-inch LCD smart screen. Users can directly touch the screen to operate Protocol settings and operations, one-click equipment calibration, process monitoring and other tasks. This makes the operation more intuitive and convenient, while also improving the automation and repeatability of the experiment. Users can program and customize protocols to implement specific automated sample extraction processes to meet diverse experimental needs.

The automated sample extraction function of Opentrons Flex workstation not only greatly improves laboratory work efficiency, but also provides solid technical support for scientific research and clinical diagnosis by reducing human errors and enhancing experimental repeatability. Its modular and scalable design concept enables the Opentrons Flex workstation to adapt to the individual needs of different laboratories, thereby achieving more flexible and efficient automation solutions.

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