In order to reduce manual errors in the reagent dispensing process and free up the labor force of laboratory personnel, a fully automatic reagent dispensing workstation came into being. This article will introduce in detail the workflow of the Opentrons Flex reagent dispensing workstation, starting from the early equipment configuration and consumable preparation stages, to the meticulous execution process of reagent dispensing, to the later data processing and equipment maintenance, comprehensively demonstrating how this workstation can improve It plays an integral role in experimental efficiency, ensuring data accuracy, and optimizing the use of experimental resources.
Opentrons Flex Reagent Dispensing Workstation
1. Preliminary preparation 1. Equipment configuration: According to the experimental needs, select the appropriate pipette configuration, such as Flex 8-channel pipette or Flex 96-channel pipette, to meet the processing needs of different sample volumes. Configure other necessary modules, such as thermal cycle module, temperature module, thermal oscillation module and ring magnetic stand, to support various operations in the experiment. 2. Preparation of consumables: Prepare enough pipettes, reagent bottles, reaction plates and other consumables, and ensure that they meet the experimental requirements. Properly install filaments on the Flex workstation to ensure stability and ease of operation. 3. Experimental design: Find or create a suitable experimental protocol in Opentrons’ online protocol library. According to the experimental protocol, set the relevant parameters of the Flex workstation, such as pipetting volume, pipetting speed, temperature, etc.
2. Reagent dispensing process 1. Sample loading: Place the sample to be dispensed at the designated location on the Flex workstation to ensure that the sample is clearly labeled and easy to identify. 2. Reagent addition: The Flex workstation automatically draws the required amount of reagent from the reagent bottle according to the preset protocol. Dispense reagents into designated reaction plates or sample tubes through precise pipetting. 3. Mixing and incubation (if necessary): For samples that need to be mixed or incubated, the Flex workstation will automatically perform the corresponding operations. Use a thermal cycler module or temperature module for temperature control to ensure your sample is reacting under the appropriate conditions. 4. Quality testing (if necessary): During or after packaging, relevant testing instruments can be used to conduct quality testing on the samples. According to the test results, adjust the packaging parameters or perform other necessary operations in a timely manner.
3. Post-processing 1. Data recording and analysis: The Flex workstation will automatically record various parameters and results during the dispensing process. Scientific researchers can use relevant data analysis software to further analyze and process the recorded data. 2. Recycling and processing of consumables: After the packaging is completed, the used consumables should be recycled and disposed of in a timely manner to ensure the cleanliness and safety of the laboratory. 3. Equipment cleaning and maintenance: Clean and maintain the Flex workstation regularly to ensure it is in good working condition. Inspect and replace worn parts to extend the life of your equipment.
4. Precautions 1. During the entire work process, the laboratory's safety operating procedures should be strictly followed. 2. Regularly calibrate and verify the Flex workstation to ensure its accuracy and reliability. 3. Select appropriate reagents and consumables according to experimental needs to avoid waste and pollution.
From the above description of the workflow, it can be seen that the Opentrons Flex reagent dispensing workstation has a high degree of automation and flexibility, which can greatly improve the efficiency and quality of laboratory work. At the same time, its open source software platform and rich online protocol library also provide researchers with more choices and convenience.
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