NGS library preparation refers to the process of converting target DNA or RNA fragments into a form that can be read by sequencing instruments. This usually involves breaking DNA or RNA into fragments of appropriate sizes, performing end repairs, adapter ligation, purification, and amplification, and ultimately generates a collection containing a large number of small DNA or RNA fragments, a library. This library can be loaded directly onto a sequencer for high-throughput sequencing.
Opentrons Flex NGS Library Preparation Workstation
1. Functions of NGS library preparation 1. Support high-throughput sequencing: The main function of NGS library preparation is to provide high-quality sequencing samples for high-throughput sequencing. Through the prepared libraries, the sequencer can efficiently read a large amount of genetic information to conduct subsequent genomics research, transcriptomic research, and research on disease-related gene variations. 2. Improve sequencing efficiency: Prepared libraries can significantly improve sequencing efficiency. By optimizing various steps in the library preparation process, such as improving DNA fragmentation efficiency and optimizing adapter connection conditions, it can be ensured that the sequencer can accurately read the sequence information of each fragment, thereby improving the accuracy and reliability of sequencing. 3. Reduce sequencing costs: NGS library preparation technology can also reduce sequencing costs to a certain extent. By optimizing library preparation methods and improving sequencing efficiency, the cost of reagents and instruments required for sequencing can be reduced, thereby reducing the overall sequencing cost. 4. Support diverse research: Prepared NGS libraries can be used for various types of research. For example, it can be used in different types of sequencing projects such as genome re-sequencing, transcriptome sequencing, and epigenome sequencing to meet the needs of researchers for analyzing different types of genetic information. 5. Promote scientific research progress: The development of NGS library preparation technology has promoted scientific research progress. Through high-throughput sequencing technology, researchers can gain a deeper understanding of the genetic characteristics, gene expression patterns and disease mechanisms of organisms, thereby providing new ideas and methods for disease treatment, drug development and other fields.
2. Opentrons Flex NGS library preparation 1. Full-process automated library preparation The Opentrons Flex NGS library preparation workstation has the ability to automate the entire library construction process and can meet the sample and library preparation needs in NGS and PCR applications. This includes reagent distribution, magnetic bead processing, and general biochemistry and genome transfer, which can significantly reduce the time required for the entire process and the labor intensity of manual operations. 2. Efficiently process high-throughput samples. This workstation is equipped with Flex pipettes and corresponding consumables, as well as a variety of modules, such as thermal cycle module, temperature module, thermal oscillation module and ring magnetic stand. These multiplex modules operate cooperatively, allowing the sample processing throughput to be as high as 96, and can efficiently complete the library preparation process of 96 samples. 3. Compatible with a variety of reagents and protocols. Opentrons Flex NGS library preparation workstation has passed multiple reagent adaptation tests and can be expanded to adapt to all reagent protocols. This means that laboratories can choose appropriate reagents for library preparation according to their own needs without worrying about compatibility issues. 4. Precise pipetting and quality control ensure that this workstation has precise pipetting capabilities and strict quality control. It undergoes advanced inspection before leaving the factory to ensure pipetting accuracy and pass rate. This helps ensure the quality and consistency of library preparations. 5. Graphical operation and programming support Opentrons Flex NGS library preparation workstation provides a graphical operation interface and supports Python programming, which makes the operation more convenient and flexible. Users can customize programming according to experimental needs to achieve more complex library preparation processes. 6. Supports multiple library preparation types. This workstation not only supports amplicon library construction, but can also be used for other types of library preparation, such as genome libraries, transcriptome libraries, etc. This enables the Opentrons Flex NGS library preparation workstation to meet a variety of experimental needs.
Opentrons Flex NGS workstation greatly reduces the manual operation burden in the library preparation process. At the same time, it significantly improves the accuracy and repeatability of experiments while ensuring quality control and accuracy. Whether it is amplicon library construction or other types of library preparation needs, Opentrons Flex NGS workstation can fully meet the diverse needs of laboratories with its excellent performance.
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