Opentrons Flex magnetic bead protein purification workstation combines the advantages of magnetic bead technology and efficient automated workstations, making the protein purification process simpler, faster and more stable. This article will introduce in detail the basic process of the Opentrons Flex magnetic bead protein purification workstation and its significant advantages in practical applications, helping you to deeply understand the function and value of this equipment.
Opentrons Flex Magnetic Bead Protein Purification Workstation
1. Core parameters and functional modules 1. Core parameters: (1) Pipetting platform: It has 11 plates + 1 garbage chute, and can process up to 96 samples at a time. (2) Pipette: single-channel/8-channel fixed-spacing pipette, pipetting range 1~1000ul, pipette CV value is low, ensuring pipetting accuracy. (3) Temperature control module: The temperature range is 4~95°C, with high temperature accuracy and uniformity to meet different experimental needs. (4) Thermal cycle module: The temperature range is wider, the module base temperature range is 499°C, and the module upper cover temperature range is 37110°C. (5) Magnetic bead purification module: It uses high-strength lift-type N52 neodymium magnets and is compatible with standard and deep-hole laboratory equipment and various magnetic bead products from 22mm to 44mm. 2. Function modules: (1) Pipetting platform: used to automate pipetting operations and improve work efficiency. (2) Temperature control module: used to control the experimental temperature to ensure stable experimental conditions. (3) Thermal cycle module: realizes rapid temperature rise and fall to meet specific experimental needs. (4) Magnetic bead purification module: Use magnetic bead separation technology to achieve protein purification.
2. Operation process 1. Sample preparation: Prepare samples that need to be purified, such as cell lysate, etc., and ensure their purity and stability. Perform pretreatment according to experimental needs to increase the concentration of target protein. 2. Magnetic bead addition: The system automatically adds magnetic beads to the sample. The surface of the magnetic beads is modified with specific ligands and can capture the target protein. Ensure that the magnetic beads and sample are evenly mixed to improve binding efficiency. 3. Mixing and incubation: Use the automatic mixing function to promote the binding of magnetic beads to target proteins. Set the right mixing time and speed without manual intervention. Incubation at specific temperatures can further improve binding. 4. Magnetic separation: Use a built-in magnetic stand for separation. The magnetic beads are adsorbed on the tube wall, and impurities and non-specific proteins remain in the supernatant. The system accurately removes the supernatant to ensure high protein purity and recovery. 5. Washing step: Add washing solution and mix briefly to remove non-specific binding impurities. Wash several times to ensure complete removal of impurities. 6. Protein elution: Add eluent to dissociate the target protein from the surface of the magnetic beads. The system automatically completes the eluent addition and centrifugation collection steps to obtain high-purity target proteins.
3. Advantages of Opentrons Flex magnetic bead protein purification 1. Efficient automation: significantly reduce manual intervention and reduce the risk of operational errors. Automatically perform steps such as loading, mixing, separation and elution. 2. Strong flexibility: compatible with a variety of magnetic bead types and protein separation protocols. Flexible to adapt to different research needs, it is widely used in drug research and development, molecular biology research and other fields. 3. High repeatability: Strictly control the sample volume and time to ensure consistent results for each operation. Reduce inter-laboratory differences and improve the credibility of experimental results. 4. High-throughput processing: Process multiple samples at one time to improve experimental efficiency. Save time and labor costs, suitable for large-scale protein production and screening experiments.
Opentrons Flex magnetic bead protein purification workstation provides researchers with convenient and accurate protein purification solutions with its comprehensive automation design, flexible operation capabilities and efficient magnetic bead purification technology. It plays an important role in many fields, improving the efficiency of protein purification and ensuring high consistency and high-throughput processing capabilities of purification.
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