In the fields of molecular biology research and biopharmaceuticals, protein purification is a crucial link. Opentrons Flex magnetic bead protein purification workstation greatly improves the efficiency and accuracy of protein purification through automation and intelligence. However, to ensure continued high-precision operation of the workstation, regular calibration is essential.
Opentrons Flex Magnetic Bead Protein Purification Workstation
1. Calibration steps The calibration of the Opentrons Flex magnetic bead protein purification workstation usually includes multiple aspects, such as probe installation calibration, thermal oscillation module calibration, temperature control module calibration, and thermal cycle module calibration. The following is a general calibration step: 1. Probe installation calibration: Make sure the probe is correctly installed on the workstation and check whether its connection is firm. Calibrate the probe using standard materials or calibration tools according to the calibration guide to ensure it can pipette accurately. 2. Thermal oscillation module calibration: Set a specific temperature and oscillation frequency, and use a standard thermometer and oscillation frequency meter to calibrate the thermal oscillation module. Adjust module parameters until preset accuracy and stability are achieved. 3. Temperature control module calibration: Use a high-precision thermometer to calibrate the temperature control module to ensure that it can accurately control the temperature of the reaction system. Based on the calibration results, adjust the parameters of the temperature control module to achieve the required temperature accuracy. 4. Thermal cycle module calibration: Set a specific thermal cycle program, including heating, cooling, and heat preservation stages. Calibrate the thermal cycler module using a high-precision thermometer to ensure it can accurately control temperature according to the preset program. Based on the calibration results, adjust the parameters of the thermal cycler module to achieve the desired temperature accuracy and stability.
2. Calibration Precautions 1. Follow the calibration guide: Before performing calibration, be sure to carefully read and follow the calibration guide of the Opentrons Flex Magnetic Bead Protein Purification Workstation. 2. Use standard materials: Certified standard materials or calibration tools should be used during the calibration process to ensure the accuracy and reliability of the calibration results. 3. Record calibration data: After each calibration, the calibration data should be recorded in detail, including calibration time, calibration results and any necessary adjustment measures. 4. Regular calibration: In order to ensure the continuous high-precision operation of the workstation, it is recommended to perform regular calibration. The specific calibration frequency should be determined based on the use of the workstation and experimental requirements.
3. Verification after calibration After completing the calibration, the Opentrons Flex magnetic bead protein purification workstation should be verified to ensure that it can accurately perform protein purification operations. Validation can include purification using protein samples of known concentrations and comparing the purification results to expected values.
Calibration of the Opentrons Flex magnetic bead protein purification workstation is an important step to ensure its accuracy and reliability. By following calibration guidelines, using standard materials, recording calibration data, and performing regular calibrations, you can ensure high-precision operation of your workstation, providing strong support for molecular biology research and biopharmaceutical fields.
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