Features and functions of NGS laboratory workstations

The NGS (Next Generation Sequencing) laboratory workstation is an automated work platform specially designed for high-throughput sequencing technology. It integrates a variety of functional modules, including sample preprocessing, library construction, PCR amplification, and sequencing preparation. and (in some cases) steps such as data analysis, designed to improve experimental efficiency, reduce human error, and ensure the accuracy and reproducibility of experimental results.

1. Main features of NGS laboratory workstation

1. High degree of automation:

1>Automated sample processing: From sample aliquots, nucleic acid extraction, library construction to pre-sequencing preparation, all automated operations can be performed unattended or with minimal manual intervention.

2>Automated data analysis: Some workstations also integrate data analysis software, which can automatically process sequencing data and improve analysis efficiency and accuracy.

2. High precision and consistency:

1> Ensure the stability and consistency of each experimental result through sophisticated instruments and optimized experimental procedures.

2>Reduce errors and contamination risks caused by human operations.

3. Efficiency:

1>Significantly improve experimental throughput and support simultaneous processing of multiple samples.

2>Shorten the experimental cycle and improve the overall laboratory work efficiency.

4. Flexibility:

1>Workstations usually support a variety of NGS reagents and protocols to meet different experimental needs.

2>Can be customized and upgraded according to the specific conditions of the laboratory.

2. Main functions of NGS laboratory workstation:

1. Sample pre-processing:

1>Automatically complete the packaging, labeling and storage of samples to reduce human operating errors.

2>Automated DNA/RNA extraction, using efficient extraction technologies such as magnetic bead method and silica gel membrane method to ensure the purity and integrity of nucleic acids.

3> Sample quality control, evaluate nucleic acid quality through fluorescence quantification, electrophoresis and other methods to ensure the smooth progress of subsequent experiments.

2. Library construction:

1>Automatically complete DNA/RNA fragmentation, end repair, A-tail addition, adapter connection and other steps to construct a sequencing library.

2>Supports a variety of library construction solutions, such as PCR-free libraries, single-stranded libraries, etc., to meet the needs of different sequencing platforms.

3>Library quality control, evaluate library quality through qPCR, library size detection and other methods to ensure the validity and uniformity of the library.

3. Target sequence capture (optional):

1>For experiments that require targeted sequencing, the workstation can automatically complete the capture and enrichment of target sequences.

2>Supports multiple capture technologies, such as hybridization capture, PCR amplification capture, etc., to improve the pertinence and depth of sequencing.

4. PCR amplification:

1>Automatically perform library amplification to ensure that the DNA/RNA molecules in the library reach the concentration required for sequencing.

2>Controllable PCR cycle number and temperature conditions to reduce amplification deviation and non-specific amplification.

5. Preparation before sequencing:

1>Purify the amplified library to remove unreacted primers, dNTPs and other impurities.

2>Quantitatively detect the library concentration to ensure the consistency of the sample loading for each sequencing channel.

3>Load the library into the sequencing chip or flow cell and prepare for sequencing.

6. Data analysis support (some workstations):

1> Some NGS laboratory workstations integrate data analysis software or interfaces, which can automatically process sequencing data and conduct preliminary data quality control, comparison, mutation detection and other analyses.

2> Provide intuitive data visualization tools to help researchers quickly understand experimental results.

7. Automated management:

1> Equipped with advanced automated control systems and software, it can automatically execute preset experimental processes and reduce manual intervention.

2>Support multi-task parallel processing to improve the overall work efficiency of the laboratory.

3> Provide experimental records and data analysis reports to facilitate laboratory management and tracking of scientific research projects.

3. Construction requirements

The construction of NGS laboratory workstations needs to meet certain standards and requirements, including:

1. Laboratory environment: You must have a standard NGS laboratory that meets relevant regulations to ensure a sterile and dust-free environment in the laboratory.

2. Equipment configuration: Equipped with high-throughput sequencer and supporting servers, automatic analysis software, laboratory sample information management system and other necessary equipment.

3. Personnel training: Testing personnel must pass specialized skills training and obtain corresponding employment certificates.

4. Management system: Establish strict laboratory management system, standardized operating procedures (SOP) and quality management documents to ensure that the daily operation of the laboratory meets the requirements of the National Ministry of Health.

4. Summary

The NGS laboratory workstation is an important support platform for high-throughput sequencing technology. It provides strong support for clinical disease screening, diagnosis and scientific research through its high degree of automation, high precision and efficiency. With the continuous advancement of technology and the expansion of applications, NGS laboratory workstations will play an important role in more fields.