Application of fully automatic pipetting workstation in protein labeling experiments

Protein labeling experiments are a common type of experiment in biological and medical research, aiming to track, detect and quantitatively analyze proteins by introducing specific markers (such as isotopes, fluorescent dyes, etc.). Such experiments usually require high precision, high throughput, and a high degree of automation to ensure the accuracy and reliability of experimental results. The fully automated pipetting workstation is the ideal tool to meet these needs.

Fully automatic pipetting workstation

1. Working principle of the fully automatic pipetting workstation The fully automatic pipetting workstation realizes the automated operation of liquid pipetting by combining computers and precision machinery. Its core components include pipetting tips, pump heads, control systems and working environments. When working, the computer uses image processing technology to identify each well position on the experimental plate and calculate the optimal pipetting path. The pump head precisely controls the movement of the pipette tip and the liquid aspiration according to the instructions of the computer. At the same time, the system will also perform quality testing on the absorbed liquid, such as volume error, concentration error, etc., to ensure the accuracy and consistency of pipetting.

2. Application of the fully automatic pipetting workstation in protein labeling experiments 1. Precisely control the pipetting volume: The fully automatic pipetting workstation can accurately control the pipetting volume, thereby ensuring that the concentration of the marker in each sample is consistent in the protein labeling experiment. . This is critical for accuracy and reproducibility of experiments. 2. High-throughput processing capability: This workstation has high-throughput processing capability and can process multiple samples at the same time. This is particularly important for protein labeling experiments that require large amounts of samples and can significantly improve experimental efficiency. 3. Automated operation: The fully automatic pipetting workstation can automatically complete steps such as pipetting, mixing, incubation, washing and detection, thereby reducing manual operation errors and human interference. This makes the experimental results more reliable and stable. 4. Compatible with a variety of reagents and consumables: This workstation is compatible with a variety of common magnetic bead-based reagents and supports a variety of workflows. This allows users to flexibly select reagents and workflows based on experimental needs, further improving the flexibility and applicability of experiments.

3. Specific experimental steps and operations In protein labeling experiments, the specific operating steps of the fully automatic pipetting workstation are as follows: 1. Sample preparation: Mix the protein sample to be labeled with an appropriate amount of marker. 2. Pipetting and mixing: Use a fully automatic pipetting workstation to accurately distribute the mixture into each well on the experimental plate and perform a mixing operation. 3. Incubation: Place the experimental plate at an appropriate temperature and incubate it to fully combine the marker with the protein. 4. Washing and purification: Use a fully automatic pipetting workstation to wash and purify the labeled proteins to remove unbound labels and impurities. 5. Detection and analysis: Use appropriate detection methods to detect and analyze the labeled proteins and draw experimental conclusions.

The fully automatic pipetting workstation has significant advantages and broad application prospects in protein labeling experiments. Its high precision, high throughput, and high degree of automation make this workstation excellent in handling complex and time-consuming protein labeling experiments. For example, the technology of our company's fully automatic pipetting workstation Opentrons Flex magnetic bead protein purification workstation is very mature and has been successfully used in multiple protein labeling experiments. It is the best choice for laboratories carrying out protein labeling experiments or other high-throughput experiments. .

Heating oscillation module introduction

Extract genomic DNA from cell or tissue samples using Opentrons Flex™ Nucleic Acid Extraction Workstation Purification

User Manual: Opentrons Single Channel Pipette

The embodiment of fully automatic pipetting workstation in robotic technology

What toxicity testing projects is the fully automatic pipetting workstation suitable for?

Introduction to the entire desalting process of OT-2 peptide desalting workstation