High-throughput screening platform

The high-throughput screening platform is a comprehensive scientific research platform that integrates cutting-edge technology and advanced equipment. Its original design is to achieve high-efficiency, high-speed, high-sensitivity and high-accuracy operations to analyze massive compound libraries or complex compounds. Systematic screening and in-depth analysis of biological sample sets. This platform not only accelerates the process of scientific research, but also greatly improves the efficiency and depth of exploration in areas such as drug discovery, gene function analysis, and biomarker identification.

1. Technical Principles of High-Throughput Screening Platform The high-throughput screening platform is based on experimental methods at the molecular level and cellular level, using microplate format as the experimental tool carrier, executing the experimental process through an automated operating system, and using sensitive and fast detection instruments. Collect experimental result data, and finally analyze and process the experimental data by computer. The platform can detect tens of millions of samples at the same time and operate with the corresponding database support, greatly improving the efficiency of drug discovery and biochemical research.

2. How to use the high-throughput screening platform 1. Preliminary preparation (1) Determine the screening goals: clarify the types of compounds, targets or disease models to be screened, and the expected experimental results. (2) Construct a compound library: Based on the screening goals, construct a library containing a large number of potentially active compounds. These compounds can come from natural products, synthetic compound libraries, or existing drug libraries. (3) Prepare experimental materials: including microplates, consumables required for automated operating systems, reagents required for detection instruments, etc. 2. Experimental design (1) Design screening model: According to the screening goal, select an appropriate screening model, such as target-based screening model, phenotype-based screening model, etc. (2) Determine experimental conditions: including sample concentration, reaction time, temperature, pH value, etc., to ensure the accuracy and repeatability of experimental results. (3) Plan the experimental process: Use the automated operating system to plan the experimental process, including the sample addition sequence, reaction steps, detection steps, etc. 3. Experimental operations (1) Sample addition: Use an automated operating system to add samples from the compound library to the microwell plate according to certain rules. (2) Reaction process: Place the microplate under appropriate conditions for reaction, such as incubation, centrifugation, etc. (3) Result detection: Use sensitive and fast detection instruments to collect experimental results, such as liquid scintillation counters, chemiluminescence detectors, spectrophotometers, etc. to detect the biological activity of compounds. 4. Data analysis (1) Data collection: Collect the data obtained by the detection instrument and import it into the computer for processing. (2) Data preprocessing: Clean and organize the original data to remove outliers and noise data. (3) Statistical analysis: Use statistical methods to analyze the data and identify compounds with potential biological activity. (4) Result verification: further verify the selected active compounds, such as confirming their biological activity and safety through cell experiments, animal experiments, etc. 5. Follow-up research (1) Structure-activity relationship analysis: Analyze the structure of active compounds, explore the relationship between their structure and activity, and provide guidance for drug design. (2) Drug development: Further develop potential active compounds as drug candidates, including drug synthesis, structure optimization, pharmacokinetic studies, etc.

3. Application of high-throughput screening platform 1. Drug discovery: High-throughput screening platform is an important tool in the drug discovery process and can accelerate the screening and optimization process of candidate drugs. 2. Biochemical research: In the field of biochemistry, high-throughput screening platforms can be used to study biological processes such as interactions of biomolecules and changes in enzyme activity. 3. Disease diagnosis and treatment: Through high-throughput screening technology, disease-related biomarkers can be discovered, providing new ideas and methods for disease diagnosis and treatment.

The technical principle of the high-throughput screening platform is based on experimental methods at the molecular level and cellular level, using automated operating systems and sensitive and fast detection instruments to quickly, sensitively and accurately screen and analyze a large number of samples. This technology has the characteristics of multi-disciplinary interdisciplinary, high degree of automation and strong data analysis capabilities, and has broad application prospects in many fields.