As a new generation of automated sample processing platform, Flex NGS workstation not only greatly improves the efficiency and accuracy of library construction, but also provides users with unprecedented experimental flexibility and scalability. Regarding the configuration recommendations for the Flex NGS workstation, the following is a configuration plan based on high efficiency and flexibility to meet the needs of small and medium-throughput library construction:
1. Core components 1. Flex Robot: This is the core of the Flex NGS workstation, responsible for automated pipetting and other operations. 2. Pipette: It is recommended to configure single-channel, eight-channel and 96-channel pipettes to meet different sample volumes and experimental needs. Choose from two Flex 8-channel pipettes, suitable for processing up to 48 samples, or a single Flex 96-channel pipette, capable of handling up to 96 samples. 3. Suction tips: Suction tips used with pipettes to ensure accurate and pollution-free pipetting operations.
2. Function module 1. Thermal cycle module: used for steps such as PCR amplification that require temperature control. 2. Magnetic module: used for magnetic bead separation and other steps to improve the efficiency and purity of library preparation. 3. Temperature control module: Provides precise temperature control to ensure the stability and reliability of the experimental process. 4. The Flex ring magnetic stand is made of permanent magnet material, which can provide a stable and powerful magnetic field to ensure the effective separation of magnetic particles.
3. Experimental plate positions and consumables 1. Experimental plate positions: The Flex NGS workstation is recommended to be configured with 12+4 standard plate positions, which can accommodate a variety of experimental plates and consumables. 2. Consumables: including but not limited to PCR plates, deep well plates, tip boxes, etc. to ensure the smooth progress of the experiment.
4. Software and protocols 1. Opentrons Protocol protocol library: Contains verified scripts and protocols, allowing users to download and use them directly to improve work efficiency. 2. Customized Protocol: Users can customize the experimental process and steps according to their own experimental needs.
5. Other configuration suggestions 1. Workstation layout: According to the experimental process and needs, rationally plan the workstation layout to improve operational efficiency. 2. Compatibility: Ensure that the Flex NGS workstation can be used with any library construction kit on the market to improve the flexibility and versatility of experiments. 3. Maintenance and maintenance: Regularly clean, calibrate and maintain the Flex NGS workstation to ensure long-term stable operation of the equipment.
The configuration of the Flex NGS workstation should comprehensively consider core components, functional modules, experimental platforms and consumables, software and protocols, and other configuration suggestions. Through reasonable configuration and planning, the efficiency and flexibility of the Flex NGS workstation can be fully utilized to meet the needs of small and medium-throughput library construction.
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