Electronic pipette usage tutorial

Electric pipettes are the new standard for high-precision, high-quality pipetting in the laboratory. It is intuitive and easy to operate, has high pipetting accuracy and repeatability, and can help you easily complete long-term high-throughput pipetting and complex liquid dispensing work. It provides a variety of commonly used pipetting methods, uses a direct charging system, and does not require a charging stand. It is a truly multi-functional product.

1. Principle of electric pipette The working principle of electric pipette is that the piston realizes liquid aspiration and discharge through the telescopic movement of spring. Under the push of the piston, part of the air is discharged, the liquid is sucked in using atmospheric pressure, and then the piston pushes the air to discharge the liquid. When using an electric pipette, the speed and force of pipetting can be well controlled by operating with the elasticity of the spring.

2. Product features: · It conforms to ergonomic mechanics and uses special materials, allowing you to easily "lift" multi-channel pipettes with one hand. · A symbol of laboratory automation and standardization, designed for high-throughput, multi-sample situations! It is convenient for you to operate samples on a large scale, with accurate measuring range and accurate measurement at one time. You no longer need to worry about too many samples. · Good air-tight design, perfectly integrated with the suction head, you don’t need to worry about air leakage or looseness of the suction head. · The pipette holder occupies a small space and can be placed in a safety cabinet to maximize your experimental space. · The detachable dispensing head (lower part of multi-channel pipette) can be sterilized by high temperature and pressure. · The powerful memory function makes it easy to repeat the setting procedure of the last experiment, and is designed to solve problems in complex operations. · Efficient after-sales service, professional response service within 8 hours, solving after-sales problems for you without worries.

3. Operation steps of electric pipette: 1. First, use a wiper paper dipped in a small amount of 75% alcohol to wipe the surface of the electric large-capacity pipette; 2. Choose a pipette of appropriate size, open the package from the top, and only touch it with your hands The part above the graduation line; 3. Correctly insert the pipette into the cone connector of the electric large-capacity pipette, and then remove the remaining pipette packaging; 4. Open the lid of the container to be pipetted and keep the pipette inserted vertically. into the liquid surface, press the aspiration button to aspirate the liquid; 5. Use the graduated mark on the pipe wall to measure the volume of liquid to be transferred; 6. After the aspiration is completed, release the aspiration button and rub it on the inner wall of the test tube. rub the pipette, and then Lift the pipette out of the container; 7. Hold the pipette upright and stick it to the inner wall of the container, press the discharge button, and carefully release the liquid; 8. After the liquid transfer is completed, remove the pipette correctly and handle it properly. Special reminder: It is very undesirable to use the electric pipette to repeatedly hit the tip to tighten it. If you do this for a long time, the parts in the electric pipette will become loose due to strong impact, and the electric pipette may even cause The knob for adjusting the scale is stuck.

●Tips for pipetting 1) Pre-moisten the aspiration electric pipette. Viscous liquids can be pipetted by pre-moistening the tip. First aspirate the sample liquid and then pump it out. A layer of liquid will be adsorbed on the inner wall of the tip. The surface adsorption reaches saturation, and then the sample liquid is inhaled, and the volume of the final liquid pumped out will be very large.

2) Forward suction and reverse suction: Forward suction refers to the normal suction method. When suctioning, press the button to the position to aspirate and release the button. When discharging liquid, press the first gear to expel most of the liquid, and then press the second gear to drain the remaining liquid. Reverse suction means that when suctioning, press the button directly to the second position and then release it. This will suck in more liquid. When dispensing liquid, just press the first position. The more liquid sucked in can compensate for the surface adsorption inside the tip. Reverse pipetting is generally used in combination with pre-wetting liquid pipetting. Electric pipettes are suitable for viscous liquids and volatile liquids.

●Common operating errors: When assembling the tip, repeatedly hit the tip with the electric pipette. Tighten correctly: Insert the tip, turn left and right to tighten the tip. Error: The tip does not match the electric pipette. Affects the air tightness Correct: Choose a quality-assured tip that matches the electric pipette. Error: When aspirating, tilt the electric pipette to aspirate. Correct: Electric pipette Vertical aspiration error: When the tip contains undischarged liquid, the electric pipette is placed flat on the table. Correct: Hang the electric pipette vertically on the electric pipette holder. Incorrect: Use an electric pipette with a large capacity to pipette. Correct for taking a small volume of liquid: The pipetting volume must be within the range provided by the electric pipette to meet the requirements of inaccuracy and inaccuracy. Wrong: Correct for taking a highly volatile liquid: If highly volatile liquids must be pipetted, the electric pipette should be disassembled immediately after pipetting to allow the steam to evaporate. At the same time, it is recommended to use an external piston electric pipette. Error: The aspiration and dispensing speeds are too fast. Correct: Electric pipette, aspirate slowly and release slowly.