Proteomics sequencing sample requirements vary depending on the experimental purpose, sample type, and subsequent analysis needs. The following are some general sample requirements, but please note that specific requirements may need to be adjusted based on the laboratory's specific protocol and technique used:
Proteomics sequencing sample requirements
1. Sample quality Proteomics sequencing covers a wide range of sample types, including but not limited to cells, tissues, body fluids (such as blood, urine, cerebrospinal fluid, etc.), microorganisms, animal and plant tissues, and environmental samples (such as soil, water, air, etc.) . 1. Purity and integrity: Samples should be as pure as possible to avoid interference from impurities and contaminants. For tissue samples, irrelevant tissues such as blood vessels, fat, and connective tissue need to be removed to ensure the representativeness of the sample. 2. Freshness: Freshly collected samples should be processed as soon as possible to prevent protein degradation and inactivation. Samples that cannot be processed immediately should be properly stored, such as quick-frozen in liquid nitrogen and stored in a -80°C refrigerator. 3. Avoid repeated freezing and thawing: Samples should avoid repeated freezing and thawing to reduce protein degradation and denaturation. If repackaging is required, it should be quickly frozen in liquid nitrogen, repackaged, and used as soon as possible.
2. Sample size Sample size varies depending on the purpose of the experiment and the type of sample. Generally speaking, the following are some common sample size requirements: 1. Cells: For proteomics projects, approximately 1 × 107 cells are usually required. 2. Tissue: Animal tissue samples usually require a fresh weight of about 100mg (proteomics project) or 500mg (phosphoproteomics project). Plant tissue samples require more fresh weight, such as 200mg (proteomics project) or 1g (phosphoproteomics project). 3. Body fluids: such as blood, urine, etc., a sufficient amount of samples should be collected according to experimental needs, and care should be taken to avoid hemolysis, contamination and other problems.
3. Sample processing and preservation 1. Cleaning and removal of impurities: For cell and tissue samples, it is necessary to use pre-cooled PBS and other buffers for cleaning to remove culture medium residues, blood and other contaminants. 2. Lysis and extraction: Use appropriate lysis solutions and extraction methods to release the proteins in the sample. For tissue samples, steps such as homogenization and centrifugation may also be required. 3. Storage and transportation: Samples should be stored in a -80°C refrigerator. If transportation is required, low-temperature transportation methods such as dry ice should be used. For some special samples, such as strips, protein solutions, etc., special attention must be paid to storage and transportation conditions.
4. Other precautions 1. Avoid keratin contamination: When handling samples, protective equipment such as gloves, masks, and headgear should be worn to avoid contamination by keratin and other contaminants. 2. Sample labeling and recording: Clearly label and record samples, including sample type, collection time, processing steps and other information for subsequent analysis and tracking. 3. Follow laboratory regulations: During sample processing, laboratory regulations and operating procedures should be strictly followed to ensure the accuracy and safety of the experiment.
Proteomics sequencing sample requirements involve many aspects, including sample type, quality, quantity, processing and storage, etc. In order to ensure the accuracy and reliability of the experiment, the collection, processing and storage of samples should be carried out in strict accordance with the regulations and requirements of the laboratory.
Related reading recommendations
Heating oscillation module introduction
Opentrons8 channel GEN2 electronic pipette
Introduction to Opentrons Flex™ Ring Magnetic Module
What robotic technology is used in the OT-2 PCR workstation
Factors affecting Opentrons Flex PCR workstation data
Tip specifications of OT-2 nucleic acid extraction workstation
The experienced service team and strong production support team provide customers with worry-free order services.
