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The 96-channel fully automatic pipetting workstation has 96 independent pipetting channels and can handle multiple samples or reagents at the same time. Its operation process includes preparation, setting parameters, installing pipetting heads, performing pipetting operations, cleaning and maintenance, etc. step. The following will introduce the detailed operation process of the 96-channel fully automatic pipetting workstation:
1. Preparation work
1. Placement and connection: Place the 96-channel fully automatic pipetting workstation on a horizontal platform and turn on the power.
2. Initialization: Turn on the instrument switch and wait for the instrument initialization to complete. During the initialization process, the instrument will perform self-tests to ensure that all components are working properly.
2. Set parameters
1. Program selection: On the instrument operation interface, select the name of the first-level program to be executed, and enter the number of cycles and cycle interval of the first-level program.
2. Parameter adjustment: According to the experimental needs, set the pipetting workstation’s aspiration volume, discharge volume, aspiration speed and other parameters. These parameters can be set via buttons on the instrument panel, touch screen or software interface.
3. Install the pipetting head
1. Select the pipetting head: Select the appropriate pipetting head according to the experimental needs, and ensure that the specifications and model of the pipetting head match the experimental requirements.
2. Install the pipetting head: Install the selected pipetting head on the suction head of the pipetting workstation, ensuring that the pipetting head is installed firmly to avoid loosening or falling off during operation.
4. Perform pipetting operations
1. Aspiration operation: Insert the pipetting head into the container of the liquid to be aspirated, press the aspiration button, and release the button after the liquid is aspirated into the pipetting head. Pay attention to controlling the suction speed to avoid bubbles or liquid splashing.
2. Discharge operation: Insert the pipetting head into the target container, press the discharge button, and release the button after the liquid is discharged into the target container. It is also necessary to pay attention to controlling the discharge speed to ensure that the liquid is discharged accurately and evenly into the target container.
5. Cleaning and maintenance
1. Clean the pipetting head: After each use, use cleaning solution to clean the pipetting head to ensure that the pipetting head is clean and free of contamination. This helps extend the life of the pipetting head and prevents cross-contamination.
2. Maintenance: Clean and maintain the pipetting workstation regularly, including cleaning the surface of the instrument, checking the connection of each component, and replacing damaged parts. This helps keep the instrument in good condition and ensures the accuracy and reliability of your experiments.
6. Precautions
1. Safe use: When using the pipetting workstation, be careful to avoid contact with chemical reagents or biological samples to avoid danger or contamination. At the same time, laboratory safety regulations and operating procedures should be followed.
2. Operating specifications: During operation, the pipetting head should be inserted into the liquid gently to avoid bubbles or liquid splashing. At the same time, the procedures and parameters set by the program should be strictly followed to ensure the accuracy and reliability of the experiment.
This article introduces the operation process of a general 96-channel fully automatic pipetting workstation. It is only used as a reference for readers to understand the operation process of the 96-channel fully automatic pipetting workstation. Different brands and models of pipetting workstations may have different operating details, so in actual operations, you should refer to the instruction manual of the specific instrument for operation.
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