In precision laboratory operations, the pipette is the core tool for liquid transfer, and the selection of its specifications is directly related to the accuracy and efficiency of the experiment. Whether it is micro-analysis or large-scale sample processing, pipettes have become an indispensable assistant for scientific researchers because of their precise and controllable operating characteristics.
1. Specifications of the pipette gun 1. Measuring range The 12-channel pipette gun has a wide range of measuring ranges, ranging from ultra-micro volume to conventional measuring range. Common measuring ranges include: (1) 0.5-10ul: suitable for experiments that require precise transfer of trace amounts of liquid. (2) 2-20ul: It is also suitable for the transfer of trace liquids, but the range is slightly larger. (3) 5-50ul: Suitable for medium-range liquid transfer. (4) 10-100ul: Suitable for larger range of micro-liquid transfer. (5) 20-200ul: The measuring range has been further expanded and is suitable for a variety of experimental needs. (6) 50-300ul: Suitable for experiments that require the transfer of larger amounts of liquid. 2. Other performance parameters (1) Repeatability: refers to the consistency when using a pipette to transfer the same volume of liquid multiple times under the same conditions. A pipette with good repeatability can ensure the stability and reliability of experimental results. (2) Resolution: refers to the smallest volume change that the pipette can recognize. The high-resolution pipette can adjust the liquid volume more accurately to meet the experimental requirements for trace amounts of liquid. (3) Applicability: Different brands and models of 12-channel pipettes may be suitable for different experimental environments and sample types. Therefore, when choosing a pipette, you need to consider whether it is suitable for your experimental needs.
2. Accuracy and Error of Pipette Gun 1. Precision The accuracy of pipette gun usually refers to its ability to accurately transfer liquid in actual operation. The higher the accuracy, the smaller the deviation of the pipette when transferring the same volume of liquid repeatedly. However, it should be noted that the accuracy is not absolute, but has a certain range of fluctuations. 2. Error The error of the pipette gun refers to the deviation between the volume of liquid transferred by the pipette gun and the set volume in actual operation. Errors may originate from a variety of factors, such as temperature fluctuations, poor air tightness, reagent volatilization, improper operation, etc. Therefore, when using a pipette, it is necessary to minimize the influence of these factors to improve the accuracy and reliability of the experiment. 2. Specific values. The specific values of the accuracy and error of the pipette gun will vary according to different brands, models and measuring ranges. Generally speaking, for the same brand and model of pipetting gun, the smaller the measuring range, the higher the accuracy and error. For example: (1) For a pipette gun with a measuring range of 10ul, the allowable error range is generally within ±0.1ul. (2) For a pipette gun with a measuring range of 1000ul, the allowable error range may be within ±10ul. These values are for reference only. In actual use, the specific accuracy and error range need to be determined according to the instructions or calibration report of the pipette.
12-channel pipette guns come in a variety of specifications, covering a variety of options from micro-volume to large-volume. At the same time, the accuracy and error also vary according to different brands, models and ranges. When selecting a 12-channel pipette, laboratory personnel should comprehensively consider factors such as specific experimental needs, sample type, and operating habits.
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