In life science research, nucleic acid extraction is an important step in obtaining genetic information, and it is also a key link in promoting scientific research progress. With the increasing experimental needs and the pursuit of accuracy, the OT-2 nucleic acid extraction workstation stands out in many laboratories with its high degree of automation and modular design. Especially when OT-2 is equipped with a thermal oscillation module, this combination significantly improves the efficiency and accuracy of nucleic acid extraction.
OT-2 Nucleic Acid Extraction Workstation
1. Overview of OT-2 Nucleic Acid Extraction Workstation The OT-2 Nucleic Acid Extraction Workstation is designed based on the principle of fully automatic magnetic bead extraction and can automate the nucleic acid separation and purification workflow. The workstation includes core components such as the OT-2 automated pipetting platform, high-precision pipettes, magnetic bead purification modules, and optional thermal shaking modules. By integrating these modules, the OT-2 nucleic acid extraction workstation can process up to 24 samples at a time, greatly saving experimental time and labor costs.
2. The function and function of the thermal oscillation module The thermal oscillation module is one of the optional modules of the OT-2 nucleic acid extraction workstation. It provides deck heating and microplate orbital oscillation and shaking functions. Specifically, the thermal oscillation module has the following functions and effects: 1. Heating function: The thermal oscillation module can set and maintain a certain temperature range (such as 37°C to 95°C) to meet the requirements for sample incubation during the nucleic acid extraction process. Constant temperature conditions. By heating, the enzymatic reaction in the sample can be promoted and the efficiency of nucleic acid extraction can be improved. 2. Oscillation function: The thermal oscillation module also has an orbital oscillation function, and its vibration range is usually between 200-3000rpm. By shaking, the sample can be evenly mixed in the microwell plate, improving the binding efficiency of nucleic acids and magnetic beads, thereby further improving the purity of nucleic acid extraction.
3. Application of thermal oscillation module in nucleic acid extraction In the process of nucleic acid extraction, the application of thermal oscillation module is mainly reflected in the following aspects: 1. Sample incubation: After adding the extraction reagent, the sample needs to be incubated at a certain temperature for a period of time. time to promote the release of nucleic acids and binding to magnetic beads. The heating function of the thermal shaking module can accurately set and maintain the required temperature to ensure the smooth progress of the incubation process. 2. Magnetic bead binding: In the magnetic bead purification step, the sample and magnetic beads need to be mixed and oscillated to promote the binding of nucleic acids and magnetic beads. The oscillation function of the thermal oscillation module can achieve this goal and improve the binding efficiency of magnetic beads and nucleic acids. 3. Washing and elution: During the magnetic bead purification process, multiple washings are required to remove unbound impurities. The oscillation function of the thermal oscillation module can help the washing solution better contact and mix with the magnetic beads, thereby improving the washing effect. At the same time, during the elution step, the oscillation function of the thermal oscillation module can also promote the separation of the eluent and the nucleic acid bound to the magnetic beads.
When the OT-2 nucleic acid extraction workstation is equipped with a thermal oscillation module, it can significantly improve the efficiency and accuracy of nucleic acid extraction experiments. By combining heating and shaking functions, the thermal shaking module can optimize steps such as sample incubation, magnetic bead binding, washing and elution, thereby improving the purity and efficiency of nucleic acid extraction. Therefore, for scientific research institutions and enterprises that need to conduct a large number of nucleic acid extraction experiments, the optional thermal oscillation module of the OT-2 nucleic acid extraction workstation is a choice worth considering.
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