In the vast field of life science research, the development of experimental equipment is rapidly affecting the way scientists explore. High-throughput, high-precision and automated equipment, especially the OT-2 NGS workstation, is becoming an important tool for scientific research. Due to its excellent performance, flexible configuration and diverse applications, this workstation has attracted much attention in many laboratories. This article will delve into the core components of the OT-2 NGS workstation and analyze in detail what key equipment is required to create an efficient and comprehensive NGS experimental platform.
1. Core equipment: OT-2 pipetting robot 1. Equipment introduction: OT-2 pipetting robot is a high-precision, high-throughput, cost-effective and highly customizable pipetting equipment developed by Opentrons Labworks Group. 2. Functional features: Able to complete precise pipetting work from 1µL to 1000µL. The 96-well plate can be filled within 22 seconds, improving experimental efficiency. It can be used with a variety of modules, such as single-channel pipette, 8-channel pipette, temperature control module, thermal cycle module, magnetic module and HEPA module to further expand experimental functions.
2. Supporting modules 1. Single-channel/8-channel pipette: Adapted to the OT-2 pipetting robot, available in single-channel and 8-channel specifications to meet different experimental needs. 2. Temperature control module: used to control the temperature during the experiment to ensure stable experimental conditions. 3. Thermal cycle module: used to perform experiments such as PCR steps that require temperature cycling. 4. Magnetic module: used to perform experimental steps such as magnetic bead cleaning, and used in conjunction with the OT-2 pipetting robot to improve experimental efficiency. 5. HEPA module: Provides air purification function to ensure the cleanliness of the experimental environment.
3. Other necessary equipment 1. SBS standard experimental consumables: such as test tubes, pipettes, centrifuge tubes, etc., used to store and transfer experimental samples. 2. Computer and software: used to control the OT-2 pipetting robot, collect and analyze experimental data. Opentrons provides a complete software solution that allows users to easily set experimental parameters, monitor experimental progress and analyze results through a computer.
4. Workstation configuration recommendations 1. Space layout: Ensure that the OT-2 pipetting robot and its supporting modules have enough space for installation and operation. At the same time, taking into account the operational convenience and safety of experimental personnel, the spatial layout of the workstation should be reasonably designed. 2. Power supply and network connection: Provide stable power supply and network connection for the OT-2 pipetting robot and its supporting equipment to ensure the smooth progress of the experimental process. 3. Safety protection measures: Set necessary safety protection measures in the workstation, such as protective glasses, gloves, etc., to ensure the safety of experimental personnel.
The core equipment required for the OT-2 NGS workstation is the OT-2 pipetting robot and its supporting modules (single-channel/8-channel pipette, temperature control module, thermal cycle module, magnetic module and HEPA module). At the same time, necessary equipment such as SBS standard experimental consumables, computers and software are also required. When configuring workstations, you should fully consider issues such as space layout, power and network connections, and security protection measures.
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