The fully automatic pipetting workstation can significantly improve the efficiency and accuracy of experiments when performing BCA (Bicinchoninic Acid) and Bradford detection. These two detection methods are commonly used protein quantification techniques in biochemistry and molecular biology experiments. The introduction of fully automatic pipetting workstations makes these detection processes more automated, efficient and reliable.
Fully automatic pipetting workstation
1. BCA detection BCA detection is a method for measuring protein concentration based on the principle that protein reduces Cu2+ to Cu+ under alkaline conditions and forms a purple complex with BCA reagent. The fully automatic pipetting workstation can automatically complete the following steps when performing BCA detection: 1. Reagent preparation: The workstation uses a high-precision pump and sensor to accurately measure BCA reagents A and B (or A, B, C, according to the specific kit) depending) and mix evenly to form a working reagent. 2. Sample processing: Add the protein sample to be tested into a 96-well plate (or other suitable container), and the workstation automatically removes an appropriate amount of working reagent, adds it to each well, and mixes it. 3. Incubation and detection: Incubate the mixed sample at an appropriate temperature for a certain period of time to allow the protein to fully react with the BCA reagent. After the incubation, the workstation automatically transfers the sample to a plate reader or spectrophotometer and measures the absorbance at a specific wavelength to calculate the protein concentration.
2. Bradford test Bradford test uses the principle of color change after Coomassie brilliant blue dye binds to protein to measure protein concentration. The fully automatic pipetting workstation can also automatically complete steps such as reagent preparation, sample processing, incubation and detection when performing Bradford detection. Different from the BCA test, the dyes and reaction conditions used in the Bradford test are different: 1. Dye preparation: The workstation accurately measures the Coomassie Brilliant Blue dye solution and prepares the appropriate buffer. 2. Sample processing: Add the protein sample to be tested into the container, and the workstation automatically removes an appropriate amount of dye solution and buffer, adds it to each sample, and mixes it. 3. Incubation and detection: Incubate at room temperature for a certain period of time to fully combine the dye with the protein. After the incubation, the workstation automatically transfers the sample to a plate reader or spectrophotometer and measures the absorbance at a specific wavelength to calculate the protein concentration.
3. Advantages of the fully automatic pipetting workstation 1. Improve experimental efficiency: The fully automatic pipetting workstation can automatically complete steps such as reagent preparation, sample processing, incubation and detection, significantly improving experimental efficiency. 2. Improve experimental accuracy: The workstation is equipped with high-precision pumps and sensors, which can accurately control the pipetting volume and reaction conditions, thus improving the accuracy of the experiment. 3. Reduce human error: Fully automatic operation reduces the chance of manual intervention, thereby reducing the risk of human error. 4. Easy to standardize: The workstation can automatically execute the preset experimental process, making the experimental results easier to standardize and compare.
The fully automatic pipetting workstation has significant advantages when performing BCA and Bradford detection, and can significantly improve the efficiency and accuracy of experiments. In biochemistry and molecular biology experiments, fully automatic pipetting workstations have become one of the indispensable automation tools.
Related reading recommendations
OT-2 Liquid Handling Robot Quick Start Guide
User Manual: OT-2 Liquid Handler
Heating and shaking module GEN1 Quick Start Guide
How to perform ELISA processing on the fully automatic pipetting workstation
Factors affecting Opentrons Flex PCR workstation data
How to maintain the Opentrons Flex reagent dispensing workstation
The experienced service team and strong production support team provide customers with worry-free order services.
