Flex应用专题 | 解锁蛋白质谱前处理自动化的无限潜能
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Pipettes are a broader concept that includes many types of liquid pipetting tools that may differ in design and function, but all have in common the ability to pipet and dispense liquids. Pipettes usually have high-precision adjustment and volume control, and are suitable for experiments that require strict control of pipetting volume. A pipette is a specific type of pipette. It is usually designed to be relatively simple and is mainly used for rapid and large-volume liquid transfer.
1. Design and function 1. Pipette: complex design, with high-precision adjustment and range control. There are various types, including single-channel pipettes, multi-channel pipettes (such as 8-channel, 12-channel, etc.) and electronic pipettes. It is suitable for experiments that require high precision and repeatability, such as PCR, enzyme labeling reactions, quantitative analysis, and operations in various laboratories such as life sciences, chemistry, and medical research. 2. Pipetting gun: The design is relatively simple and is mainly used for fast and large-volume pipetting operations. It is divided into handheld pipette gun and electric pipette gun. Electric pipette gun improves operating efficiency and precision through electric control. It is suitable for pipetting large volumes of liquid (such as more than 10 mL), such as solution preparation, culture medium dispensing, etc., as well as scenarios that require rapid and large-scale pipetting operations.
2. Operation mode 1. Pipette: Provides two operation modes: manual and electronic. Manual pipettes pipette liquids using manually adjustable volume and piston mechanisms. Electronic pipettes integrate electronic control and display screens, provide multiple functions and high precision, are suitable for complex and highly repetitive experimental operations, and can reduce human errors and improve efficiency. 2. Pipetting gun: It also provides two operating modes: manual and electric. The manual pipette gun manually controls the aspiration and discharge speed, which is simple to operate, but the accuracy is relatively low. The electric pipette gun controls liquid aspiration and discharge through an electric pump, which improves operating efficiency and precision, while paying more attention to operating speed and convenience.
3. Precision and Repeatability 1. Pipette: It has high precision and repeatability, and is suitable for experiments that require strict control of pipetting volume. High-precision, high-quality pipettes are relatively expensive, but they can meet the application scenarios of high-throughput screening and strict experimental requirements. 2. Pipetting gun: The accuracy and repeatability are relatively low, but the speed is faster and the operation is more convenient. Suitable for scenarios where pipetting accuracy is not so strict.
4. Use and maintenance 1. Pipette: It needs to be cleaned and disinfected before use, and the operating manual must be fully understood. When using, you need to control the volume of the pipette to ensure accurate pipetting, and calibrate the pipette before each operation. After use, maintenance work such as cleaning and disinfection is required, and proper storage is required. During long-term maintenance, it is necessary to maintain and inspect all parts of the pipette to ensure that it is in good condition. 2. Pipette gun: When using it, you also need to pay attention to cleaning and disinfection, as well as correct operation methods. However, due to its relatively simple design, the complexity of maintenance and upkeep is relatively low.
A pipette is a specific form or type of pipette, while a pipette is a broader concept that includes a variety of liquid pipetting tools including pipettes. When choosing which tool to use, experimenters should comprehensively consider factors such as specific experimental needs, ease of operation, accuracy requirements, and budget.
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