Basic steps and methods of liquid workstation programming

The programming of a liquid workstation is a comprehensive process that combines complex steps and deep professional knowledge. It not only covers the fine operations of software programming, but also involves the precise debugging of hardware settings, as well as the continuous optimization and adjustment of experimental processes. This process is designed to maximize the potential of liquid workstations through the power of programming to meet the laboratory's stringent requirements for efficiency, precision and automation.

1. Software programming 1. Program design and writing (1) Set the mixing program: First, you need to set the mixing program of the liquid workstation through software programming, including the steps and parameters of liquid absorption, transfer, and mixing. This usually includes setting the movement path of the pipetting head, the volume of liquid to be aspirated, the target position of the transferred liquid, etc. (2) Multi-channel settings (if applicable): If the liquid workstation has multi-channel capabilities, programs can be written to operate multiple samples at the same time to improve experimental efficiency. (3) Time control: Set the execution time of each step to ensure that the entire mixing process proceeds according to the predetermined time node. 2. Parameter adjustment and optimization (1) Speed ​​and amplitude settings: Adjust the speed and amplitude of the pipetting head during the mixing process according to experimental requirements to avoid liquid splashing or bubbles. (2) Time control refinement: Refine the mixing time according to experimental needs to ensure that two or more liquids are fully mixed. 3. Monitoring and feedback (1) Monitor the mixing effect: Monitor various parameters during the mixing process through software, such as liquid status, mixing uniformity, etc., and make adjustments if necessary. (2) Set alarm program: Set alarm conditions in the program, such as abnormal liquid level, equipment failure, etc., so that problems can be discovered and dealt with in time.

2. Hardware setup and debugging 1. Preparation for instrument operation (1) Check the status of the instrument: Make sure the liquid workstation is in good working condition and all components are tightly connected and leak-free. (2) Install the pipetting head: Select the appropriate pipetting head according to the experimental requirements and install it in the designated position. 2. Instrument displacement display and adjustment (1) Real-time display: Real-time display of the current instrument displacement through the software interface for monitoring and adjustment. (2) Manual adjustment: When necessary, the position or angle of the pipetting head can be manually adjusted to achieve the best mixing effect. 3. Serial port connection settings (1) Adjust serial port parameters: Set the correct serial port parameters, such as baud rate, data bits, stop bits, etc., to ensure normal communication between the instrument and the computer. (2) Connection test: Carry out serial port connection test to ensure that the instrument can respond to computer instructions and execute correctly.

3. Experimental process optimization 1. Experimental steps optimization and simplification process: Simplify unnecessary steps according to experimental needs and improve experimental efficiency. Standardized operations: Develop standardized operating procedures and parameter setting methods to ensure the accuracy and repeatability of experimental results. 2. Data analysis and feedback Data analysis: Conduct detailed analysis of experimental data to evaluate mixing effects and other related parameters. Feedback adjustment: Adjust program parameters and experimental processes based on analysis results to optimize experimental results. 3. Documentation and archiving of the experimental process: record the various parameters and operating steps during the experiment in detail for subsequent review and reproduction of the experiment. Archive files: Archive and save experimental reports, program codes, data files, etc. for subsequent use or review.

Liquid workstation programming is a comprehensive process that requires comprehensive consideration of software programming, hardware setup and debugging, and experimental process optimization. Through reasonable steps and methods, it can be ensured that the liquid workstation can accurately and efficiently perform mixing tasks according to predetermined procedures.