High-precision pipetting system

As an indispensable core tool in modern laboratories, high-precision pipetting systems, with their unparalleled accuracy, excellent stability and highly automated operating characteristics, lead scientific researchers to deeply explore the microscopic fields of life sciences and help drugs Breakthroughs have been made in many key areas such as research and development, genetic engineering, protein analysis, and environmental monitoring.

1. Definition and characteristics A high-precision pipetting system is an automated device that can accurately control and transfer trace amounts of liquid. It combines advanced liquid handling technology, high-precision sensors and automated control systems to achieve precise control of liquid volume and high-efficiency transfer. Its main features include: 1. High precision: Using non-contact sonic pipetting technology or high-precision robotic arm technology, it can achieve high-precision liquid transfer from nanoliters to microliters with extremely low errors. 2. High speed: supports high-throughput operation and can process multiple samples at the same time, significantly improving experimental efficiency. 3. High repeatability: The precise control system and stable mechanical structure ensure good repeatability of each pipetting operation. 4. Automation: Integrated intelligent operating system supports multiple pipetting modes and program settings. Users can customize the operation process according to actual needs to achieve automated operations.

2. Technical principles The technical principles of high-precision pipetting systems are diverse, but they are mainly based on the following two types: 1. Acoustic pipetting technology: The energy field generated by sound waves is used to accurately transfer liquid from the source container to the target container. This technology uses a dynamic liquid analysis algorithm to accurately determine the liquid composition and liquid height in the mother plate, and calculate the energy required to transfer the precise volume of liquid to the target plate. The entire process requires no physical contact and avoids the risk of cross-contamination. 2. High-precision robotic arm technology: Using a precise robotic arm structure, combined with high-precision sensors and control systems, to achieve precise control of liquid volume. The robotic arm can complete the suction, transfer and release operations of liquids with micron-level precision.

3. Application of high-precision pipetting system in Opentrons equipment 1. Product overview of Opentrons OT-2 automated pipetting platform Opentrons OT-2 is a desktop-level automated pipetting platform with high precision, high flexibility and high cost performance. Features. The platform adopts a modular design and can be configured according to experimental needs to meet the needs of a variety of experimental scenarios. High-precision pipetting system applications (1) High-precision pipettes: The OT-2 platform is equipped with Opentrons-specific high-precision pipettes, such as PIPETTE GEN2. This pipette can achieve precise pipetting in the range of 1uL to 1000μL, and its pipetting accuracy is comparable to pipetting equipment that is ten times more expensive on the market. When absorbing 1 ul of liquid, the accuracy CV value is ±5%, ensuring the accuracy of experimental results. (2) Multi-channel configuration: The OT-2 platform can be equipped with both single-channel and 8-channel pipettes to meet the throughput requirements of different experiments. This flexibility allows the OT-2 to handle a variety of experimental scenarios from low-throughput to high-throughput. (3) Automated process: Through Opentrons’ Protocol API (Python-based programming interface), researchers can write customized experimental programs to automate the experimental process. This programming method greatly lowers the threshold for automated experiments, and complex experimental processes can be created without professional knowledge. 2. Opentrons Flex fully automatic pipetting workstation Product Overview Opentrons Flex is the third generation fully automatic pipetting workstation developed by Opentrons, which represents a new technological breakthrough in the field of laboratory automation. This workstation has the characteristics of high flexibility, wide applicability and high throughput, and can better adapt to the experimental process. High-precision pipetting system applications (1) 96-channel high-precision pipetting: Flex pipette uses air displacement technology and has 96-channel high-precision pipetting capabilities, which can perform high-precision pipetting of 1-1000 μL. The pipette is equipped with smart sensors with automatic calibration, real-time positioning and error detection functions to ensure the accuracy and stability of pipetting. (2) Fully automatic turn plate gripper: Flex's turn plate gripper is installed on the Z-axis gantry, which can grip laboratory instruments and move the turn plate on modules such as magnetic bead separation plates or thermal cyclers to achieve high efficiency. Turn plate operation. (3) Intelligent graphic prompts: Flex is equipped with a 7-inch LCD touch screen system, which is made of anti-scratch and anti-damage LCD material. The software system has an intelligent graphic prompt function, making the experimental operation easier and easier to understand.

We have witnessed how high-precision pipetting systems, with their excellent performance, have helped scientific researchers achieve a series of impressive results in fields such as biomedicine, chemical analysis, genomics, proteomics, and environmental monitoring. From the precise control of tiny molecules to the automated execution of complex experiments, high-precision pipetting systems provide solid technical support for scientific research and exploration with their irreplaceable advantages.