High-throughput sequencing pipetting

Faced with tens of thousands of samples and complex and ever-changing experimental processes, traditional manual pipetting methods are not only time-consuming and labor-intensive, but also more difficult to avoid errors caused by human factors. It is against this background that the high-throughput sequencing pipetting solution emerged. It uses the power of technology to transform tedious pipetting operations into a series of accurate and automated processes, greatly improving experimental efficiency and Reliability of results.

1. The importance of pipetting for high-throughput sequencing In high-throughput sequencing, the accuracy and stability of pipetting operations are crucial to the accuracy of subsequent sequencing results. Since high-throughput sequencing requires processing a large amount of samples and reagents, traditional manual pipetting methods are not only inefficient but also prone to human error. Therefore, the use of automated equipment such as high-throughput sequencing pipetting workstations to complete pipetting operations has become a standard feature in modern sequencing laboratories. Opentrons Flex fully automatic pipetting workstation also provides high-throughput sequencing pipetting solutions, saving valuable time and resources for scientific researchers and making scientific experiments more efficient and flexible.

2. High-throughput sequencing pipetting capabilities 1. High-precision pipetting: Flex pipette uses air displacement technology and can perform high-precision pipetting of 1-1000 μL. The pipette is equipped with smart sensors with automatic calibration, real-time positioning and error detection functions to ensure the accuracy and stability of pipetting operations. 2. High-throughput configuration: Flex’s Z-axis gantry can install any two single-channel or 8-channel pipettes, or configure a Flex 96-channel pipette separately. This configuration enables the Flex fully automated pipetting workstation to handle a large number of samples and reagents to meet the needs of high-throughput sequencing. 3. Multifunctional modules: The Flex workstation is also equipped with a variety of functional modules, such as thermal cycle module, temperature module, thermal oscillation module and ring magnetic stand. These modules can work together to further improve the efficiency and efficiency of high-throughput sequencing pipetting. accuracy.

3. Advantages and characteristics of high-throughput sequencing pipetting 1. Open source ecology: Opentrons provides open source hardware and software platforms. Users can customize settings and develop according to their own needs, making the Flex workstation more flexible and easy to integrate. 2. AI empowerment: Opentrons Flex workstation supports AI-empowered laboratory automation. Through the online protocol library and Python API open source, scientific researchers can quickly develop and apply new experimental protocols. 3. High compatibility: Flex workstation is compatible with ANSI/SLAS standard orifice plates and other commonly used laboratory equipment on the market, including conical reservoirs and test tubes up to 50 mL.

4. Application Scenarios Opentrons Flex fully automatic pipetting workstation has been widely used in the field of high-throughput sequencing. For example, during the library preparation process, by combining QIAseq® FX DNA Library Kit and QIAseq® Normalizer Kit and other reagents, the Flex workstation can automatically complete the library preparation process for 96 samples. The entire process only takes 4-5 hours and requires manual labor. Operation time is less than 1 hour. This efficient and precise pipetting capability provides a strong guarantee for the smooth conduct of high-throughput sequencing experiments.

With the continuous deepening of life science research and the rapid development of technology, Opentrons high-throughput sequencing pipetting solutions are gradually becoming a new benchmark for laboratory automation. It not only greatly improves the efficiency and accuracy of high-throughput sequencing experiments, but also provides researchers with unprecedented flexibility and convenience.