Sample preprocessing workstation

The sample pretreatment workstation is an important equipment in the laboratory for preprocessing biological, chemical or environmental samples. This workstation integrates a variety of functional modules designed to improve the efficiency and accuracy of sample preprocessing, thereby providing high-quality samples for subsequent analysis or experiments.

1. Sample pre-processing workflow

1. Sample collection

• Purpose: To obtain the samples required for analysis from the source.
• Notes: Ensure aseptic operation during the sampling process to avoid cross-contamination, and record detailed sampling information, such as time, location, environmental conditions, etc.

2. Sample reception and registration

• Receiving: After the laboratory receives the sample, it first conducts a visual inspection to confirm the integrity of the sample and the clarity of the label.
• Registration: Enter sample information into the laboratory information system, including sample number, patient information (if applicable), collection time, reception time, etc., for subsequent query and management.

3. Sample classification and grouping

• Classification: Classify and group samples according to experimental requirements and sample characteristics. Different types of samples may require different processing methods and experimental procedures.
• Grouping: Group similar samples into one group to facilitate subsequent batch processing.

4. Sample pre-processing

• Cleaning and removal of impurities: For solid samples, such as tissues or cells, cleaning may be required to remove impurities and blood on the surface. For liquid samples, filtration or centrifugation may be required to remove suspended solids or sediment.
• Crushing and grinding: For samples that require analysis of intracellular components, such as tissues or cells, crushing or grinding may be required to release intracellular materials.
• Extraction and purification: Based on the analysis goals, select appropriate extraction and purification methods to extract the target analytes from the sample and remove interfering substances.
• Derivatization: Sometimes in order to improve analytical sensitivity or selectivity, it is necessary to derivatize target analytes and convert them into a form that is easier to detect or separate.

5. Sample packaging and storage

• Packaging: Pack the processed samples according to the experimental requirements, ensuring that the label of each packed sample is clear, complete, and consistent with the registration information.
• Storage: Select appropriate storage conditions and storage time based on the characteristics of the sample and experimental requirements. Some samples need to be stored at low temperatures, while others need to be protected from light. Correct storage conditions ensure sample stability and reliability.

6. Quality control and records

• Quality control: Quality control is required at every step of sample pre-processing to ensure the accuracy and reliability of the process.
• Records: Record every step and result of sample pre-processing in detail for subsequent analysis and traceability.

7. Analysis and detection

• The pre-processed samples will eventually be analyzed and tested, such as chromatographic analysis, mass spectrometry analysis, spectral analysis, etc., to obtain the required analysis data.

2. Opentrons automated pipetting workstations and liquid handling systems can achieve sample preprocessing 1. High precision and accuracy. Opentrons automated pipetting workstations and liquid handling systems have high-precision and accurate pipetting functions. This is achieved through advanced electronic pipettes or multi-channel pipettes, which can ensure the accuracy of liquid distribution and transfer during sample pre-processing, thereby avoiding errors that may be caused by human operations. This high precision and accuracy is crucial for sample preprocessing because it directly affects the reliability and accuracy of subsequent experimental results. 2. Versatility and flexibility. Opentrons' automation platform is not limited to a single pipetting operation, but can also perform a variety of complex operations such as mixing, dispensing, dilution, and shaking. These functions are essential in the sample preprocessing process and can meet the needs of different experimental scenarios. In addition, the opentrons workstation also has good compatibility and scalability, and can support multiple types of consumables and experimental equipment, allowing users to select and match according to their own experimental needs to achieve personalized sample preprocessing solutions. 3. Programming control and automation Opentrons’ automated pipetting workstations and liquid handling systems realize automated operations through programming control. Users can write corresponding programs to control the operation of the workstation according to their own experimental needs. This automated operation not only greatly improves the efficiency of sample pre-processing, but also reduces errors and uncertainties that may be caused by manual operations. At the same time, automated operations also make the experimental process more standardized and repeatable, helping to improve the stability and reliability of experimental results. 4. Wide range of application fields The opentrons automation platform is widely used in many fields such as microbiology, molecular biology, and biochemistry. Sample pre-processing processes in these fields are often complex and tedious, requiring high-precision operations and strict control. opentrons' automation platform is capable of these tasks and provides scientific researchers with efficient and accurate sample preprocessing solutions. 5. User-friendly design and operation interface Opentrons automated pipetting workstations and liquid handling systems are usually equipped with user-friendly design and operation interface. This makes it easy for users to get started and master operating skills quickly. At the same time, some advanced models are also equipped with interactive devices such as touch screens and smart displays, allowing users to intuitively monitor the experimental process and adjust experimental parameters.

The main reasons why opentrons automated pipetting workstations and liquid handling systems enable sample preparation include high precision and accuracy, versatility and flexibility, programmable control and automation, a wide range of application fields, and user-friendly design and operating interface. These factors together make opentrons' automation platform an important tool for efficient and accurate sample preparation in the laboratory.