Types and operating methods of pipetting workstations

The pipetting workstation is an automated instrument used in biological and chemical experiments, which can help experimenters complete pipetting operations of liquids quickly and accurately. Pipetting workstations can be divided into many types according to different usage requirements and application scenarios, and each type has its own specific operation methods.

1. Types of pipetting workstations 1. Characteristics of single-channel pipetting workstations: The most basic pipetting workstation has only one liquid channel and is suitable for simple liquid operations, such as sample addition, copying, and distribution. Advantages: simple structure, easy to use, relatively low price, and is one of the commonly used instruments in laboratories. 2. Features of the multi-channel pipetting workstation: It can complete liquid operations on multiple channels at the same time, and the number of liquid suction heads and discharge heads can be freely combined according to experimental needs. Advantages: Significantly improve experimental efficiency and operational accuracy, especially suitable for high-throughput experiments and large-scale data collection. 3. Features of programmable pipetting workstation: It is developed on the basis of multi-channel pipetting workstation and realizes automated operation through preset programs. Advantages: Users can write pipetting programs according to needs and automatically perform a series of liquid operations. It is widely used in experiments with high throughput, high precision and requiring a large number of repeated operations. 4. Features of pipetting robot: A more advanced automated instrument that realizes full automation of liquid operations through the combination of multiple pipetting workstations. Advantages: It can not only complete liquid operations for a single experiment, but also achieve high-throughput automated processing of multiple experiments, greatly improving experimental efficiency, and can be linked with other equipment to complete complex experimental operations. 5. Features of the portable pipetting workstation: It is portable and suitable for use in a variety of scenarios, such as field experiments, temporary laboratories, etc. Functional modes: including conventional pipetting, dispensing, over pipetting, whole plate dilution, gradient dilution and mixing/washing.

2. Operation methods of pipetting workstations Although the specific operation methods of different types of pipetting workstations may be different, the general operating procedures are roughly similar. The following is a general operation step: 1. Preparation (1) Place the pipetting workstation Place on a stable surface and connect the power cord. (2) Make sure the workstation is clean and tidy, and there are no other objects on the desktop to interfere with it. (3) Take out the required pipette and make sure it is clean. Select the appropriate pipette capacity and set it according to the experimental needs. 2. Start-up and settings (1) Press the power button to start the pipetting workstation and wait for it to enter the main interface or startup page. (2) According to the experimental requirements, set the required pipetting parameters on the pipetting workstation, such as pipetting volume, liquid aspiration speed, liquid discharge speed, etc. 3. Prepare liquid samples (1) Place the liquid sample to be pipetted on the workstation, and ensure that the container lid is opened or removed for pipetting. 4. Pipetting operation (1) Insert the pipette into the liquid sample, press the aspiration button, and wait for the pipette to absorb the specified amount of liquid sample. Take care to ensure that the pipette tip is fully immersed in the sample and to avoid creating air bubbles. (2) Move the pipette to the target container, press the discharge button, and slowly release the liquid sample. Be careful to place the pipette bottom above the liquid level in the target container to avoid air bubbles or contamination. 5. Cleaning and completion (1) During the pipetting process, you can use cleaning solution or deionized water to clean the bottom of the pipette to avoid cross-contamination between samples. (2) After completing the pipetting operation, close the pipetting workstation, return the pipette to its original position, and clean the liquid samples and related items on the workstation.

When performing the operation of the pipetting workstation, be sure to strictly refer to the accompanying instructions or participate in the study of relevant training materials to ensure that every step of the operation is accurate, thereby ensuring the reliability of the experimental results and the safety of the operation process.