protein purification system

Protein purification systems, as core equipment in the field of biochemistry and molecular biology research, are widely used in the process of accurately extracting and highly purifying single target proteins from complex biological samples. Its importance is self-evident. Next, this article will introduce the protein purification system based on its functions and characteristics:

1. Overview

The protein purification system is a comprehensive platform that integrates multiple separation technologies and can efficiently and accurately separate and purify proteins. This system is widely used in chemistry, biology, forestry, materials science and other fields, and is an indispensable and important tool in life science research.

2. Main functions

The main functions of protein purification systems include:

1. High-efficiency separation: By using a variety of chromatography techniques (such as gel filtration, ion exchange, affinity chromatography, hydrophobic chromatography, etc.), precise separation of target proteins in complex mixtures is achieved.

2. High-purity purification: It can remove impurities in the sample, such as other proteins, nucleic acids, polysaccharides, etc., thereby obtaining high-purity target proteins.

3. Automated operation: Most modern protein purification systems have automated functions and can automatically complete steps such as sampling, programming, separation, peak collection, data processing, and report printing, significantly improving experimental efficiency and accuracy.

4. Flexible application: The system supports a variety of chromatography columns and packing materials, and can be flexibly configured and optimized according to the properties and needs of different proteins.

3. Technical characteristics

1. System pump: Using a high-performance pump head, it can provide stable and precise liquid flow and achieve reproducible isocratic or gradient elution. For example, some system pump flow rates can reach 0.01–150ml/min, and the pressure range can reach 0–5 MPa (200bar, 2900psi) or even higher.

2. Detector: Equipped with a full-wavelength detector or a continuous-wavelength UV detector, which can detect protein absorption at multiple wavelengths at the same time, improving the sensitivity and accuracy of detection. The detection wavelength range usually covers 190-740nm or 190-700nm.

3. Collector: Adopt advanced collection technology, such as square collector controlled by X-Y axis, to ensure the accuracy and efficiency of collection. There are various collection methods, which can be collected by time, number of drops, peak value and time window.

4. Control software: It has a complete chromatography method template database and simple programming functions, supports expert auxiliary software and automatic data processing functions, and can automatically generate experimental reports.

4. Application examples

Protein purification systems have been widely used in the separation and purification of various biomolecules, including but not limited to:

1. Purification of natural proteins, recombinant and fusion expressed proteins.

2. Purification of immune-related molecules such as antigens and antibodies.

3. Purification of biomolecules such as polypeptides, oligonucleotides, plasmids, and viruses.

4. Preparation of protein drugs in drug research and development.

5. Protein function analysis in basic biological research.

5. Summary

As an important tool in the field of life sciences, protein purification systems play an irreplaceable role in promoting the development of biology and medicine. With the continuous advancement of technology and the continuous expansion of applications, protein purification systems will continue to provide strong support for scientific research and industrial development.