NGS laboratory equipment

NGS (Next Generation Sequencing) laboratory equipment refers to a series of professional equipment required for high-throughput sequencing technology. These equipment cover the entire process from sample processing to data analysis. The following content will introduce various experimental equipment required at different stages:

1. Sample processing and library construction equipment

1. Refrigerated centrifuge: used for processing blood, tissue and other samples, such as separating plasma, cells, etc.

2. Micro desktop centrifuge: used for centrifugation of small volume samples, such as the purification step after DNA extraction.

3. Vortex shaker: used to mix samples or reagents to ensure uniform reaction.

4. Pipette: accurately transfer liquids, such as reagents, samples, etc., to ensure the accuracy of experimental operations.

5. Biological safety cabinet: Provide a sterile operating environment to protect experimental personnel and samples from contamination.

6. Nucleic acid extractor: Automatically extract nucleic acids (such as DNA, RNA) from samples to improve extraction efficiency and purity.

7. Ultrasonic fragmentation instrument: used to fragment long-chain DNA into short fragments suitable for sequencing.

8. Magnetic stand: Used with magnetic beads to separate and purify nucleic acids.

2. Library amplification and detection equipment

1. PCR instrument: used for PCR amplification after library construction to increase the concentration of target sequences in the library.

2. QPCR instrument (real-time fluorescence quantitative PCR instrument): used for real-time monitoring during the library amplification process and to evaluate the amplification effect.

3. Qubit 2.0 Nucleic Acid Quantifier: Accurately measures nucleic acid concentration and provides important parameters for library construction and amplification.

4. Ultra-clean workbench: Provides a local clean space for key steps in the library construction and amplification process.

3. Sequencing equipment

1. High-throughput sequencer: core equipment used to sequence libraries and generate large amounts of sequence data. Common sequencing platforms include Illumina, Ion Torrent from Thermo Fisher Scientific (formerly Life Technologies), SMRT from Pacific Biosciences, etc.

2. Sequencer supporting equipment: such as constant temperature metal bath, pure water meter, etc., used to maintain the stable environment required for the operation of the sequencer.

4. Data analysis equipment

1. High-performance computer server: used to store, process and analyze the massive data generated by sequencing.

2. Data analysis software: including bioinformatics software and databases, used for data analysis such as sequence alignment, annotation, and mutation detection.

5. Auxiliary equipment

1. Refrigerator: used to store reagents, samples, etc. to maintain their stability and activity.

2. UPS (uninterruptible power supply): ensure that experimental equipment can continue to operate in the event of a power outage and protect the safety of experimental data and equipment.

3. Movable UV lamp: used for disinfection of experimental areas to reduce the risk of contamination.

6. Automated workstation

1. With the development of technology, more and more NGS laboratories have begun to introduce automated workstations, such as NadAuto-16R fully automatic NGS workstation. These workstations integrate multiple functional modules such as sample processing, library construction, amplification, and detection, which can significantly improve experimental efficiency, reduce human errors, and adapt to the needs of large-scale sequencing projects.

When we deeply discuss the equipment configuration of the NGS (Next-Generation Sequencing) laboratory, we can easily find that its equipment system is large and sophisticated, forming a comprehensive chain covering the entire chain from fine sample processing to complex data analysis. Technology platform. Not only are these devices of various types, but they each play an indispensable role, jointly promoting the depth and accuracy of life science research.