96-well plate pipette operation skills

96-well plate pipette operation skills mainly involve preparation before adding samples, selection and use of pipettes, precautions during the sample addition process, and handling after sample addition. Here are some specific tips:

1. Preparation before adding samples

1. Ensure that materials and equipment are complete: Before starting to add samples, make sure that all required materials and equipment are ready and in good condition, including 96-well plates, pipettes, tips, reagents, labels, and record books, etc.

2. Check the experimental environment: Check whether the laboratory environment is clean and quiet to avoid unnecessary interference and pollution.

3. Prepare samples and reagents: Ensure that the quality and purity of samples and reagents meet experimental requirements. For samples and reagents stored for a long time, their validity period and storage conditions should be checked. At the same time, samples and reagents should be thoroughly mixed to avoid errors when adding samples.

2. Selection and use of pipettes

1. Choose a pipette with an appropriate range: Too large or too small a range may lead to sample addition errors, so the appropriate range should be selected based on experimental needs.

2. Check the accuracy and precision of the pipette: Before using the pipette, you should check its accuracy and precision. Calibration can be performed by weighing a standard volume of water or using a calibrator.

3. Use the pipette correctly: When using the pipette, keep it vertical and gently press the button to aspirate or release liquid. Avoid using excessive force or tilting the pipette to avoid damage to the pipette or errors.

3. Precautions during the sample addition process

1. Mark the well plate: Before adding samples, the position of each well and the corresponding sample or reagent name should be marked on the 96-well plate. This helps to avoid confusion and errors.

2. Standard operation: When using a pipette, insert the tip into the liquid and gently press the button to draw the liquid. Avoid touching the tip to the bottom or side wall of the container to avoid contaminating samples or reagents. When adding liquid to the well, you should also keep the pipette vertical and gently press the button to release the liquid, ensuring that the liquid completely enters the well and avoid bubbles or splashing.

3. Control the volume and speed of sample addition: For experiments that require repeated sample addition, ensure that the volume and speed of each sample addition are consistent. This can be done using the pipette's calibration function or by manually adjusting the pipette's buttons.

4. Processing after adding sample

1. Check the sample addition results: After the sample addition is completed, check whether the liquid volume in each well is consistent and observe whether there are bubbles, contamination or other abnormalities.

2. Record data: Record the volume, time, sample or reagent name and other information added to each well in detail, which will help subsequent data analysis and interpretation of experimental results.

3. Cleaning and disinfection: During the sample addition process, pipettes and tips should be cleaned and disinfected regularly to avoid cross-contamination. Remaining samples and reagents should be properly disposed of in accordance with laboratory regulations.

5. Other matters needing attention

1. Avoid cross-contamination: During the sample addition process, avoid taking the tip out of one hole and immediately inserting it into another hole. A new tip should be used or necessary cleaning and disinfection should be performed.

2. Keep it clean: Keep the experimental table clean and dry to avoid interference from dust and pollutants.

By following the above operating tips, the accuracy and reliability of 96-well plate pipette operation can be ensured, thereby improving the success rate of experiments and the reliability of data.